Carbaminohemoglobin

Carbaminohemoglobin (carbaminohaemoglobin BrE) (CO2Hb, also known as carbhemoglobin and carbohemoglobin) is a compound of hemoglobin and carbon dioxide, and is one of the forms in which carbon dioxide exists in the blood.[1] Twenty-three percent of carbon dioxide is carried in blood this way (70% is converted into bicarbonate by carbonic anhydrase and then carried in plasma, 7% carried as free CO2, dissolved in plasma).[2]

Structure

The structure of carbaminohemoglobin can be described as the binding of carbon dioxide to the amino groups of the globin chains of hemoglobin. This occurs at the N-terminals of the globin chains and at the amino sidebranches of arginine and lysine residues.[3] The process of carbon dioxide binding to hemoglobin is generally known as carbamino formation. This is the source from where the protein gets its name, as it is a combination of carbamino and hemoglobin.[4]

Function

One of the primary functions of carbaminohemoglobin is to enable the transport of carbon dioxide in the bloodstream. When carbon dioxide is produced as a waste product of cellular metabolism in tissues, the compound is diffused into the bloodstream and it works to react with hemoglobin.[5]

When the binding of molecules occurs to form carbaminohemoglobin, it allows for the transport of carbon dioxide from the tissues to the lungs. One it is in the lungs, carbon dioxide is released from carbaminohemoglobin and can be let out from the body during the exhalation process. This complete process is very important for maintaining the balance of gases in the blood and to ensure that gas exchange is being transported between tissues and organs.[6]

Interaction

Carbaminohemoglobin interacts with carbon dioxide in a process known as respiratory gas exchange. The interaction involves the binding of carbon dioxide to hemoglobin. Carbon dioxide binds to the protein chains of hemoglobin. The ability of hemoglobin to bind to both oxygen and carbon dioxide molecules is what makes it an important protein to the respiratory system in respiratory gas exchange.

The interactions between carbon dioxide and hemoglobin helps in the transport of carbon dioxide from the tissues to the lungs for eliminations. When carbon dioxide is transported from the tissues, it is produced as a waste product of a set of reactions known as cellular metabolism. Most importantly, the binding of carbon dioxide to hemoglobin plays a part in the buffering of blood pH by preventing the drop of pH due to the production of carbonic acid.[6]

Although, the carbaminohemoglobin protein interacts with another protein (like hemoglobin) found in red blood cells, this interaction only takes place in the bloodstream and its products can be expelled. Carbaminohemoglobin does not interact with DNA since DNA is a molecule that is found in cell nucleus and its function is to carry genetic information.[7]

Regulation

The formation and dissociation of the protein carbaminohemoglobin are controlled by many factors to guarantee the transport of carbon dioxide to the blood stream. A list of regulatory factors are listed below:

  1. Partial Pressure of Carbon Dioxide (pCO2): The measure of carbon dioxide within arterial or venous blood.[8] The amount of carbon dioxide in the bloodstream is influenced by the partial pressure of the molecule carbon dioxide. In tissues where cellular metabolism produces carbon dioxide, the partial pressure is higher and it leads to the binding of carbon dioxide to hemoglobin. On the other hand, in the lungs, there is a lower amount of partial pressure of carbon dioxide, which promotes the separation of carbon dioxide from hemoglobin.
  2. pH: The Bohr effect outlines how the binding and release of oxygen and carbon dioxide by hemoglobin are influenced by fluctuations of pH in the blood. When tissues metabolize, they produce carbon dioxide and acidic products, which eventually lead to a decrease in pH levels in the blood. When the pH is low, this promotes the binding of carbon dioxide to hemoglobin and facilities the transport to the lungs. On the contrary, when the pH is higher in the lungs, carbon dioxide is released from hemoglobin.[9]
  3. Temperature: A factor such as temperature can affect the binding and release of gases by hemoglobin. The effect of temperature on the binding of carbon dioxide to hemoglobin is less noticeable compared to other gases, but this factor can still have an influence on the overall regulation of gas exchange.[10]
  4. Concentration of Bicarbonate (HCO3-): A high percentage of carbon dioxide in the bloodstream is transferred in the form of bicarbonate ions. Carbonic anhydrase catalyzes the conversion of carbon dioxide and water into carbonic acid. This molecule breaks down into bicarbonate and hydrogen ions. This break down process occurs in red blood cells. Ultimately, the concentration of bicarbonate ions in the bloodstream affects the formation of the protein carbaminohemoglobin in the body.[11]

Synthesis

When the tissues release carbon dioxide into the bloodstream, around 10% is dissolved into the plasma. The rest of the carbon dioxide is carried either directly or indirectly by hemoglobin. Approximately 10% of the carbon dioxide carried by hemoglobin is in the form of carbaminohemoglobin. This carbaminohemoglobin is formed by the reaction between carbon dioxide and an amino (-NH2) residue from the globin molecule, resulting in the formation of a carbamino residue (-NH.COO−). The rest of the carbon dioxide is transported in the plasma as bicarbonate anions.[12]

Mechanism

When carbon dioxide binds to hemoglobin, carbaminohemoglobin is formed, lowering hemoglobin's affinity for oxygen via the Bohr effect. The reaction is formed between a carbon dioxide molecule and an amino residue.[12] In the absence of oxygen, unbound hemoglobin molecules have a greater chance of becoming carbaminohemoglobin. The Haldane effect relates to the increased affinity of de-oxygenated hemoglobin for H+
: offloading of oxygen to the tissues thus results in increased affinity of the hemoglobin for carbon dioxide, and H+
, which the body needs to get rid of, which can then be transported to the lung for removal. Because the formation of this compound generates hydrogen ions, haemoglobin is needed to buffer it.[12]

Hemoglobin can bind to four molecules of carbon dioxide. The carbon dioxide molecules form a carbamate with the four terminal-amine groups of the four protein chains in the deoxy form of the molecule. Thus, one hemoglobin molecule can transport four carbon dioxide molecules back to the lungs, where they are released when the molecule changes back to the oxyhemoglobin form. [6]

Hydrogen ion and oxygen-carbon dioxide coupling

When carbon dioxide diffuses as a dissolved gas from the tissue capillaries, it binds to the α-amino terminus of the globulin chain, forming Carbaminohemoglobin. Carbaminohemoglobin is able to directly stabilise the T conformation as part of the carbon dioxide Bohr effect. Deoxyhemoglobin in turn subsequently increases the uptake of carbon dioxide in the form of favouring the formation of Bicarbonate as well as Carbaminohemoglobin through the Haldane effect.[13]

Disease association

Dysfunctional or altered levels of carbaminohemoglobin do not generally cause disease or disorders. Carbaminohemoglobin is a part of the carbon dioxide transport process in the body. The levels of this protein can decrease and increase based on factors that regulate the protein in the body.[14]

A way that carbaminohemoglobin can be associated with disease is when there is a change in its level caused by a pre-existing condition or imbalance in the respiratory and metabolic systems of the human body.

Some of these existing medical conditions can be the following:

  1. Respiratory acidosis: This condition is characterized by a build up of carbon dioxide in the blood, which leads to a drop in the blood's pH. This occurs when there is an impairment in the gas exchange process, such as respiratory failure.[15]
  2. Hypoventilation: This type of condition can result in higher levels of carbaminohemoglobin. This condition can be caused by many factors, such as central nervous system disorders, and even some medications.[16]

Biological Importance

The protein carbaminohemoglobin plays an important role in the transport of carbon dioxide in the blood, and its biologically important in many functions:

  1. Transport of Carbon Dioxide: This process allows for the transport of carbon dioxide from the tissues to the lungs. It is essential for maintaining the balance of gases in the bloodstream and to guarantee the removal of waste carbon dioxide from the body.[17]
  2. Buffering Blood pH: The binding of carbon dioxide to hemoglobin plays a part in the buffering of blood pH. When tissues produce carbon dioxide, the increase in acidity is reduced by the formation of bicarbonate ions. This buffering process helps prevent a decrease in pH and helps maintain a stable environment.[17]
  3. Facilitation of Gas Exchange: Hemoglobin facilitates the exchange of gases in the lungs and tissues. In the lungs, oxygen binds to hemoglobin and carbon dioxide is released. In the tissues, carbon dioxide binds to form carbaminohemoglobin and oxygen is released. This exchange process is important because tissues need oxygen and the removal of carbon dioxide is also necessary. [18]

See also

References

  1. ^ Betts JG, Desaix P, Johnson E, Johnson JE, Korol O, Kruse D, et al. (September 13, 2023). "22.5 Transport of Gases". Anatomy & Physiology. Houston: OpenStax CNX. 22.5 Transport of gases. ISBN 978-1-947172-04-3.
  2. ^ Gas Transport in the Blood Archived 2019-01-28 at the Wayback Machine FIG. 18.11 Carbon dioxide transport
  3. ^ Lumb, AB (2017). Nunn's Applied Respiratory Physiology (8th ed.). p. 153. ISBN 9780702062940.
  4. ^ Thomas C, Lumb AB (October 2012). "Physiology of haemoglobin". Continuing Education in Anaesthesia, Critical Care & Pain. 12 (5): 251–256. doi:10.1093/bjaceaccp/mks025.
  5. ^ Groeneveld AB (June 1998). "Interpreting the venous-arterial PCO2 difference". Critical Care Medicine. 26 (6): 979–980. doi:10.1097/00003246-199806000-00002. PMID 9635634.
  6. ^ a b c Perrella M, Rossi-Bernardi L (April 1979). The determination of CO2 bound to hemoglobin as carbamate. Biophysics and Physiology of Carbon Dioxide; Symposium held at the University of Regensburg (FRG). Berlin, Heidelberg: pringer Science & Business Media. pp. 75–83. doi:10.1007/978-3-642-67572-0_8.
  7. ^ Moore EE, Johnson JL, Cheng AM, Masuno T, Banerjee A (September 2005). "Insights from studies of blood substitutes in trauma". Shock. 24 (3): 197–205. doi:10.1097/01.shk.0000180075.76766.fe. PMID 16135956.
  8. ^ Messina Z, Patrick H (2022). "Partial pressure of carbon dioxide.". StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing. PMID 31869112.
  9. ^ Jensen FB (November 2004). "Red blood cell pH, the Bohr effect, and other oxygenation-linked phenomena in blood O2 and CO2 transport". Acta Physiologica Scandinavica. 182 (3): 215–227. doi:10.1111/j.1365-201X.2004.01361.x. PMID 15491402.
  10. ^ Hertog ML, Peppelenbos HW, Evelo RG, Tijskens LM (November 1998). "A dynamic and generic model of gas exchange of respiring produce: the effects of oxygen, carbon dioxide and temperature". Postharvest Biology and Technology. 14 (3): 335–349. doi:10.1016/S0925-5214(98)00058-1.
  11. ^ Arthurs GJ, Sudhakar M (December 2005). "Carbon dioxide transport". Continuing Education in Anaesthesia, Critical Care & Pain. 5 (6): 207–210. doi:10.1093/bjaceaccp/mki050.
  12. ^ a b c Waterhouse J, Campbell I (November 2005). "Respiration: gas transfer". Anaesthesia & Intensive Care Medicine. Thoracic. 6 (11): 363–366. doi:10.1383/anes.2005.6.11.363. ISSN 1472-0299.
  13. ^ Hsia CC (January 1998). "Respiratory function of hemoglobin". The New England Journal of Medicine. 338 (4): 239–247. doi:10.1056/NEJM199801223380407. PMID 9435331.
  14. ^ Song MA, Wang L (2016). "Detection of serum carbaminohemoglobin and glycated hemoglobin" (PDF). Journal of Hainan Medical University. 22 (12): 127–130. Archived from the original on 2020-11-25. Retrieved 2023-11-28.{{cite journal}}: CS1 maint: bot: original URL status unknown (link)
  15. ^ Epstein SK, Singh N (April 2001). "Respiratory acidosis". Respiratory Care. 46 (4): 366–383. PMID 11262556.
  16. ^ Johnson RA, Morais HA (2006). "Respiratory acid–base disorders.". In DiBartola SP (ed.). Fluid, Electrolyte, and Acid–base Disorders in Small Animal Practice. Elsevier Health Sciences. ISBN 978-1-4377-0655-0.
  17. ^ a b Geers C, Gros G (April 2000). "Carbon dioxide transport and carbonic anhydrase in blood and muscle". Physiological Reviews. 80 (2): 681–715. doi:10.1152/physrev.2000.80.2.681. PMID 10747205. S2CID 606543.
  18. ^ Seki S, Goto K, Kondo T, Fukushima Y, Konishi H, Kosaka F (June 1984). "Gas exchange and facilitation of high-frequency ventilation in intrathoracic surgery". The Annals of Thoracic Surgery. 37 (6): 491–496. doi:10.1016/s0003-4975(10)61139-3. PMID 6428336.


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