In enzymology, a 15-hydroxyicosatetraenoate dehydrogenase (EC 1.1.1.232) is an enzyme that catalyzes the chemical reaction

(15S)-15-hydroxy-5,8,11-cis-13-trans-icosatetraenoate + NAD(P)+ ${\displaystyle \rightleftharpoons }$ 15-oxo-5,8,11-cis-13-trans-icosatetraenoate + NAD(P)H + H⁺

The 3 substrates of this enzyme are 15-hydroxyicosatetraenoic acid (i.e. 15(S)-15-hydroxy-5,8,11-cis-13-trans-icosatetraenoate), NAD⁺, and NADP⁺, whereas its 4 products are 15-oxo-5,8,11-cis-13-trans-icosatetraenoate, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is (15S)-15-hydroxy-5,8,11-cis-13-trans-icosatetraenoate:NAD(P)⁺ 15-oxidoreductase. This enzyme is also called 15-hydroxyeicosatetraenoate dehydrogenase. This enzyme participates in arachidonic acid metabolism.

• Sok DE, Kang JB, Shin HD (1988). "15-Hydroxyeicosatetraenoic acid dehydrogenase activity in microsomal fraction of mouse liver homogenate". Biochem. Biophys. Res. Commun. 156 (1): 524–9. doi:10.1016/S0006-291X(88)80873-8. PMID 3052453.