List of restriction enzyme cutting sites: O–R
This article contains a list of the most studied restriction enzymes whose names start with O to R inclusive. It contains approximately 130 enzymes.
The following information is given:
Enzyme : Accepted name of the molecule , according to the internationally adopted nomenclature [ 1] [ 2] , and bibliographical references . (Further reading: see the section "Nomenclature " in the article "Restriction enzyme ".)
PDB code : Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[ 3] [ 4] .
Source : Organism that naturally produces the enzyme.
Recognition sequence : Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut : Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[ 5] [ 6] .
Isoschizomers and neoschizomers : An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI ). When "None on date " is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:
EcoR70I
Whole list navigation
Restriction enzymes
O
Enzyme
PDB code
Source
Recognition sequence
Cut
Isoschizomers
OfoI
Oscillatoria foreaui A-1340
5' CYCGRG
3' GRGCYC
5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5'
AquI, AvaI, Bse15I, BspLU4I, Eco27kI, Nli3877I , PlaAI, PunAI
OkrAI
Oceanospirillum kriegii
5' GGATCC
3' CCTAGG
5' ---G GATCC--- 3'
3' ---CCTAG G--- 5'
BamHI , Bce751I, BnaI, Bsp98I, BstI, OkrAI, SolI, Uba4009I
OliI
Oceanospirillum linum 4-5D
5' CACN4 GTG
3' GTGN4 CAC
5' ---CACNN NNGTG--- 3'
3' ---GTGNN NNCAC--- 5'
AleI
OxaNI
Oerskovia xanthineolytica N
5' CCTNAGG
3' GGANTCC
5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5'
AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI
P
Enzyme
PDB code
Source
Recognition sequence
Cut
Isoschizomers
PabI
2DVY
Pyrococcus abyssi
5' GTAC
3' CATG
5' ---GTA C--- 3'
3' ---C ATG--- 5'
AfaI , Csp6I, CviQI, HpyBI ,
HpyBI, PlaAII , RsaI ,
RsaNI ,
PacI
Pseudomonas alcaligenes
5' TTAATTAA
3' AATTAATT
5' ---TTAAT TAA--- 3'
3' ---AAT TAATT--- 5'
Pac25I
Pseudomonas alcaligenes
5' CCCGGG
3' GGGCCC
5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5'
AhyI, Cfr9I, EaeAI, EclRI, PspAI, PspALI , XcyI, XmaI, XmaCI
PaeI
Pseudomonas aeruginosa
5' GCATGC
3' CGTACG
5' ---GCATG C--- 3'
3' ---C GTACG--- 5'
PaeAI
Pseudomonas aeruginosa
5' CCGCGG
3' GGCGCC
5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'
PaeBI
Pseudomonas aeruginosa - 18
5' CCCGGG
3' GGGCCC
5' ---CCC GGG--- 3'
3' ---GGG CCC--- 5'
AhyI, Cfr9I, EaeAI, EclRI, PspALI , SmaI , TspMI, XcyI, XmaI, XmaCI
PaeHI
Pseudomonas aeruginosa 4148
5' GRGCYC
3' CYCGRG
5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'
PaeHII
Pseudomonas aeruginosa 4148
5' CTGCAG
3' GACGTC
5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5'
AliAJI, BspBI, BspMAI, CfuII, Ecl2zI, HalII, Srl5DI, Sst12I, XcpI
PaeQI
Pseudomonas aeruginosa Q2
5' CCGCGG
3' GGCGCC
5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'
PaeR7I
Pseudomonas aeruginosa PA0303 pMG7
5' CTCGAG
3' GAGCTC
5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5'
AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI
Pae2kI
Pseudomonas aeruginosa 2k
5' AGATCT
3' TCTAGA
5' ---A GATCT--- 3'
3' ---TCTAG A--- 5'
Pae5kI
Pseudomonas aeruginosa 5k
5' CCGCGG
3' GGCGCC
5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'
Pae14kI
Pseudomonas aeruginosa 14k
5' CCGCGG
3' GGCGCC
5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'
Pae17kI
Pseudomonas aeruginosa 17k
5' CAGCTG
3' GTCGAC
5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'
Pae18kI
Pseudomonas aeruginosa 18k
5' AGATCT
3' TCTAGA
5' ---A GATCT--- 3'
3' ---TCTAG A--- 5'
PagI
Pseudomonas alcaligenes Sau 14-027
5' TCATGA
3' AGTACT
5' ---T CATGA--- 3'
3' ---AGTAC T--- 5'
PalI
Providencia alcalifaciens
5' GGCC
3' CCGG
5' ---GG CC--- 3'
3' ---CC GG--- 5'
PamI
Phormidium ambiguum
5' TGCGCA
3' ACGCGT
5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5'
Acc16I, AosI, AviII, FdiII, FspI, MstI, NsbI, Pun14627I
PamII
Phormidium ambiguum
5' GRCGYC
3' CYGCRG
5' ---GR CGYC--- 3'
3' ---CYGC RG--- 5'
AsuIII, BsaHI, BstACI, HgiDI, HgiGI, HgiHII, Hin1I, Hsp92I, Msp17I
PanI
Pseudomonas alkanolytica
5' CTCGAG
3' GAGCTC
5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5'
BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
PasI
Pseudomonas anguilliseptica RFL1
5' CCCWGGG
3' GGGWCCC
5' ---CC CWGGG--- 3'
3' ---GGGWC CC--- 5'
— None on May 2010 —
PauI
Paracoccus alcaliphilus ZVK3-3
5' GCGCGC
3' CGCGCG
5' ---G CGCGC--- 3'
3' ---CGCGC G--- 5'
PauAI
Phormidium autumnale
5' RCATGY
3' YGTACR
5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'
PauAII
Phormidium autumnale
5' TTTAAA
3' AAATTT
5' ---TTT AAA--- 3'
3' ---AAA TTT--- 5'
AhaIII, DraI, SruI
PceI
Planococcus citreus 55
5' AGGCCT
3' TCCGGA
5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5'
AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI
PciI
Planococcus citreus SE-F45
5' ACATGT
3' TGTACA
5' ---A CATGT--- 3'
3' ---TGTAC A--- 5'
PctI
Planococcus citreus SM
5' GAATGC
3' CTTACG
5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5'
Asp26HI, Asp27HI, Asp35HI
BmaHI,
BsaMI, BscCI, Mva1269I
Pde12I
Paracoccus denitrificans 12
5' GGNCC
3' CCNGG
5' ---G GNCC--- 3'
3' ---CCNG G--- 5'
AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbI
Pde133I
Paracoccus denitrificans 133
5' GGCC
3' CCGG
5' ---GG CC--- 3'
3' ---CC GG--- 5'
Pde137I
Paracoccus denitrificans 137
5' CCGG
3' GGCC
5' ---C CGG--- 3'
3' ---GGC C--- 5'
PdiI
Pseudomonas diminuta Mck 33-321
5' GCCGGC
3' CGGCCG
5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'
PdmI
Pseudomonas diminuta Auk 5-324
5' GAAN4 TTC
3' CTTN4 AAG
5' ---GAANN NNTTC--- 3'
3' ---CTTNN NNAAG--- 5'
Asp700I, BbvAI, MroXI, XmnI
PfaAI
Phormidium favosum
5' GGYRCC
3' CCRYGG
5' ---G GYRCC--- 3'
3' ---CCRYG G--- 5'
AccB1I, BanI, BbvBI, BshNI, Eco64I, HgiCI, HgiHI, MspB4I
PfaAII
Phormidium favosum
5' CATATG
3' GTATAC
5' ---CA TATG--- 3'
3' ---GTAT AC--- 5'
PfaAIII
Phormidium favosum
5' GCATGC
3' CGTACG
5' ---GCATG C--- 3'
3' ---C GTACG--- 5'
PfeI
Psychrobacter faecalis RFL1
5' GAWTC
3' CTWAG
5' ---G AWTC--- 3'
3' ---CTWA G--- 5'
Pfl8I
Pseudomonas fluorescens 8
5' GGATCC
3' CCTAGG
5' ---G GATCC--- 3'
3' ---CCTAG G--- 5'
AliAJI, BspBI, BsuBI, CflI, CfrA4I, PaePI, Pfl21I, PstI, SflII, Sst12I
Pfl21I
Pseudomonas sp. 21
5' CTGCAG
3' GACGTC
5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5'
Pfl23II
Pseudomonas fluorescens RFL23
5' CGTACG
3' GCATGC
5' ---C GTACG--- 3'
3' ---GCATG C--- 5'
Pfl27I
Pseudomonas fluorescens RFL27
5' RGGWCCY
3' YCCWGGR
5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'
PflBI
Pseudomonas fluorescens type B
5' CCAN5 TGG
3' GGTN5 ACC
5' ---CCANNNN NTGG--- 3'
3' ---GGTN NNNNACC--- 5'
AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflMI, Van91I
PflFI
Pseudomonas fluorescens biotype F
5' GACNNNGTC
3' CTGNNNCAG
5' ---GACN NNGTC--- 3'
3' ---CTGNN NCAG--- 5'
AspI, AtsI, PsyI, TelI, Tth111I
PflKI
Pseudomonas fluorescens
5' GGCC
3' CCGG
5' ---GG CC--- 3'
3' ---CC GG--- 5'
PflMI
Pseudomonas fluorescens
5' CCAN5 TGG
3' GGTN5 ACC
5' ---CCANNNN NTGG--- 3'
3' ---GGTN NNNNACC--- 5'
AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, Van91I
PfoI
Pseudomonas fluorescens biovar 126
5' TCCNGGA
3' AGGNCCT
5' ---T CCNGGA--- 3'
3' ---AGGNCC T--- 5'
PgaI
Pseudomonas gladioli B4
5' ATCGAT
3' TAGCTA
5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5'
BanIII, BbvAII, BliAI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I
PhaI
Pasteurella haemolytica
5' GCATC
3' CGTAG
5' ---GCATCN4 N NNNN--- 3'
3' ---CGTAGN4 NNNNN --- 5'
PhoI
Pyrococcus horikoshii OT3
5' GGCC
3' CCGG
5' ---GG CC--- 3'
3' ---CC GG--- 5'
PinAI
Pseudomonas inequalis
5' ACCGGT
3' TGGCCA
5' ---A CCGGT--- 3'
3' ---TGGCC A--- 5'
AgeI, AsiAI,
AsiGI,
BshTI
CsiAI, CspAI
PinBI
Phormidium inundatum
5' ATGCAT
3' TACGTA
5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5'
EcoT22I, BfrBI, Mph1103I, NsiI, Ppu10I , SepI, SspD5II, Zsp2I
PinBII
Phormidium inundatum
5' TCCGGA
3' AGGCCT
5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5'
Aor13HI, BlfI, Bsp13I, BspEI, Bsu23I, Kpn2I, PinBII, PtaI
PlaI
Phormidium lapideum
5' GGCC
3' CCGG
5' ---GG CC--- 3'
3' ---CC GG--- 5'
PlaII
Phormidium lapideum
5' TTCGAA
3' AAGCTT
5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5'
Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, SfuI, SspRFI
PlaAI
Phormidium laminosum
5' CYCGRG
3' GRGCYC
5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5'
AquI, BcoI, Bse15I, BspLU4I, Eco27kI, Nli3877I , OfoI, PunAI
PlaAII
Phormidium laminosum
5' GTAC
3' CATG
5' ---GT AC--- 3'
3' ---CA TG--- 5'
AfaI, Csp6I , CviQI , CviRII ,
HpyBI, PabI , RsaI,
RsaNI ,
PleI
Pseudomonas lemoignei
5' GAGTC
3' CTCAG
5' ---GAGTCNNNN N--- 3'
3' ---CTCAGNNNNN --- 5'
Ple19I
Pseudomonas lemoignei 19
5' CGATCG
3' GCTAGC
5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5'
Afa22MI, BspCI, ErhB9I, MvrI, Psu161I, PvuI, RshI, XorII
PmaCI
Pseudomonas maltophilia CB50P
5' CACGTG
3' GTGCAC
5' ---CAC GTG--- 3'
3' ---GTG CAC--- 5'
AcvI, BcoAI, BbrPI, Eco72I, PmlI, PspCI
PmeI
Pseudomonas mendocina
5' GTTTAAAC
3' CAAATTTG
5' ---GTTT AAAC--- 3'
3' ---CAAA TTTG--- 5'
MssI
Pme55I
Pseudomonas mendocina 55
5' AGGCCT
3' TCCGGA
5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5'
AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI
PmlI
Pseudomonas maltophilia
5' CACGTG
3' GTGCAC
5' ---CAC GTG--- 3'
3' ---GTG CAC--- 5'
AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PspCI
PovII
Proteus vulgaris
5' CAGCTG
3' GTCGAC
5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'
PpaAI
Phormidium papyraceum
5' TTCGAA
3' AAGCTT
5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5'
AsuII, BimI, Bsp119I, CbiI, Csp68KII, SfuI, SspRFI, SviI
PpaAII
Phormidium papyraceum
5' TCGA
3' AGCT
5' ---T CGA--- 3'
3' ---AGC T--- 5'
PpeI
Phormidium persicinum
5' GGGCCC
3' CCCGGG
5' ---GGGCC C--- 3'
3' ---C CCGGG--- 5'
ApaI, Bsp120I , PspOMI
PpiI
Pseudomonas putida Jo 4-731
5' GAACN5 CTC
3' CTTGN5 GAG
5' ---GAACN5 CTCN7 NNNNNN --- 3'
3' ---CTTGN5 GAGN7 N NNNNN--- 5'
PpsI
Pseudomonas pseudoalcaligenes PL
5' GAGTC
3' CTCAG
5' ---GAGTCNNNN N--- 3'
3' ---CTCAGNNNNN --- 5'
Ppu10I
Pseudomonas putida RFL10
5' ATGCAT
3' TACGTA
5' ---A TGCAT--- 3'
3' ---TACGT A--- 5'
BfrBI , Csp68KII , EcoT22I , Mph1103I , NsiI , SepI , SspD5II
Ppu111I
Pseudomonas putida P111
5' GAATTC
3' CTTAAG
5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'
PpuAI
Pseudomonas putida A1
5' CGTACG
3' GCATGC
5' ---C GTACG--- 3'
3' ---GCATG C--- 5'
PpuMI
Pseudomonas putida M
5' RGGWCCY
3' YCCWGGR
5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'
PpuXI
Pseudomonas putida X
5' RGGWCCY
3' YCCWGGR
5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'
PshAI
Plesiomonas shigelloides 319-73
5' GACN4 GTC
3' CTGN4 CAG
5' ---GACNN NNGTC--- 3'
3' ---CTGNN NNCAG--- 5'
PshBI
Plesiomonas shigelloides TPS970
5' ATTAAT
3' TAATTA
5' ---AT TAAT--- 3'
3' ---TAAT TA--- 5'
AseI, AsnI, BpoAI, Sru4DI, VspI
PsiI
Pseudomonas sp. SE-G49
5' TTATAA
3' AATATT
5' ---TTA TAA--- 3'
3' ---AAT ATT--- 5'
Psp03I
Phormidium sp.
5' GGWCC
3' CCWGG
5' ---GGWC C--- 3'
3' ---C CWGG--- 5'
Bme216I , CauI , EagMI , FdiI , HgiBI , HgiHIII , SinI , VpaK11AI
Psp5II
Pseudomonas sp. RFL5
5' RGGWCCY
3' YCCWGGR
5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'
Psp6I
Pseudomonas sp. B6
5' CCWGG
3' GGWCC
5' --- CCWGG--- 3'
3' ---GGWCC --- 5'
AeuI , AglI , ApaORI , Bse17I , EcoRII , Fsp1604I , SspAI, Sth117I
Psp23I
Pseudomonas sp. 23
5' CTGCAG
3' GACGTC
5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5'
ApiI, BspBI, CflI, MhaAI, Pfl21I, PstI, SalPI, Sst12I, YenI
Psp1406I
Pseudomonas pseudoalcaligenes RFL1406
5' AACGTT
3' TTGCAA
5' ---AA CGTT--- 3'
3' ---TTGC AA--- 5'
AclI
PspAI
Pseudomonas sp.
5' CCCGGG
3' GGGCCC
5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5'
AhyI, CfrJ4I , EaeAI, EclRI, Pac25I, SmaI , TspMI, XcyI, XmaI, XmaCI
PspALI
Pseudomonas sp. AL1637
5' CCCGGG
3' GGGCCC
5' ---CCC GGG--- 3'
3' ---GGG CCC--- 5'
AhyI, Cfr9I, EaeAI, EclRI, PspALI , SmaI , TspMI, XcyI, XmaI, XmaCI
Psp124BI
Pseudomonas sp. 124B
5' GAGCTC
3' CTCGAG
5' ---GAGCT C--- 3'
3' ---C TCGAG--- 5'
PspCI
Pseudomonas sp. C
5' CACGTG
3' GTGCAC
5' ---CAC GTG--- 3'
3' ---GTG CAC--- 5'
AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PmlI
PspEI
Pseudomonas sp. E
5' GGTNACC
3' CCANTGG
5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5'
AcrII, AspAI, BstEII, BstPI, Eci125I, Eco91I, EcoO65I, EcoO128I
PspGI
3BM3
Pyrococcus sp. GI-H
5' CCWGG
3' GGWCC
5' --- CCWGG--- 3'
3' ---GGWCC --- 5'
AeuI , AjnI, AorI , Bse17I , EcoRII , Fsp1604I , Psp6I, SspAI, Sth117I
PspLI
Pseudomonas sp. L
5' CGTACG
3' GCATGC
5' ---C GTACG--- 3'
3' ---GCATG C--- 5'
PspN4I
Pseudomonas sp. N4
5' GGNNCC
3' CCNNGG
5' ---GGN NCC--- 3'
3' ---CCN NGG--- 5'
AspNI, BscBI,
BmiI,
BspLI,
NlaIV
PspOMI
Pseudomonas sp. OM2164
5' GGGCCC
3' CCCGGG
5' ---G GGCCC--- 3'
3' ---CCCGG G--- 5'
ApaI , Bsp120I, PpeI
PspPI
Psychrobacter immobilis TA137
5' GGNCC
3' CCNGG
5' ---G GNCC--- 3'
3' ---CCNG G--- 5'
AsuI, Bal228I, BavBII, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96I
PspPPI
Pseudomonas sp. PP
5' RGGWCCY
3' YCCWGGR
5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'
PspXI
Pseudomonas sp. A1-1
5' VCTCGAGB
3' BGAGCTCV
5' ---VC TCGAGB--- 3'
3' ---BGAGCT CV--- 5'
PsrI
Pseudomonas stutzeri N2
5' GAACN6 TAC
3' CTTGN6 ATG
5' ---GAACN6 TACN6 NNNNNN --- 3'
3' ---CTTGN6 ATGN6 N NNNNN--- 5'
PssI
Pseudomonas sp.
5' RGGNCCY
3' YCCNGGR
5' ---RGGNC CY--- 3'
3' ---YC CNGGR--- 5'
PstI [ 7]
Providencia stuartii 164
5' CTGCAG
3' GACGTC
5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5'
AliAJI, BspBI, CfuII, Ecl2zI, HalII, PstI, Sag16I, Sag23I, Sst12I, XcpI
PstNHI
Pseudomonas stutzeri NH
5' GCTAGC
3' CGATCG
5' ---G CTAGC--- 3'
3' ---CGATC G--- 5'
AceII , AsuNHI, BmtI ,
BspOI ,
NheI, LlaG2I
PsuI
Pseudomonas stutzeri Mck 28-pH52
5' RGATCY
3' YCTAGR
5' ---R GATCY--- 3'
3' ---YCTAG R--- 5'
Psu161I
Pseudomonas stutzeri 161
5' CGATCG
3' GCTAGC
5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5'
Afa16RI, BspCI, EagBI, MvrI, Ple19I, PvuI, RshI, XorII
PsuAI
Phormidium subfuscum
5' YACGTR
3' RTGCAY
5' ---YAC GTR--- 3'
3' ---RTG CAY--- 5'
PsyI
Pseudomonas syringae Lki 1-pH124
5' GACNNNGTC
3' CTGNNNCAG
5' ---GACN NNGTC--- 3'
3' ---CTGNN NCAG--- 5'
AspI, AtsI, PflFI, TelI, Tth111I
PtaI
Phormidium tadzschicicum
5' TCCGGA
3' AGGCCT
5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5'
Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Kpn2I, PinBII, PtaI
Pun14627I
Phormidium uncinatum 1462/7
5' TGCGCA
3' ACGCGT
5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5'
Acc16I, AosI, AviII, FdiII, FspI, MstI, NsbI, PamI
Pun14627II
Phormidium uncinatum 1462/7
5' CAGCTG
3' GTCGAC
5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'
PunAI
Phormidium uncinatum
5' CYCGRG
3' GRGCYC
5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5'
AquI, BcoI, BsiHKCI, BspLU4I, Eco27kI, Nli3877I , PlaAI
PunAII
Phormidium uncinatum
5' RCATGY
3' YGTACR
5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'
PvuI
Proteus vulgaris
5' CGATCG
3' GCTAGC
5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5'
Afa22MI, BspCI, ErhB9I, NblI, Ple19I, Psu161I, RshI, XorII
PvuII
1PVU
Proteus vulgaris
5' CAGCTG
3' GTCGAC
5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'
Pvu84II
Proteus vulgaris 84
5' CAGCTG
3' GTCGAC
5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'
R
Enzyme
PDB code
Source
Recognition sequence
Cut
Isoschizomers
RalF40I
Ruminococcus albus F-40
5' GATC
3' CTAG
5' --- GATC--- 3'
3' ---CTAG --- 5'
BscFI, BspAI, BstENII, ChaI , HacI, MkrAI, Sau3AI, SsiAI
RcaI
Rhodococcus capsulatum
5' TCATGA
3' AGTACT
5' ---T CATGA--- 3'
3' ---AGTAC T--- 5'
RflFI
Ruminococcus flavefaciens FD-1
5' GTCGAC
3' CAGCTG
5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'
RflFII
Ruminococcus flavefaciens FD-1
5' AGTACT
3' TCATGA
5' ---AGT ACT--- 3'
3' ---TCA TGA--- 5'
Acc113I, AssI,
BmcAI, Bpa34I,
DpaI, Eco255I, ScaI, ZrmI
RleAI
Rhizobium leguminosarum
5' CCCACA
3' GGGTGT
5' ---CCCACAN8 NNNN --- 3'
3' ---GGGTGTN8 N NNN--- 5'
RmaI
Rhodothermus marinus
5' CTAG
3' GATC
5' ---C TAG--- 3'
3' ---GAT C--- 5'
Rme21I
Rhizobium meliloti
5' ATCGAT
3' TAGCTA
5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5'
BanIII, BbvAII, BliRI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I
RsaI
Rhodopseudomonas sphaeroides
5' GTAC
3' CATG
5' ---GT AC--- 3'
3' ---CA TG--- 5'
AfaI, Csp6I , CviQI , CviRII ,
HpyBI, PabI , PlaAII,
RsaNI ,
RshI
Rhodopseudomonas sphaeroides 2.4.1
5' CGATCG
3' GCTAGC
5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5'
Afa16RI, BspCI, EagBI, MvrI, Ple19I, Psu161I, PvuI, XorII
RspLKI
Rhodococcus sp. LK2
5' GCATGC
3' CGTACG
5' ---GCATG C--- 3'
3' ---C GTACG--- 5'
RspLKII
Rhodococcus sp. LK2
5' GGATCC
3' CCTAGG
5' ---G GATCC--- 3'
3' ---CCTAG G--- 5'
AliI, AsiI, BamHI , Bce751I, Nsp29132II, OkrAI, RspLKII, SolI
RspXI
Rhodococcus sp.
5' TCATGA
3' AGTACT
5' ---T CATGA--- 3'
3' ---AGTAC T--- 5'
RsrI
Rhodopseudomonas sphaeroides
5' GAATTC
3' CTTAAG
5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'
RsrII
Rhodopseudomonas sphaeroides
5' CGGWCCG
3' GCCWGGC
5' ---CG GWCCG--- 3'
3' ---GCCWG GC--- 5'
Rsr2I
Rhodobacter sphaeroides SE-I2
5' CGGWCCG
3' GCCWGGC
5' ---CG GWCCG--- 3'
3' ---GCCWG GC--- 5'
RtrI
Rhizobium trifolii
5' GTCGAC
3' CAGCTG
5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'
Rtr63I
Rhizobium trifolii 63
5' GTCGAC
3' CAGCTG
5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'
Notes
^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol . 81 (3): 419–23. doi :10.1016/0022-2836(73)90152-6 . PMID 4588280 .
^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes" . Nucleic Acids Res . 31 (7): 1805–12. doi :10.1093/nar/gkg274 . PMC 152790 . PMID 12654995 .
^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function" . Biochemistry . San Francisco: W. H. Freeman. ISBN 0-7167-4684-0 .
^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science . 181 (4096): 223–30. doi :10.1126/science.181.4096.223 . PMID 4124164 .
^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene . 92 (1–2): 1–248. doi :10.1016/0378-1119(90)90486-B . PMID 2172084 .
^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases" . Nucleic Acids Res . 29 (18): 3705–27. doi :10.1093/nar/29.18.3705 . PMC 55916 . PMID 11557805 .
^ Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3 .
Basic Concept Recognition sequence 4bp Recognition sequence 5bp Recognition sequence 6bp Recognition sequence 8bp Lists