Daffy runs a diner near Guadalajara, serving mouseburgers to cats with rubber mice as substitutes for actual mice, as he hasn't seen one in ages. However, one angry cat named El Supremo discovers the trick and demands a real mouseburger. So by gunpoint, Daffy goes to find a mouse. At this point, Daffy encounters Speedy, begging for food, who he tries to cook.
The mouse discovers his intentions and escapes to the desert, with Daffy in hot pursuit. Daffy is foiled each time by Speedy running up a cactus, Daffy accidentally knocking a cactus on himself, and being scared by Speedy into a trash can, prompting the waste management official to think he has gone crazy after Daffy tells him to put him down from within the can. (Speedy also doesn't appear for the rest of the short after that.)
Daffy returns and tries to escape, but the El Supremo stops him. Finally, El Supremo demands his burger within two minutes, forcing Daffy to serve himself as a replacement. He states, "You never know what you'll do, until you've got a gun pointed at your head" (which was almost the same line Daffy used in Golden Yeggs).
References
^Beck, Jerry; Friedwald, Will (1989). Looney Tunes and Merrie Melodies: A Complete Illustrated Guide to the Warner Bros. Cartoons. Henry Holt and Co. p. 360. ISBN0-8050-0894-2.