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renin
renin
Identifiers
EC no.	3.4.23.15
CAS no.	9015-94-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
PMC
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PMC	articles
PubMed	articles
NCBI	proteins

REN
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	4XX4, 1BBS, 1BIL, 1BIM, 1HRN, 1RNE, 2BKS, 2BKT, 2FS4, 2G1N, 2G1O, 2G1R, 2G1S, 2G1Y, 2G20, 2G21, 2G22, 2G24, 2G26, 2G27, 2I4Q, 2IKO, 2IKU, 2IL2, 2REN, 2V0Z, 2V10, 2V11, 2V12, 2V13, 2V16, 2X0B, 3D91, 3G6Z, 3G70, 3G72, 3GW5, 3K1W, 3KM4, 3O9L, 3OAD, 3OAG, 3OOT, 3OQF, 3OQK, 3OWN, 3Q3T, 3Q4B, 3Q5H, 3SFC, 3VCM, 3VSW, 3VSX, 3VUC, 3VYD, 3VYE, 3VYF, 4AMT, 4GJ5, 4GJ6, 4GJ7, 4GJ8, 4GJ9, 4GJA, 4GJB, 4GJC, 4GJD, 4PYV, 4Q1N, 4RYC, 4RYG, 4RZ1, 4S1G, 4XX3
Identifiers
Aliases	REN, HNFJ2, renin, ADTKD4, RTD
External IDs	OMIM: 179820; MGI: 97898; HomoloGene: 20151; GeneCards: REN; OMA:REN - orthologs
Gene location (Human)
Chr.	Chromosome 1 (human)
End	204,190,324 bp
RNA expression pattern
	Top expressed in
	decidua; ; human kidney; ; stromal cell of endometrium; ; left ovary; ; testicle; ; right ovary; ; renal cortex; ; metanephros; ; metanephric glomerulus; ; body of uterus;
	n/a
	More reference expression data
	n/a
Gene ontology
Molecular function	insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; endopeptidase activity; peptidase activity; signaling receptor binding; hydrolase activity; protein binding;
Cellular component	cytoplasm; membrane; plasma membrane; intracellular anatomical structure; extracellular region; extracellular space; apical part of cell;
Biological process	response to immobilization stress; regulation of blood volume by renin-angiotensin; male gonad development; regulation of MAPK cascade; kidney development; renin-angiotensin regulation of aldosterone production; cell maturation; amyloid-beta metabolic process; response to organic substance; angiotensin maturation; proteolysis; mesonephros development; regulation of blood pressure; response to lipopolysaccharide; protein catabolic process; drinking behavior; hormone-mediated signaling pathway; response to cGMP; response to cAMP;
	Sources:Amigo / QuickGO
Orthologs
	5972
	19701
	ENSG00000143839
	ENSMUSG00000070645
	P00797
	P06281
	NM_000537
	NM_031192
	NP_000528
	NP_112469
	Wikidata
View/Edit Human	View/Edit Mouse

Renin (etymology and pronunciation), also known as an angiotensinogenase, is an aspartic protease protein and enzyme secreted by the kidneys that participates in the body's renin-angiotensin-aldosterone system (RAAS)—also known as the renin-angiotensin-aldosterone axis—that increases the volume of extracellular fluid (blood plasma, lymph, and interstitial fluid) and causes arterial vasoconstriction. Thus, it increases the body's mean arterial blood pressure.

Renin is not commonly referred to as a hormone, although it has a receptor, the (pro)renin receptor, also known as the renin receptor and prorenin receptor (see also below),^[4] as well as enzymatic activity with which it hydrolyzes angiotensinogen to angiotensin I.

Biochemistry and physiology

Structure

The primary structure of renin precursor consists of 406 amino acids with a pre- and a pro-segment carrying 20 and 46 amino acids, respectively. Mature renin contains 340 amino acids and has a mass of 37 kDa.^[5]

Secretion

The enzyme renin is secreted by pericytes in the vicinity of the afferent arterioles and similar microvessels of the kidney from specialized cells of the juxtaglomerular apparatus—the juxtaglomerular cells, in response to three stimuli:

A decrease in arterial blood pressure (that could be related to a decrease in blood volume) as detected by baroreceptors (pressure-sensitive cells). This is the most direct causal link between blood pressure and renin secretion (the other two methods operate via longer pathways).
A decrease in sodium load delivered to the distal tubule. This load is measured by the macula densa of the juxtaglomerular apparatus.
Sympathetic nervous system activity, which also controls blood pressure, acting through the β₁ adrenergic receptors.

Human renin is secreted by at least 2 cellular pathways: a constitutive pathway for the secretion of the precursor prorenin and a regulated pathway for the secretion of mature renin.^[6]

Renin–angiotensin system

The renin enzyme circulates in the bloodstream and hydrolyzes (breaks down) angiotensinogen secreted from the liver into the peptide angiotensin I.

Angiotensin I is further cleaved in the lungs by endothelial-bound angiotensin-converting enzyme (ACE) into angiotensin II, the most vasoactive peptide.^[8]^[9] Angiotensin II is a potent constrictor of all blood vessels. It acts on the smooth muscle and, therefore, raises the resistance posed by these arteries to the heart, and so for the same cardiac output, the blood pressure will rise. Angiotensin II also acts on the adrenal glands and releases aldosterone, which stimulates the epithelial cells in the distal tubule and collecting ducts of the kidneys to increase re-absorption of sodium, exchanging with potassium to maintain electrochemical neutrality, and water, leading to raised blood volume and raised blood pressure. The RAS also acts on the CNS to increase water intake by stimulating thirst, as well as conserving blood volume, by reducing urinary loss through the secretion of vasopressin from the posterior pituitary gland.

The normal concentration of renin in adult human plasma is 1.98–24.6 ng/L in the upright position.^[10]

Function

Renin activates the renin–angiotensin system by using its endopeptidase activity to cleave the peptide bonds between leucine and valine residues in angiotensinogen,^[11] produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin–converting enzyme primarily within the capillaries of the lungs. Angiotensin II then constricts blood vessels, increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex, each leading to an increase in blood pressure. Renin's primary function is therefore to eventually cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys.

Renin is secreted from juxtaglomerular kidney cells, which sense changes in renal perfusion pressure, via stretch receptors in the vascular walls. The juxtaglomerular cells are also stimulated to release renin by signaling from the macula densa. The macula densa senses changes in sodium delivery to the distal tubule, and responds to a drop in tubular sodium load by stimulating renin release in the juxtaglomerular cells. Together, the macula densa and juxtaglomerular cells comprise the juxtaglomerular complex.

Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through β₁ adrenoreceptor activation.^[12]

The (pro)renin receptor to which renin and prorenin bind is encoded by the gene ATP6ap2, ATPase H(+)-transporting lysosomal accessory protein 2, which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin as well as non-hydrolytic activation of prorenin via a conformational change in prorenin which exposes the catalytic site to angiotensinogen substrate. In addition, renin and prorenin binding results in phosphorylation of serine and tyrosine residues of ATP6AP2.^[13]

The level of renin mRNA appears to be modulated by the binding of HADHB, HuR and CP1 to a regulatory region in the 3' UTR.^[14]

Genetics

The gene for renin, REN, spans 12 kb of DNA and contains 8 introns.^[15] It produces several mRNA that encode different REN isoforms.

Mutations in the REN gene can be inherited, and are a cause of a rare inherited kidney disease, so far found to be present in only 2 families. This disease is autosomal dominant, meaning that it is characterized by a 50% chance of inheritance and is a slowly progressive chronic kidney disease that leads to the need for dialysis or kidney transplantation. Many—but not all—patients and families with this disease have an elevation in serum potassium and unexplained anemia relatively early in life. Patients with a mutation in this gene can have a variable rate of loss of kidney function, with some individuals going on dialysis in their 40s while others may not go on dialysis until into their 70s. This is a rare inherited kidney disease that exists in less than 1% of people with kidney disease.^[16]

Clinical applications

An over-active renin-angiotensin system leads to vasoconstriction and retention of sodium and water. These effects lead to hypertension. Therefore, renin inhibitors can be used for the treatment of hypertension.^[17]^[18] This is measured by the plasma renin activity (PRA).

In current medical practice, the renin–angiotensin–aldosterone system's overactivity (and resultant hypertension) is more commonly reduced using either ACE inhibitors (such as ramipril and perindopril) or angiotensin II receptor blockers (ARBs, such as losartan, irbesartan or candesartan) rather than a direct oral renin inhibitor. ACE inhibitors or ARBs are also part of the standard treatment after a heart attack.

The differential diagnosis of kidney cancer in a young patient with hypertension includes juxtaglomerular cell tumor (reninoma), Wilms' tumor, and renal cell carcinoma, all of which may produce renin.^[19]

Measurement

Renin is usually measured as the plasma renin activity (PRA). PRA is measured specially in case of certain diseases that present with hypertension or hypotension. PRA is also raised in certain tumors.^[20] A PRA measurement may be compared to a plasma aldosterone concentration (PAC) as a PAC/PRA ratio.

Discovery and naming

The name renin = ren + -in, "kidney" + "compound". The most common pronunciation in English is /ˈriːnɪn/ (long e); /ˈrɛnɪn/ (short e) is also common, but using /ˈriːnɪn/ allows one to reserve /ˈrɛnɪn/ for rennin. Renin was discovered, characterized, and named in 1898 by Robert Tigerstedt, Professor of Physiology, and his student, Per Bergman, at the Karolinska Institute in Stockholm.^[21]^[22]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000143839 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Nguyen G (Mar 2011). "Renin, (pro)renin and receptor: an update". Clin Sci (Lond). 120 (5): 169–178. doi:10.1042/CS20100432. PMID 21087212.
^ Imai T, Miyazaki H, Hirose S, Hori H, Hayashi T, Kageyama R, Ohkubo H, Nakanishi S, Murakami K (Dec 1983). "Cloning and sequence analysis of cDNA for human renin precursor". Proceedings of the National Academy of Sciences of the United States of America. 80 (24): 7405–7409. Bibcode:1983PNAS...80.7405I. doi:10.1073/pnas.80.24.7405. PMC 389959. PMID 6324167.
^ Pratt RE, Flynn JA, Hobart PM, Paul M, Dzau VJ (Mar 1988). "Different secretory pathways of renin from mouse cells transfected with the human renin gene". The Journal of Biological Chemistry. 263 (7): 3137–3141. doi:10.1016/S0021-9258(18)69046-5. PMID 2893797.^{[permanent dead link‍]}
^ Boulpaep EL, Boron WF (2005). "Integration of Salt and Water Balance; The Adrenal Gland". Medical physiology: a cellular and molecular approach. St. Louis, MO: Elsevier Saunders. pp. 866–867, 1059. ISBN 978-1-4160-2328-9.
^ Fujino T, Nakagawa N, Yuhki K, Hara A, Yamada T, Takayama K, Kuriyama S, Hosoki Y, Takahata O, Taniguchi T, Fukuzawa J, Hasebe N, Kikuchi K, Narumiya S, Ushikubi F (Sep 2004). "Decreased susceptibility to renovascular hypertension in mice lacking the prostaglandin I2 receptor IP". The Journal of Clinical Investigation. 114 (6): 805–812. doi:10.1172/JCI21382. PMC 516260. PMID 15372104.
^ Brenner & Rector's The Kidney, 7th ed., Saunders, 2004, pp. 2118-2119 Full Text with MDConsult subscription Archived 2015-02-07 at the Wayback Machine
^ "Laboratory Reference Centre Manual". Hamilton Regional Laboratory Medicine Program.^{[permanent dead link‍]}
^ Zhou MS, Schulman IH, Zeng Q (October 2012). "Link between the renin-angiotensin system and insulin resistance: implications for cardiovascular disease". Vascular Medicine. 17 (5): 330–341. doi:10.1177/1358863X12450094. PMID 22814999.
^ Kopp U, Aurell M, Nilsson IM, Ablad B (September 1980). "The role of beta-1-adrenoceptors in the renin release response to graded renal sympathetic nerve stimulation". Pflügers Archiv. 387 (2): 107–113. doi:10.1007/BF00584260. PMID 6107894. S2CID 25873845.
^ Nguyen G, Delarue F, Burcklé C, Bouzhir L, Giller T, Sraer JD (Jun 2002). "Pivotal role of the renin/prorenin receptor in angiotensin II production and cellular responses to renin". The Journal of Clinical Investigation. 109 (11): 1417–1427. doi:10.1172/JCI14276. PMC 150992. PMID 12045255.
^ Adams DJ, Beveridge DJ, van der Weyden L, Mangs H, Leedman PJ, Morris BJ (Nov 2003). "HADHB, HuR, and CP1 bind to the distal 3'-untranslated region of human renin mRNA and differentially modulate renin expression". The Journal of Biological Chemistry. 278 (45): 44894–44903. doi:10.1074/jbc.M307782200. PMID 12933794.
^ Hobart PM, Fogliano M, O'Connor BA, Schaefer IM, Chirgwin JM (Aug 1984). "Human renin gene: structure and sequence analysis". Proceedings of the National Academy of Sciences of the United States of America. 81 (16): 5026–5030. Bibcode:1984PNAS...81.5026H. doi:10.1073/pnas.81.16.5026. PMC 391630. PMID 6089171.
^ Zivná M, Hůlková H, Matignon M, Hodanová K, Vylet'al P, Kalbácová M, Baresová V, Sikora J, Blazková H, Zivný J, Ivánek R, Stránecký V, Sovová J, Claes K, Lerut E, Fryns JP, Hart PS, Hart TC, Adams JN, Pawtowski A, Clemessy M, Gasc JM, Gübler MC, Antignac C, Elleder M, Kapp K, Grimbert P, Bleyer AJ, Kmoch S (2009). "Dominant renin gene mutations associated with early-onset hyperuricemia, anemia, and chronic kidney failure". Am. J. Hum. Genet. 85 (2): 204–213. doi:10.1016/j.ajhg.2009.07.010. PMC 2725269. PMID 19664745.
^ Presentation on Direct Renin Inhibitors as Antihypertensive Drugs Archived 2010-12-07 at the Wayback Machine
^ Ram CV (Sep 2009). "Direct inhibition of renin: a physiological approach to treat hypertension and cardiovascular disease". Future Cardiology. 5 (5): 453–465. doi:10.2217/fca.09.31. PMID 19715410.
^ Méndez GP, Klock C, Nosé V (Feb 2011). "Juxtaglomerular cell tumor of the kidney: case report and differential diagnosis with emphasis on pathologic and cytopathologic features". International Journal of Surgical Pathology. 19 (1): 93–98. doi:10.1177/1066896908329413. PMID 19098017. S2CID 38702564.
^ Hamilton Regional Laboratory Medicine Program - Laboratory Reference Centre Manual. Renin Direct.
^ Phillips MI, Schmidt-Ott KM (Dec 1999). "The Discovery of Renin 100 Years Ago". News in Physiological Sciences. 14 (6): 271–274. doi:10.1152/physiologyonline.1999.14.6.271. PMID 11390864. S2CID 22118033.
^ Tigerstedt R, Bergman PG (1898). "Niere und Kreislauf" [Kidney and Circulation]. Skandinavisches Archiv für Physiologie (in German). 8: 223–271. doi:10.1111/j.1748-1716.1898.tb00272.x.

External links

The MEROPS online database for peptidases and their inhibitors: A01.007^{[permanent dead link‍]}
Renin at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
Overview of all the structural information available in the PDB for UniProt: P00797 (Renin) at the PDBe-KB.