Kinase tirosina (bahasa Inggris: tyrosine kinase, EC 2.7.10) adalah sejenis enzim kinase yang mempercepat proses pemindahan gugus gamma fosfat dari molekul donor berupa nukleotida trifosfat menuju molekul target dalam reaksi fosforilasi tirosina.
Reaksi yang terjadi berupa: ATP + Tirosina --(Tirosina kinase)--> ADP + Tirosina fosfat.
dan berdampingan dengan enzim tirosina fosfatase yang memindahkan gugus fosfat dari Tirosina fosfat.
Tirosina kinase banyak ditemukan pada pencerap faktor pertumbuhan, terutama pada domain sitoplasmiknya.
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