In enzymology, a petromyzonol sulfotransferase (EC 2.8.2.31) is an enzyme that catalyzes the chemical reaction

3'-phosphoadenylyl sulfate + 5alpha-cholan-3alpha,7alpha,12alpha,24-tetrol ${\displaystyle \rightleftharpoons }$ adenosine 3',5'-bisphosphate + 5alpha-cholan-3alpha,7alpha,12alpha-triol 24-sulfate

Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and 5alpha-cholan-3alpha,7alpha,12alpha,24-tetrol, whereas its two products are adenosine 3',5'-bisphosphate and 5alpha-cholan-3alpha,7alpha,12alpha-triol 24-sulfate.

This enzyme belongs to the family of transferases, specifically the sulfotransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:5alpha-cholan-3alpha,7alpha,12alpha,24-te trol sulfotransferase. This enzyme is also called PZ-SULT.

• Venkatachalam KV, Llanos DE, Karami KJ, Malinovskii VA (2004). "Isolation, partial purification, and characterization of a novel petromyzonol sulfotransferase from Petromyzon marinus (lamprey) larval liver". J. Lipid Res. 45 (3): 486–95. doi:10.1194/jlr.M300346-JLR200. PMID 14657197.