The enzyme 6-acetylglucose deacetylase (EC 3.1.1.33) catalyzes the reaction

6-acetyl-D-glucose + H₂O ${\displaystyle \rightleftharpoons }$ D-glucose + acetate

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is 6-acetyl-D-glucose acetylhydrolase. This enzyme is also called 6-O-acetylglucose deacetylase.

• Duff RB, Webley DM (1958). "Metabolism of 6-O-acetyl-D-glucopyranose and other monoacetyl-sugars by strains of Bacillus megaterium and other soil organisms". Biochem. J. 70 (3): 520–528. PMC 1196700. PMID 13596370.