In enzymology, a malonate CoA-transferase (EC 2.8.3.3) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + malonate ${\displaystyle \rightleftharpoons }$ acetate + malonyl-CoA

Thus, the two substrates of this enzyme are acetyl-CoA and malonate, whereas its two products are acetate and malonyl-CoA.

This enzyme belongs to the family of transferases, specifically the CoA-transferases. The systematic name of this enzyme class is acetyl-CoA:malonate CoA-transferase. This enzyme is also called malonate coenzyme A-transferase. This enzyme participates in beta-alanine metabolism and propanoate metabolism.

• Hayaishi O (July 1955). "Enzymatic decarboxylation of malonic acid". The Journal of Biological Chemistry. 215 (1): 125–36. PMID 14392148.
• Takamura Y, Kitayama Y (1981). "Purification and some properties of malonate decarboxylase from Pseudomonas ovalis: an oligomeric enzyme with bifunctional properties". Biochem. Int. 3: 483–491.