Gélose PDA

Cet article est une ébauche concernant la microbiologie et la pomme de terre.

Vous pouvez partager vos connaissances en l’améliorant (comment ?) selon les recommandations des projets correspondants.

La gélose dextrosée à la pomme de terre (en abrégé « PDA », pour Potato dextrose agar) est un milieu de culture microbiologique courant produit à base d'infusion de pomme de terre et de dextrose.

Utilisation

C'est le milieu de culture le plus largement utilisé pour cultiver des mycètes et des bactéries qui attaquent les plantes vivantes ou la matière organique végétale en décomposition[1].

Préparation

L'infusion de pomme de terre se prépare en faisant bouillir dans l'eau 200 g de pommes de terre tranchées (lavées mais non pelées) pendant 30 minutes à 1h puis en laissant décanter le bouillon obtenu ou en le filtrant à travers un coton à fromage. On dilue ensuite en ajoutant de l'eau distillée pour un volume final d'un litre. Puis on ajoute 20 g de dextrose et autant d'agar-agar en poudre avant une stérilisation par autoclave à 100 kPa pendant 15 minutes[2].

Potato dextrose broth

Un milieu de culture similaire (en abrégé « PDB », pour Potato dextrose broth) est préparé de manière identique au PDA, mais en omettant l'agar-agar[3]. Parmi les organismes couramment cultivés sur le PDB figurent des levures, telles que Candida albicans et Saccharomyces cerevisiae, et des moisissures, telle que Aspergillus niger[3].

Notes et références

  1. ↑ (en) Harold Eddleman, Ph. D, « Making Bacteria Media from Potato », Indiana Biolab, disknet.com, (consulté le )
  2. ↑ (en) BAM Media M127: Potato Dextrose Agar,Food and Drug Administration (États-Unis).
  3. ↑ a et b (en) « Potato Dextrose Broth », Merck KGaA (version du sur Internet Archive) (web.archive.org).

Voir aussi

v · m
Milieux bactériens non sélectifs
génériques
anaérobies
Milieux bactériens sélectifs
Gram négatifs génériques
Alphaproteobacteria
Betaproteobacteria
Gammaproteobacteria
Bacillota
Actinomycetes
Milieux d'identification
Milieux fongiques (en)
Autres milieux de culture

Read other articles:

Katedral Douglas

Katedral DouglasInggris: Cathedral of St Mary of the Islecode: en is deprecated Katedral Douglas54°09′02″N 4°28′57″W / 54.150655°N 4.482393°W / 54.150655; -4.482393 (St Mary's of the Isle Catholic Church, Hill Street)LokasiHill Street, DouglasNegara Pulau ManDenominasiGereja Katolik RomaArsitekturDibangun1857–59[1]AdministrasiKeuskupan AgungKeuskupan Agung Liverpool Katedral Douglas atau yang bernama resmi Katedral Bunda Maria dari Pu...

 

Bahasa Tawoyan

PemberitahuanTemplat ini mendeteksi bahwa artikel bahasa ini masih belum dinilai kualitasnya oleh ProyekWiki Bahasa dan ProyekWiki terkait dengan subjek. Perhatian: untuk penilai, halaman pembicaraan artikel ini telah diisi sehingga penilaian akan berkonflik dengan isi sebelumnya. Harap salin kode dibawah ini sebelum menilai. {{PW Bahasa|importance=|class=}} Terjadi [[false positive]]? Silakan laporkan kesalahan ini. 14.22, Jumat, 29 Maret, 2024 (UTC) • hapus singgahan Seban...

 

Keuskupan Agung Córdoba

Keuskupan Agung Córdoba di ArgentinaArchidioecesis Cordubensis in ArgentinaArquidiócesis de CórdobaKatolik Katedral Bunda dari KenaikanLokasiNegara ArgentinaProvinsi gerejawiCórdobaStatistikLuas19.722 km2 (7.615 sq mi)Populasi- Total- Katolik(per 2006)2.016.0001,814,000 (90%)Paroki114InformasiDenominasiKatolik RomaRitusRitus RomaPendirian10 Mei 1570 (453 tahun lalu)KatedralKatedral Kenaikan di Córdoba, ArgentinaPelindungBunda dari RosarioKepemimpin...

Konstanz

Konstanz Pemandangan Konstanz Lambang kebesaranLetak Konstanz di Konstanz NegaraJermanNegara bagianBaden-WürttembergWilayahFreiburgKreisKonstanzSubdivisions15Pemerintahan • Lord MayorUlrich Burchardt (CDU)Luas • Total55,65 km2 (2,149 sq mi)Ketinggian405 m (1,329 ft)Populasi (2021-12-31)[1] • Total84.736 • Kepadatan15/km2 (39/sq mi)Zona waktuWET/WMPET (UTC+1/+2)Kode pos78462–78467Kode area telepon0753...

 

Führer Ex

2002 German filmFührer ExDirected byWinfried BonengelWritten by Winfried Bonengel Ingo Hasselbach Douglas Graham Produced byLaurens StraubClementina HegewischRainer MockertStarring Aaron Hildebrand Christian Blümel CinematographyFrank BarbianEdited byMonika SchindlerMusic byLoek DikkerMichael BeckmannProductioncompaniesMBP (Germany)Next FilmStudioCanalRelease dateAugust 31, 2002 (2002-08-31)Running time107 min.CountryGermanyLanguageGermanBudget€5 000 000 Führer Ex is a Ger...

 

Sandholm

Danish estate For the public institution for asylum seekers, see Center Sandholm. SandholmsgårdGeneral informationLocationBlovstrødCountryDenmarkCoordinates55°52′12″N 12°24′13.8″E / 55.87000°N 12.403833°E / 55.87000; 12.403833Completed1749Design and constructionArchitect(s)Lauritz de Thurah Sandholm is an estate in the parish of Blovstrød, Allerød Municipality some 30 km north of Copenhagen, Denmark. History The Sandholm estate traces its history b...

Lucrezia Borgia (opera)

Opera by Gaetano Donizetti Lucrezia BorgiaOpera by Gaetano DonizettiThérèse Tietjens in the title roleLibrettistFelice RomaniLanguageItalianBased onLucrezia Borgiaby Victor HugoPremiere26 December 1833 (1833-12-26)La Scala, Milan Lucrezia Borgia is a melodramatic opera in a prologue and two acts by Gaetano Donizetti. Felice Romani wrote the Italian libretto after the play Lucrezia Borgia by Victor Hugo, in its turn after the legend of Lucrezia Borgia. Lucrezia Borgia was firs...

 

Motilitas

Pembelahan sel. Semua sel dapat dianggap motil karena dapat membelah menjadi dua sel anakan.[1] Sitoskeleton eukariotik menginduksi sel untuk bergerak melalui permukaan cair dan lebih, membelah menjadi sel-sel baru, dan sitoskeleton membimbing pengangkutan organel dalam sel. Video ini menunjukkan sitoskeleton yang diwarnai dari potongan melintang daun Arabidopsis thaliana.[2] Motilitas adalah kemampuan suatu organisme untuk bergerak secara independen, menggunakan energi metabo...

 

Condensazione

Disambiguazione – Se stai cercando il meccanismo di difesa psicologico, vedi Condensazione (psicologia). Disambiguazione – Se stai cercando il significato geologico del termine, vedi Liquefazione delle sabbie. Disambiguazione – Se stai cercando la reazione chimica di condensazione, vedi Reazione di condensazione. Il vapore acqueo presente nell'aria condensa a contatto con la superficie fredda di una bottiglia La condensazione è la transizione di fase dalla fase aeriforme alla fase liq...

Demographics of Lesotho

Demographics of LesothoPopulation pyramid of Lesotho in 2020Population2,193,970 (2022 est.)Growth rate0.76% (2022 est.)Birth rate23.15 births/1,000 population (2022 est.)Death rate11.05 deaths/1,000 population (2022 est.)Life expectancy59.57 years • male57.57 years • female61.64 yearsFertility rate2.92 children born/woman (2022 est.)Infant mortality rate48.44 deaths/1,000 live birthsNet migration rate-4.55 migrant(s)/1,000 population (2022 est.)Age structure0–14...

 

كأس السوبر الإيطالي 2003

كأس السوبر الإيطالي 2003ملعب جاينتس الذي أستضاف المباراةالحدثكأس السوبر الإيطالي يوفنتوس ميلان الدوري الإيطالي كأس إيطاليا 1 1 بعد الوقت الإضافيفاز يوفنتوس 5–3 بركلات الجزاء الترجيحيةالتاريخ3 أغسطس 2003 (2003-08-03)الملعبملعب جاينتس، شرق راذرفورد، الولايات المتحدةالحكم�...

 

Fendiline

Non-selective calcium channel blocker FendilineClinical dataATC codeC08EA01 (WHO) Identifiers IUPAC name 3,3-diphenyl-N-(1-phenylethyl)propan-1-amine CAS Number13042-18-7 YPubChem CID3336DrugBankDB08980 NChemSpider3219 NUNIIS253D559A8KEGGD07185 YChEMBLChEMBL254832 NCompTox Dashboard (EPA)DTXSID5048473 ECHA InfoCard100.032.635 Chemical and physical dataFormulaC23H25NMolar mass315.460 g·mol−13D model (JSmol)Interactive image SMILES CC(NCCC(c1ccccc1)c2c...

约翰斯顿环礁

此條目可参照英語維基百科相應條目来扩充。 (2021年5月6日)若您熟悉来源语言和主题，请协助参考外语维基百科扩充条目。请勿直接提交机械翻译，也不要翻译不可靠、低品质内容。依版权协议，译文需在编辑摘要注明来源，或于讨论页顶部标记{{Translated page}}标签。 约翰斯顿环礁Kalama Atoll 美國本土外小島嶼 Johnston Atoll 旗幟颂歌：《星條旗》The Star-Spangled Banner約翰斯頓環礁�...

 

Onondaga people

Ethnic group For other uses, see Onondaga (disambiguation). OnondagaOnoñda’gegáTodadaho Sid Hill, Traditional Chief of the Onondaga Nation at the United Nations Permanent Forum on Indigenous IssuesRegions with significant populations United States (New York)Unknown[1] Canada (Ontario)856 (2019)[2]LanguagesEnglish, Onondaga, Other Iroquoian languages.ReligionLonghouse/Gai'hwi:io, Kanoh'hon'io, Kahni'kwi'io, other Indigenous religions, ChristianityRelated...

 

Mike Ploog

Mike Ploog (Mankato, 13 luglio 1940) è un fumettista, illustratore e disegnatore statunitense. Mike Ploog si afferma nel campo dei comic nei primi anni settanta grazie al suo talento nel disegnare e creare storie horror, fantasy e la sua esperienza nel mondo dell'animazione[1]. Si tratta di generi molto popolari in quel periodo e i lettori sono avidi di storie di genere, nuove idee e personaggi[1]. Ha la fortuna di collaborare con Will Eisner e la sua definitiva consacrazione...

Liverpool City Council

Local government body in England For the local government area of New South Wales known as Liverpool City Council, see City of Liverpool (New South Wales). Liverpool City CouncilCoat of armsCorporate logoTypeTypeMetropolitan borough council LeadershipLord MayorRichard Kemp, Liberal Democrat since 15 May 2024[1] LeaderLiam Robinson, Labour since 17 May 2023[2][3] Chief ExecutiveAndrew Lewis since June 2023[4] StructureSeats85 councillorsPolitical groups ...

 

Marcellus Empiricus

Gallo-Roman writer and physician Marcellus Empiricus, also known as Marcellus Burdigalensis (“Marcellus of Bordeaux”), was a Latin medical writer from Gaul at the turn of the 4th and 5th centuries. His only extant work is the De medicamentis, a compendium of pharmacological preparations drawing on the work of multiple medical and scientific writers as well as on folk remedies and magic. It is a significant if quirky text in the history of European medical writing, an infrequent subject of...

 

Sinner's prayer

Evangelical Christian term referring to any prayer of repentance This article is about the Christian term. For other uses, see Sinner's prayer (disambiguation). This article uses bare URLs, which are uninformative and vulnerable to link rot. Please consider converting them to full citations to ensure the article remains verifiable and maintains a consistent citation style. Several templates and tools are available to assist in formatting, such as reFill (documentation) and Citation bot (docum...

الدوري البلجيكي الدرجة الأولى 1976–77

يفتقر محتوى هذه المقالة إلى الاستشهاد بمصادر. فضلاً، ساهم في تطوير هذه المقالة من خلال إضافة مصادر موثوق بها. أي معلومات غير موثقة يمكن التشكيك بها وإزالتها. (ديسمبر 2018) الدوري البلجيكي الدرجة الأولى الموسم 1976–77 البلد بلجيكا  المنظم الاتحاد الملكي البلجيكي لكرة القدم ...

 

Historia de Albania

La historia de Albania forma parte de la historia de Europa. Durante la Antigüedad clásica, Albania estuvo habitada por varias tribus ilirias (como los ardieos, albanos, amantini, enquelios o taulantios), pero también de tribus tracias y griegas, así como de varias colonias griegas establecidas en la costa iliria. En el siglo III a. C., la zona fue anexionada por Roma y pasó a formar parte de las provincias romanas de Dalmacia, Macedonia y Mesia Superior. Posteriormente el...