RNA-OUT

RNA-OUT
RNA-OUT
	Predicted secondary structure and sequence conservation of RNA-OUT
Identifiers
Symbol	RNA-OUT
Rfam	RF00240
Other data
RNA type	Gene; antisense
Domain(s)	Bacteria
SO	SO:0000644
PDB structures	PDBe

RNA-OUT is a non-coding RNA that is antisense to the RNA-IN non-coding RNA. Transposition of insertion sequence IS10 is regulated by an anti-sense RNA which inhibits transposase expression when IS10 is present in multiple copies per cell. IS10 antisense pairing is facilitated by the RNA-binding protein, Hfq. RNA-OUT consists of a stem-loop domain topped by a flexibly paired loop; the 5′ end of the target molecule, RNA-IN, is complementary to the top of the loop, and complementarity extends for 35 nucleotides down one side of RNA-OUT.^[1]

References

^ Kittle JD, Simons RW, Lee J, Kleckner N (December 1989). "Insertion sequence IS10 anti-sense pairing initiates by an interaction between the 5′ end of the target RNA and a loop in the anti-sense RNA". Journal of Molecular Biology. 210 (3): 561–572. doi:10.1016/0022-2836(89)90132-0. PMID 2482367.