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Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Pektinska lijaza
Pektinska lijaza
Identifikatori
EC broj	4.2.2.10
CAS broj	9033-35-6
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Pektinska lijaza (EC 4.2.2.10, pectin trans-eliminaza, endo-pektin lijaza, polimetilgalacturonska transeliminaza, pectin metiltranseliminaza, pectolijaza, PL, PNL, PMGL) je enzim sa sistematskim imenom (1->4)-6-O-metil-alfa-D-galakturonan lijaza.^[1]^[2]^[3]^[4] Ovaj enzim katalizuje sledeću hemijsku reakciju

Eliminativno razlaganje (1->4)-alfa-D-galakturonan metil estra čime se formiraju oligosaharidi sa 4-dezoksi-6-O-metil-alfa-D-galakt-4-enuronozil grupama na njihovim neredukujućim krajevima

Ovaj enzim je reaktivniji na pektinu u odnosu na pectat.

Reference

^ Albersheim, P., Neukom, H. and Deuel, H. (1960). „Über die Bildung von ungesättigten Abbauprodukten durch ein pekinabbauendes Enzym”. Helv. Chim. Acta. 43: 1422—1426.
^ Mayans, O., Scott, M., Connerton, I., Gravesen, T., Benen, J., Visser, J., Pickersgill, R. and Jenkins, J. (1997). „Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases”. Structure. 5: 677—689. PMID 9195887.
^ Kester, H.C.M & Visser, J. (1994). „Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger”. FEMS Microbiol. Lett. 120: 63—68.
^ Mutenda, K.E., Körner, R., Christensen, T.M.I.E., Mikkelsen, J. and Roepstorff, P. (2002). „Application of mass spectrometry to determine the activity and specificity of pectin lyase A”. Carbohydr. Res. 337: 1213—1223. PMID 12110197.

Literatura

Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.

Spoljašnje veze

Pectin+lyase на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Albersheim, P., Neukom, H. and Deuel, H. (1960). „Über die Bildung von ungesättigten Abbauprodukten durch ein pekinabbauendes Enzym”. Helv. Chim. Acta. 43: 1422—1426.

[2] Mayans, O., Scott, M., Connerton, I., Gravesen, T., Benen, J., Visser, J., Pickersgill, R. and Jenkins, J. (1997). „Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases”. Structure. 5: 677—689. PMID 9195887.

[3] Kester, H.C.M & Visser, J. (1994). „Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger”. FEMS Microbiol. Lett. 120: 63—68.

[4] Mutenda, K.E., Körner, R., Christensen, T.M.I.E., Mikkelsen, J. and Roepstorff, P. (2002). „Application of mass spectrometry to determine the activity and specificity of pectin lyase A”. Carbohydr. Res. 337: 1213—1223. PMID 12110197.

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