In enzymology, a N-acyl-D-glutamate deacylase (EC 3.5.1.82) is an enzyme that catalyzes the chemical reaction

N-acyl-D-glutamate + H₂O ${\displaystyle \rightleftharpoons }$ a carboxylate + D-glutamate

Thus, the two substrates of this enzyme are N-acyl-D-glutamate and H₂O, whereas its two products are carboxylate and D-glutamate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-D-glutamate amidohydrolase. It employs one cofactor, zinc.

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