Di-trans,poli-cis-dekaprenilcistransferaza

Di-trans,poli-cis-dekaprenilcistransferaza
Identifikatori
EC broj2.5.1.31
CAS broj52350-87-5
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Di-trans,poli-cis-dekaprenilcistransferaza (EC 2.5.1.31, di-trans,poli-cis-undekaprenil-difosfat sintaza, undekaprenil-difosfat sintaza, baktoprenil-difosfatna sintaza, UPP sintetaza, undekaprenil difosfatna sintetaza, undekaprenilna pirofosfat, sintetaza) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat:izopentenil-difosfat cistransferaza (dodaje 8 izopentenilnih jedinica).[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju

(2E,6E)-farnezil difosfat + 8 izopentenil difosfat 8 difosfat + ditrans,oktacis-undekaprenil difosfat

Undekaprenil pirofosfatna sintaza katalizuje konsekutivne reakcije kondenzacije farnezil difosfata sa osam izopentenilnih difosfata, u kojima se nove cis-dvostruke veze formiraju. Time se generiše undekaprenil difosfat koji služi kao lipidni nosilac za peptidoglikansku sintezu bakterijskog ćelijskog zida.

Reference

  1. ^ Muth, J.D. & Allen, C.M. (1984). „Undecaprenyl pyrophosphate synthetase from Lactobacillus plantarum: a dimeric protein”. Arch. Biochem. Biophys. 230: 49—60. PMID 6712246. 
  2. ^ Takahashi, I. & Ogura, K. (1982). „Prenyltransferases of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase”. J. Biochem. (Tokyo). 92: 1527—1537. PMID 6818223. 
  3. ^ Guo, R.T., Ko, T.P., Chen, A.P., Kuo, C.J., Wang, A.H. and Liang, P.H. (2005). „Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis”. J. Biol. Chem. 280: 20762—20774. PMID 15788389. 
  4. ^ Ko, T.P., Chen, Y.K., Robinson, H., Tsai, P.C., Gao, Y.G., Chen, A.P., Wang, A.H. and Liang, P.H. (2001). „Mechanism of product chain length determination and the role of a flexible loop in Escherichia coli undecaprenyl-pyrophosphate synthase catalysis”. J. Biol. Chem. 276: 47474—47482. PMID 11581264. 
  5. ^ Fujikura, K., Zhang, Y.W., Fujihashi, M., Miki, K. and Koyama, T. (2003). „Mutational analysis of allylic substrate binding site of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase”. Biochemistry. 42: 4035—4041. PMID 12680756. 
  6. ^ Fujihashi, M., Zhang, Y.W., Higuchi, Y., Li, X.Y., Koyama, T. and Miki, K. (2001). „Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase”. Proc. Natl. Acad. Sci. USA. 98: 4337—4342. PMID 11287651. 
  7. ^ Pan, J.J., Chiou, S.T. and Liang, P.H. (2000). „Product distribution and pre-steady-state kinetic analysis of Escherichia coli undecaprenyl pyrophosphate synthase reaction”. Biochemistry. 39: 10936—10942. PMID 10978182. 
  8. ^ Kharel, Y., Zhang, Y.W., Fujihashi, M., Miki, K. and Koyama, T. (2003). „Significance of highly conserved aromatic residues in Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase”. J. Biochem. 134: 819—826. PMID 14769870. 

Literatura

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