34 Third League and 18 Regional Amateur League teams competed in this round. No seeds were applied in the single-leg round. The draw was made on 5 September 2022.[3] The match schedules were announced on 6 September 2022.[4] 15 seeded and 12 unseeded teams qualified for the next round. Biggest upset was Bigaspor (140) eliminating Akhisarspor (78). Lowest-ranked team qualified for the next round was Boyabat 1868 Spor (144). Highest-ranked team eliminated was Akhisarspor (78).
22 Second League, 29 Third League and 7 Regional Amateur League teams competed in this round. Seeds were applied in the single-leg round. Seeded teams played at home. The draw was made on 16 September 2022.[6] The match schedules were announced on 20 September 2022.[7] 16 seeded and 13 unseeded teams qualified for the next round. Biggest upset was Boyabat 1868 Spor (144) eliminating Bursaspor (40). Lowest-ranked team qualified for the next round was Boyabat 1868 Spor (144). Highest-ranked team eliminated was Kocaelispor (39).
7 Super League, 13 First League, 25 Second League, 22 Third League and 1 Regional Amateur League teams competed in this round. Seeds were applied in the single-leg round. Seeded teams played at home. The draw was made on 4 October 2022.[8] The match schedules were announced on 7 October 2022.[9] 27 seeded and 7 unseeded teams qualified for the next round. Biggest upset was Efeler 09 Spor (130) eliminating Altay (21). Lowest-ranked team qualified for the next round was Efeler 09 Spor (130). Highest-ranked team eliminated was Hatayspor (12).
13 Super League, 18 First League, 18 Second League and 5 Third League teams competed in this round. Seeds were applied in the single-leg round. Seeded teams played at home. The draw was made on 21 October 2022.[10] The match schedules were announced on 26 October 2022.[11] 22 seeded and 5 unseeded teams qualified for the next round. Biggest upset was Esenler Erokspor (72) eliminating Erzurumspor FK (25). Lowest-ranked team qualified for the next round was Esenler Erokspor (72). Highest-ranked team eliminated was Yeni Malatyaspor (23).
18 Super League, 10 First League and 4 Second League teams competed in this round. Seeds were applied in the single-leg round. Seeded teams played at home. The draw was made on 11 November 2022.[13] The match schedules were announced on 9 December 2022.[14] 13 seeded and 3 unseeded teams qualified for the next round. Biggest upset was Karacabey Belediye Spor (45) eliminating Giresunspor (16). Lowest-ranked team qualified for the next round was Karacabey Belediye Spor (45). Highest-ranked team eliminated was Adana Demirspor (10).
14 Super League, 1 First League and 1 Second League teams competed in this round. Seeds were applied in the single-leg round. The draw was made on 23 December 2022.[15] The match schedules were announced on 6 January 2023.[16] 4 seeded and 4 unseeded teams qualified for the next round. Biggest upset was Gaziantep FK (15) eliminating Konyaspor (4). Lowest-ranked team qualifying for the next round was MKE Ankaragücü (17). Highest-ranked team eliminated was Konyaspor (4).
Note: Gaziantep FK withdrew from the tournament due to the 2023 Turkey–Syria earthquake.[12] Sivasspor, therefore, were awarded a 3–0 win, advancing to the semi-finals.