Locke was signed to the Denver Broncos' practice squad on December 17, 2019.[5] He was waived during final roster cuts on September 5, 2020,[6] and signed to the practice squad the following day.[7] Locke was elevated to the active roster on September 14 for the team's Week 1 game against the Tennessee Titans,[8] making his NFL debut;[9] he reverted to the practice squad the next day. The Broncos promoted Locke to their active roster on September 24.[10]
Since 2020, Locke established himself as a core special teamer and led the Broncos in special teams snaps.
On March 16, 2023, Locke re-signed with Denver.[11] He was placed on short-term injured reserve on August 31,[12] and activated on October 7. On October 22, Locke made his first NFL career interception on a pass from quarterback Jordan Love to seal a Broncos win at home against the Green Bay Packers.
On March 11, 2024, Locke signed a two-year, $7 million contract extension with the Broncos.[13][14]