MCM6

Minichromosome maintenance complex component 6
Dostupne strukture
	2KLQ, 2LE8
Identifikatori
Simboli	MCM6; MCG40308; Mis5; P105MCM
Vanjski ID	OMIM: 601806 MGI: 1298227 HomoloGene: 4322 GeneCards: MCM6 Gene
EC broj	3.6.4.12
Ontologija gena
Molekulska funkcija	• vezivanje jednolančane DNK • aktivnost ATP-zavisne DNK helikaze • proteinsko vezivanje
Ćelijska komponenta	• nukleus • nukleoplazma • nukleolus
Biološki proces	• G1/S tranzicija mitotičkog ćelijskog ciklusa • mitotički ćelijski ciklus • DNK replikacija
Pregled RNK izražavanja
	podaci
Ortolozi

edit

Faktor licenciranja DNK replikacije MCM6 je protein koji je kod ljudi kodiran MCM6 genom.^[1] MCM6 je jedan od visoko konzerviranih proteina održavanja mini-hromozoma (MCM) koji su esencijalni za inicijaciju replikacije eukariotskog genoma.

Funkcija

MCM kompleks se sastoji od MCM6 (ovog proteina) i MCM2, dok 4 i 7 poseduju DNK helikaznu aktivnost, i smatra se da deluju kao enzimi DNK odmotavanja. Heksamerni proteinski kompleks koji formiraju MCM proteini je ključna komponenta prereplikacionog kompleksa (pre-RC), i učestvuje u formiranju replikacione viljuške i u regrutovanju drugih proteina koji učestvuju u DNK replikaciji. Fosforilacija kompleksa posredstvom CDC2 kinaze redukuje helikaznu aktivnost.^[2] Nedavno je pokazano da Mcm 6 formira jake interakcije sa Cdt1. Mutiranjem ključnih amionokiselina dovodi do umanjene Cdt1 regrutacije Mcm2-7 proteinima na pre-RC.^[3]

Interakcije

MCM6 formira interakcije sa ORC1L,^[4] ORC2L,^[4] replikacionim proteinom A1,^[4] ORC4L,^[4] MCM4,^[5]^[6]^[7] MCM7,^[7]^[8] MCM2^[4]^[5]^[7]^[9]^[10] i CDC45-srodnim proteinom.^[4]

Povezano

Održavanje minihromozoma

Reference

↑ Harvey CB, Wang Y, Darmoul D, Phillips A, Mantei N, Swallow DM (December 1996). „Characterisation of a human homologue of a yeast cell division cycle gene, MCM6, located adjacent to the 5' end of the lactase gene on chromosome 2q21”. FEBS Lett. 398 (2–3): 135–40. DOI:10.1016/S0014-5793(96)01189-1. PMID 8977093.
↑ „Entrez Gene: MCM6 minichromosome maintenance deficient 6 homolog (S. cerevisiae)”.
↑ Wei Z, Liu C, Wu X, Xu N, Zhou B, Liang C, Zhu G (March 2010). „Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6”. J Biol Chem 285 (17): 12469–73. DOI:10.1074/jbc.C109.094599. PMC 2857124. PMID 20202939.
↑ ^4,0 ^4,1 ^4,2 ^4,3 ^4,4 ^4,5 Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. PMID 12614612.
↑ ^5,0 ^5,1 Yabuta N, Kajimura N, Mayanagi K, Sato M, Gotow T, Uchiyama Y, Ishimi Y, Nojima H (May 2003). „Mammalian Mcm2/4/6/7 complex forms a toroidal structure”. Genes Cells 8 (5): 413–21. DOI:10.1046/j.1365-2443.2003.00645.x. PMID 12694531.
↑ Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H (September 1996). „Binding of human minichromosome maintenance proteins with histone H3”. J. Biol. Chem. 271 (39): 24115–22. DOI:10.1074/jbc.271.39.24115. PMID 8798650.
↑ ^7,0 ^7,1 ^7,2 You Z, Ishimi Y, Masai H, Hanaoka F (November 2002). „Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex”. J. Biol. Chem. 277 (45): 42471–9. DOI:10.1074/jbc.M205769200. PMID 12207017.
↑ Fujita M, Kiyono T, Hayashi Y, Ishibashi M (April 1997). „In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP”. J. Biol. Chem. 272 (16): 10928–35. DOI:10.1074/jbc.272.16.10928. PMID 9099751.
↑ Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). „Towards a proteome-scale map of the human protein-protein interaction network”. Nature 437 (7062): 1173–8. DOI:10.1038/nature04209. PMID 16189514.
↑ You Z, Komamura Y, Ishimi Y (December 1999). „Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity”. Mol. Cell. Biol. 19 (12): 8003–15. PMC 84885. PMID 10567526.

Literatura

Ishimi Y, Ichinose S, Omori A, et al. (1996). „Binding of human minichromosome maintenance proteins with histone H3”. J. Biol. Chem. 271 (39): 24115–22. DOI:10.1074/jbc.271.39.24115. PMID 8798650.
Holthoff HP, Hameister H, Knippers R (1997). „A novel human Mcm protein: homology to the yeast replication protein Mis5 and chromosomal location”. Genomics 37 (1): 131–4. DOI:10.1006/geno.1996.0530. PMID 8921380.
Tsuruga H, Yabuta N, Hosoya S, et al. (1997). „HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5”. Genes Cells 2 (6): 381–99. DOI:10.1046/j.1365-2443.1997.1290327.x. PMID 9286856.
Ishimi Y (1997). „A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex”. J. Biol. Chem. 272 (39): 24508–13. DOI:10.1074/jbc.272.39.24508. PMID 9305914.
Holthoff HP, Baack M, Richter A, et al. (1998). „Human protein MCM6 on HeLa cell chromatin”. J. Biol. Chem. 273 (13): 7320–5. DOI:10.1074/jbc.273.13.7320. PMID 9516426.
Ohtani K, Iwanaga R, Nakamura M, et al. (1999). „Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F”. Oncogene 18 (14): 2299–309. DOI:10.1038/sj.onc.1202544. PMID 10327050.
You Z, Komamura Y, Ishimi Y (2000). „Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity”. Mol. Cell. Biol. 19 (12): 8003–15. PMC 84885. PMID 10567526.
Ishimi Y, Komamura-Kohno Y, You Z, et al. (2000). „Inhibition of Mcm4,6,7 helicase activity by phosphorylation with cyclin A/Cdk2”. J. Biol. Chem. 275 (21): 16235–41. DOI:10.1074/jbc.M909040199. PMID 10748114.
Izumi M, Yanagi K, Mizuno T, et al. (2001). „The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase”. Nucleic Acids Res. 28 (23): 4769–77. DOI:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689.
Lee JK, Hurwitz J (2001). „Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures”. Proc. Natl. Acad. Sci. U.S.A. 98 (1): 54–9. DOI:10.1073/pnas.98.1.54. PMC 14543. PMID 11136247.
Enattah NS, Sahi T, Savilahti E, et al. (2002). „Identification of a variant associated with adult-type hypolactasia”. Nat. Genet. 30 (2): 233–7. DOI:10.1038/ng826. PMID 11788828.
Yanagi K, Mizuno T, You Z, Hanaoka F (2002). „Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity”. J. Biol. Chem. 277 (43): 40871–80. DOI:10.1074/jbc.M206202200. PMID 12192004.
You Z, Ishimi Y, Masai H, Hanaoka F (2003). „Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex”. J. Biol. Chem. 277 (45): 42471–9. DOI:10.1074/jbc.M205769200. PMID 12207017.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Kneissl M, Pütter V, Szalay AA, Grummt F (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. PMID 12614612.
Yabuta N, Kajimura N, Mayanagi K, et al. (2004). „Mammalian Mcm2/4/6/7 complex forms a toroidal structure”. Genes Cells 8 (5): 413–21. DOI:10.1046/j.1365-2443.2003.00645.x. PMID 12694531.
Johnson EM, Kinoshita Y, Daniel DC (2003). „A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13”. Nucleic Acids Res. 31 (11): 2915–25. DOI:10.1093/nar/gkg395. PMC 156728. PMID 12771218.
Ramachandran N, Hainsworth E, Bhullar B, et al. (2004). „Self-assembling protein microarrays”. Science 305 (5680): 86–90. DOI:10.1126/science.1097639. PMID 15232106.
Yu Z, Feng D, Liang C (2004). „Pairwise interactions of the six human MCM protein subunits”. J. Mol. Biol. 340 (5): 1197–206. DOI:10.1016/j.jmb.2004.05.024. PMID 15236977.

[pmid8977093-1] Harvey CB, Wang Y, Darmoul D, Phillips A, Mantei N, Swallow DM (December 1996). „Characterisation of a human homologue of a yeast cell division cycle gene, MCM6, located adjacent to the 5' end of the lactase gene on chromosome 2q21”. FEBS Lett. 398 (2–3): 135–40. DOI:10.1016/S0014-5793(96)01189-1. PMID 8977093.

[entrez-2] „Entrez Gene: MCM6 minichromosome maintenance deficient 6 homolog (S. cerevisiae)”.

[pmid20202939-3] Wei Z, Liu C, Wu X, Xu N, Zhou B, Liang C, Zhu G (March 2010). „Characterization and structure determination of the Cdt1 binding domain of human minichromosome maintenance (Mcm) 6”. J Biol Chem 285 (17): 12469–73. DOI:10.1074/jbc.C109.094599. PMC 2857124. PMID 20202939.

[pmid12614612-4] 4,0 ^4,1 ^4,2 ^4,3 ^4,4 ^4,5 Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111–28. DOI:10.1016/S0022-2836(03)00079-2. PMID 12614612.

[pmid12694531-5] 5,0 ^5,1 Yabuta N, Kajimura N, Mayanagi K, Sato M, Gotow T, Uchiyama Y, Ishimi Y, Nojima H (May 2003). „Mammalian Mcm2/4/6/7 complex forms a toroidal structure”. Genes Cells 8 (5): 413–21. DOI:10.1046/j.1365-2443.2003.00645.x. PMID 12694531.

[pmid8798650-6] Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H (September 1996). „Binding of human minichromosome maintenance proteins with histone H3”. J. Biol. Chem. 271 (39): 24115–22. DOI:10.1074/jbc.271.39.24115. PMID 8798650.

[pmid12207017-7] 7,0 ^7,1 ^7,2 You Z, Ishimi Y, Masai H, Hanaoka F (November 2002). „Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex”. J. Biol. Chem. 277 (45): 42471–9. DOI:10.1074/jbc.M205769200. PMID 12207017.

[pmid9099751-8] Fujita M, Kiyono T, Hayashi Y, Ishibashi M (April 1997). „In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP”. J. Biol. Chem. 272 (16): 10928–35. DOI:10.1074/jbc.272.16.10928. PMID 9099751.

[pmid16189514-9] Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). „Towards a proteome-scale map of the human protein-protein interaction network”. Nature 437 (7062): 1173–8. DOI:10.1038/nature04209. PMID 16189514.

[pmid10567526-10] You Z, Komamura Y, Ishimi Y (December 1999). „Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity”. Mol. Cell. Biol. 19 (12): 8003–15. PMC 84885. PMID 10567526.

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