In enzymology, a nuatigenin 3beta-glucosyltransferase (EC 2.4.1.192) is an enzyme that catalyzes the chemical reaction

UDP-glucose + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol ${\displaystyle \rightleftharpoons }$ UDP + (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucoside

Thus, the two substrates of this enzyme are UDP-glucose and (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol, whereas its 3 products are UDP, (20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol, and 3-O-beta-D-glucoside.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucose:(20S,22S,25S)-22,25-epoxyfurost-5-ene-3beta,26-diol 3-O-beta-D-glucosyltransferase. This enzyme is also called uridine diphosphoglucose-nuatigenin glucosyltransferase.

• Kalinowska M, Wojciechowski ZA (1986). "Enzymatic-synthesis of nuatigenin 3-beta-D-glucoside in oat (Avena sativa) leaves". Phytochemistry. 25 (11): 2525–2529. doi:10.1016/S0031-9422(00)84501-X.
• Kalinowska M, Wojciechowski ZA (1987). "Subcellular-localization of UDPG-nuatigenin glucosyltransferase in oat leaves". Phytochemistry. 26 (2): 353–357. doi:10.1016/S0031-9422(00)81413-2.