The 1927 All-Missouri Valley Conference football team consists of American football players chosen by various organizations for All-Missouri Valley Conference teams for the 1927 college football season. The selectors for the 1927 season included the Associated Press (AP).

• Roy LeCrone, Oklahoma (AP-1)
• Miller Brown, Missouri (AP-1)
• Harold Hauser, Kansas (AP-2)
• Ted Fleck, Kansas State (AP-2)

• Ray Randels, Nebraska (AP-1)
• Norris, Oklahoma (AP-1)
• William W. Smith, Missouri (AP-2)
• Francis E. Lucas, Missouri (AP-2)

• Danny McMullen, Nebraska (AP-1)
• Robert N. Miller, Missouri (AP-1)
• Bob Fischer, Iowa State (AP-2)
• Elmer Holm, Nebraska (AP-2)

• Gould Ayres, Iowa State (AP-1)
• Theodore James, Nebraska (AP-2)

• Wales, Iowa State (AP-1)
• A. Lynwood Haskins, Oklahoma (AP-2)

• Robert Mehrle, Missouri (AP-1)
• Glenn Presnell, Nebraska (AP-1)
• Harry Lindblom, Iowa State (AP-2)
• Joe Holsinger, Kansas State (AP-2)

• Blue Howell, Nebraska (AP-1)
• John Miller, Iowa State (AP-2)

AP = Associated Press^[1]

• 1927 College Football All-America Team