28 Regional Amateur League teams competed in this round. No seeds were applied in the single-leg round. The draw was held on 12 September 2023.[5] The match schedules were announced on 12 September 2023.[6] 7 seeded and 7 unseeded teams qualified for the next round. Biggest upset was Kars 36 Spor (160) eliminating Dersimspor (137). Lowest-ranked team qualified for the next round was Kars 36 Spor (160). Highest-ranked team eliminated was Dersimspor (137).
48 Third League and 14 Regional Amateur League teams competed in this round. No seeds were applied in the single-leg round. The draw was held on 19 September 2023.[8] The match schedules were announced on 19 September 2023.[9] 11 seeded and 20 unseeded teams qualified for the next round. 23 Third League and 8 Regional Amateur League teams qualified for the next round. Biggest upset was Burdur MAKÜ Spor (155) eliminating Tarsus İdman Yurdu (79). Lowest-ranked team qualified for the next round was Bigaspor (159). Highest-ranked team eliminated was Batman Petrolspor (77).
19 Second League, 23 Third League and 8 Regional Amateur League teams competed in this round. Seeds were applied in the single-leg round. Seeded teams played at home. The draw was held on 29 September 2023.[10] The match schedules were announced on 2 October 2023.[11] 13 seeded and 12 unseeded teams qualified for the next round. 11 Second League, 10 Third League and 4 Regional Amateur League teams qualified to the next round. Biggest upset was Burdur MAKÜ Spor (155) eliminating Altınordu (39). Lowest-ranked team qualified for the next round was Bigaspor (159). Highest-ranked team eliminated was Altınordu (39).
10 Super League, 11 First League, 21 Second League, 22 Third League and 4 Regional Amateur League teams competed in this round. Seeds were applied in the single-leg round. Seeded teams played at home. The draw was held on 17 October 2023.[12] The match schedules were announced on 19 October 2023.[13] 27 seeded and 7 unseeded teams qualified for the next round. 9 Super League, 9 First League, 11 Second League and 5 Third League teams qualified to the next round. Biggest upset was Bornova 1877 (135) eliminating Karacabey Belediye Spor (50). Lowest-ranked team qualified for the next round was Bornova 1877 (135). Highest-ranked team eliminated was İstanbulspor (12).
15 Super League, 16 First League, 20 Second League and 5 Third League teams competed in this round. Seeds were applied in the single-leg round. Seeded teams played at home. The draw was held on 7 November 2023.[14] The match schedules were announced on 13 November 2023.[15] 26 seeded and 2 unseeded teams qualified for the next round. 15 Super League, 11 First League and 2 Second League teams qualified to the next round. Biggest upset was Kırklarelispor (61) eliminating Altay (31). Lowest-ranked team qualified for the next round was Kırklarelispor (61). Highest-ranked team eliminated was Altay (31).
19 Super League, 11 First League and 2 Second League teams have been qualified to this round. Seeding have been applied in the single-leg round and seeded teams are going to play at home. The draw was held on 8 December 2023.[16] The match schedules were announced on 26 December 2023.[17] 12 seeded and 4 unseeded teams qualified for the next round. 13 Super League, 2 First League and 1 Second League teams qualified to the next round. Biggest upset was 24Erzincanspor (47) eliminating Adana Demirspor (4). Lowest-ranked team qualified for the next round was 24Erzincanspor (47). Highest-ranked team eliminated was Adana Demirspor (4).
13 Super League, 2 First League and 1 Second League teams have been qualified to this round. Seeding have been applied in the single-leg round. The draw was held on 22 January 2024.[18] The match schedules were announced on 26 January 2024.[19]
Eight Süper Lig teams qualified for this round. Seeding was applied in the single-leg round. The draw was held on 12 February 2024.[20] The match schedules were announced on 16 February 2024.[21]