List of restriction enzyme cutting sites: O–R

Main articles: List of restriction enzyme cutting sites and Restriction enzyme
Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
N A, C, G or T
M A or C
R A or G
W A or T
Y C or T
S C or G
K G or T
H A, C or T
B C, G or T
V A, C or G
D A, G or T

This article contains a list of the most studied restriction enzymes whose names start with O to R inclusive. It contains approximately 130 enzymes. The following information is given:

  • Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature[1][2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
  • PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action[3][4].
  • Source: Organism that naturally produces the enzyme.
  • Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
  • Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site[5][6].
  • Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:
as in this not listed enzyme:  EcoR70I 

Whole list navigation

Restriction enzymes

O

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
OfoI Oscillatoria foreaui A-1340 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, AvaI, Bse15I, BspLU4I, Eco27kI, Nli3877I, PlaAI, PunAI
OkrAI Oceanospirillum kriegii 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		BamHI, Bce751I, BnaI, Bsp98I, BstI, OkrAI, SolI, Uba4009I
OliI Oceanospirillum linum 4-5D 5' CACN4GTG
3' GTGN4CAC 		5' ---CACNN   NNGTG--- 3'
3' ---GTGNN   NNCAC--- 5' 		AleI
OxaNI Oerskovia xanthineolytica N 5' CCTNAGG
3' GGANTCC 		5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5' 		AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

P

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
PabI 2DVY Pyrococcus abyssi 5' GTAC
3' CATG 		5' ---GTA   C--- 3'
3' ---C   ATG--- 5'
AfaI, Csp6I, CviQI, HpyBI,
HpyBI, PlaAII, RsaI,  RsaNI, 
PacI Pseudomonas alcaligenes 5' TTAATTAA
3' AATTAATT 		5' ---TTAAT   TAA--- 3'
3' ---AAT   TAATT--- 5'
Pac25I Pseudomonas alcaligenes 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		AhyI, Cfr9I, EaeAI, EclRI, PspAI, PspALI, XcyI, XmaI, XmaCI
PaeI Pseudomonas aeruginosa 5' GCATGC
3' CGTACG 		5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
PaeAI Pseudomonas aeruginosa 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
PaeBI Pseudomonas aeruginosa - 18 5' CCCGGG
3' GGGCCC 		5' ---CCC   GGG--- 3'
3' ---GGG   CCC--- 5' 		AhyI, Cfr9I, EaeAI, EclRI, PspALI, SmaI, TspMI, XcyI, XmaI, XmaCI
PaeHI Pseudomonas aeruginosa 4148 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
PaeHII Pseudomonas aeruginosa 4148 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AliAJI, BspBI, BspMAI, CfuII, Ecl2zI, HalII, Srl5DI, Sst12I, XcpI
PaeQI Pseudomonas aeruginosa Q2 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
PaeR7I Pseudomonas aeruginosa PA0303 pMG7 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI
Pae2kI Pseudomonas aeruginosa 2k 5' AGATCT
3' TCTAGA 		5' ---A   GATCT--- 3'
3' ---TCTAG   A--- 5'
Pae5kI Pseudomonas aeruginosa 5k 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Pae14kI Pseudomonas aeruginosa 14k 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Pae17kI Pseudomonas aeruginosa 17k 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
Pae18kI Pseudomonas aeruginosa 18k 5' AGATCT
3' TCTAGA 		5' ---A   GATCT--- 3'
3' ---TCTAG   A--- 5'
PagI Pseudomonas alcaligenes Sau 14-027 5' TCATGA
3' AGTACT 		5' ---T   CATGA--- 3'
3' ---AGTAC   T--- 5'
PalI Providencia alcalifaciens 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
PamI Phormidium ambiguum 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AosI, AviII, FdiII, FspI, MstI, NsbI, Pun14627I
PamII Phormidium ambiguum 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AsuIII, BsaHI, BstACI, HgiDI, HgiGI, HgiHII, Hin1I, Hsp92I, Msp17I
PanI Pseudomonas alkanolytica 5' CTCGAG
3' GAGCTC 		5' ---C   TCGAG--- 3'
3' ---GAGCT   C--- 5' 		BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI
PasI Pseudomonas anguilliseptica RFL1 5' CCCWGGG
3' GGGWCCC 		5' ---CC   CWGGG--- 3'
3' ---GGGWC   CC--- 5' 		 — None on May 2010 —
PauI Paracoccus alcaliphilus ZVK3-3 5' GCGCGC
3' CGCGCG 		5' ---G   CGCGC--- 3'
3' ---CGCGC   G--- 5'
PauAI Phormidium autumnale 5' RCATGY
3' YGTACR 		5' ---RCATG   Y--- 3'
3' ---Y   GTACR--- 5'
PauAII Phormidium autumnale 5' TTTAAA
3' AAATTT 		5' ---TTT   AAA--- 3'
3' ---AAA   TTT--- 5' 		AhaIII, DraI, SruI
PceI Planococcus citreus 55 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI
PciI Planococcus citreus SE-F45 5' ACATGT
3' TGTACA 		5' ---A   CATGT--- 3'
3' ---TGTAC   A--- 5'
PctI Planococcus citreus SM 5' GAATGC
3' CTTACG 		5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp26HI, Asp27HI, Asp35HI
BmaHI, BsaMI, BscCI, Mva1269I
Pde12I Paracoccus denitrificans 12 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbI
Pde133I Paracoccus denitrificans 133 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
Pde137I Paracoccus denitrificans 137 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
PdiI Pseudomonas diminuta Mck 33-321 5' GCCGGC
3' CGGCCG 		5' ---GCC   GGC--- 3'
3' ---CGG   CCG--- 5'
PdmI Pseudomonas diminuta Auk 5-324 5' GAAN4TTC
3' CTTN4AAG 		5' ---GAANN   NNTTC--- 3'
3' ---CTTNN   NNAAG--- 5' 		Asp700I, BbvAI, MroXI, XmnI
PfaAI Phormidium favosum 5' GGYRCC
3' CCRYGG 		5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5' 		AccB1I, BanI, BbvBI, BshNI, Eco64I, HgiCI, HgiHI, MspB4I
PfaAII Phormidium favosum 5' CATATG
3' GTATAC 		5' ---CA   TATG--- 3'
3' ---GTAT   AC--- 5'
PfaAIII Phormidium favosum 5' GCATGC
3' CGTACG 		5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
PfeI Psychrobacter faecalis RFL1 5' GAWTC
3' CTWAG 		5' ---G   AWTC--- 3'
3' ---CTWA   G--- 5'
Pfl8I Pseudomonas fluorescens 8 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AliAJI, BspBI, BsuBI, CflI, CfrA4I, PaePI, Pfl21I, PstI, SflII, Sst12I
Pfl21I Pseudomonas sp. 21 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5'
Pfl23II Pseudomonas fluorescens RFL23 5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
Pfl27I Pseudomonas fluorescens RFL27 5' RGGWCCY
3' YCCWGGR 		5' ---RG   GWCCY--- 3'
3' ---YCCWG   GR--- 5'
PflBI Pseudomonas fluorescens type B 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflMI, Van91I
PflFI Pseudomonas fluorescens biotype F 5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AspI, AtsI, PsyI, TelI, Tth111I
PflKI Pseudomonas fluorescens 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
PflMI Pseudomonas fluorescens 5' CCAN5TGG
3' GGTN5ACC 		5' ---CCANNNN   NTGG--- 3'
3' ---GGTN   NNNNACC--- 5' 		AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, Van91I
PfoI Pseudomonas fluorescens biovar 126 5' TCCNGGA
3' AGGNCCT 		5' ---T   CCNGGA--- 3'
3' ---AGGNCC   T--- 5'
PgaI Pseudomonas gladioli B4 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BbvAII, BliAI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I
PhaI Pasteurella haemolytica 5' GCATC
3' CGTAG 		5' ---GCATCN4N   NNNN--- 3'
3' ---CGTAGN4NNNNN   --- 5'
PhoI Pyrococcus horikoshii OT3 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
PinAI Pseudomonas inequalis 5' ACCGGT
3' TGGCCA 		5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5'
AgeI, AsiAI,  AsiGI,  BshTI
CsiAI, CspAI
PinBI Phormidium inundatum 5' ATGCAT
3' TACGTA 		5' ---ATGCA   T--- 3'
3' ---T   ACGTA--- 5' 		EcoT22I, BfrBI, Mph1103I, NsiI, Ppu10I, SepI, SspD5II, Zsp2I
PinBII Phormidium inundatum 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		Aor13HI, BlfI, Bsp13I, BspEI, Bsu23I, Kpn2I, PinBII, PtaI
PlaI Phormidium lapideum 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
PlaII Phormidium lapideum 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, SfuI, SspRFI
PlaAI Phormidium laminosum 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, BcoI, Bse15I, BspLU4I, Eco27kI, Nli3877I, OfoI, PunAI
PlaAII Phormidium laminosum 5' GTAC
3' CATG 		5' ---GT   AC--- 3'
3' ---CA   TG--- 5'
AfaI, Csp6I, CviQI, CviRII,
HpyBI, PabI, RsaI,  RsaNI, 
PleI Pseudomonas lemoignei 5' GAGTC
3' CTCAG 		5' ---GAGTCNNNN   N--- 3'
3' ---CTCAGNNNNN   --- 5'
Ple19I Pseudomonas lemoignei 19 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa22MI, BspCI, ErhB9I, MvrI, Psu161I, PvuI, RshI, XorII
PmaCI Pseudomonas maltophilia CB50P 5' CACGTG
3' GTGCAC 		5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5' 		AcvI, BcoAI, BbrPI, Eco72I, PmlI, PspCI
PmeI Pseudomonas mendocina 5' GTTTAAAC
3' CAAATTTG 		5' ---GTTT   AAAC--- 3'
3' ---CAAA   TTTG--- 5' 		MssI
Pme55I Pseudomonas mendocina 55 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI
PmlI Pseudomonas maltophilia 5' CACGTG
3' GTGCAC 		5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5' 		AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PspCI
PovII Proteus vulgaris 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
PpaAI Phormidium papyraceum 5' TTCGAA
3' AAGCTT 		5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5' 		AsuII, BimI, Bsp119I, CbiI, Csp68KII, SfuI, SspRFI, SviI
PpaAII Phormidium papyraceum 5' TCGA
3' AGCT 		5' ---T   CGA--- 3'
3' ---AGC   T--- 5'
PpeI Phormidium persicinum 5' GGGCCC
3' CCCGGG 		5' ---GGGCC   C--- 3'
3' ---C   CCGGG--- 5' 		ApaI, Bsp120I, PspOMI
PpiI Pseudomonas putida Jo 4-731 5' GAACN5CTC
3' CTTGN5GAG 		5' ---GAACN5CTCN7NNNNNN   --- 3'
3' ---CTTGN5GAGN7N   NNNNN--- 5'
PpsI Pseudomonas pseudoalcaligenes PL 5' GAGTC
3' CTCAG 		5' ---GAGTCNNNN   N--- 3'
3' ---CTCAGNNNNN   --- 5'
Ppu10I Pseudomonas putida RFL10 5' ATGCAT
3' TACGTA 		5' ---A   TGCAT--- 3'
3' ---TACGT   A--- 5' 		BfrBI, Csp68KII, EcoT22I, Mph1103I, NsiI, SepI, SspD5II
Ppu111I Pseudomonas putida P111 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
PpuAI Pseudomonas putida A1 5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
PpuMI Pseudomonas putida M 5' RGGWCCY
3' YCCWGGR 		5' ---RG   GWCCY--- 3'
3' ---YCCWG   GR--- 5'
PpuXI Pseudomonas putida X 5' RGGWCCY
3' YCCWGGR 		5' ---RG   GWCCY--- 3'
3' ---YCCWG   GR--- 5'
PshAI Plesiomonas shigelloides 319-73 5' GACN4GTC
3' CTGN4CAG 		5' ---GACNN   NNGTC--- 3'
3' ---CTGNN   NNCAG--- 5'
PshBI Plesiomonas shigelloides TPS970 5' ATTAAT
3' TAATTA 		5' ---AT   TAAT--- 3'
3' ---TAAT   TA--- 5' 		AseI, AsnI, BpoAI, Sru4DI, VspI
PsiI Pseudomonas sp. SE-G49 5' TTATAA
3' AATATT 		5' ---TTA   TAA--- 3'
3' ---AAT   ATT--- 5'
Psp03I Phormidium sp. 5' GGWCC
3' CCWGG 		5' ---GGWC   C--- 3'
3' ---C   CWGG--- 5' 		Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, SinI, VpaK11AI
Psp5II Pseudomonas sp. RFL5 5' RGGWCCY
3' YCCWGGR 		5' ---RG   GWCCY--- 3'
3' ---YCCWG   GR--- 5'
Psp6I Pseudomonas sp. B6 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AeuI, AglI, ApaORI, Bse17I, EcoRII, Fsp1604I, SspAI, Sth117I
Psp23I Pseudomonas sp. 23 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		ApiI, BspBI, CflI, MhaAI, Pfl21I, PstI, SalPI, Sst12I, YenI
Psp1406I Pseudomonas pseudoalcaligenes RFL1406 5' AACGTT
3' TTGCAA 		5' ---AA   CGTT--- 3'
3' ---TTGC   AA--- 5' 		AclI
PspAI Pseudomonas sp. 5' CCCGGG
3' GGGCCC 		5' ---C   CCGGG--- 3'
3' ---GGGCC   C--- 5' 		AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, SmaI, TspMI, XcyI, XmaI, XmaCI
PspALI Pseudomonas sp. AL1637 5' CCCGGG
3' GGGCCC 		5' ---CCC   GGG--- 3'
3' ---GGG   CCC--- 5' 		AhyI, Cfr9I, EaeAI, EclRI, PspALI, SmaI, TspMI, XcyI, XmaI, XmaCI
Psp124BI Pseudomonas sp. 124B 5' GAGCTC
3' CTCGAG 		5' ---GAGCT   C--- 3'
3' ---C   TCGAG--- 5'
PspCI Pseudomonas sp. C 5' CACGTG
3' GTGCAC 		5' ---CAC   GTG--- 3'
3' ---GTG   CAC--- 5' 		AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PmlI
PspEI Pseudomonas sp. E 5' GGTNACC
3' CCANTGG 		5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5' 		AcrII, AspAI, BstEII, BstPI, Eci125I, Eco91I, EcoO65I, EcoO128I
PspGI 3BM3 Pyrococcus sp. GI-H 5' CCWGG
3' GGWCC 		5' ---   CCWGG--- 3'
3' ---GGWCC   --- 5' 		AeuI, AjnI, AorI, Bse17I, EcoRII, Fsp1604I, Psp6I, SspAI, Sth117I
PspLI Pseudomonas sp. L 5' CGTACG
3' GCATGC 		5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
PspN4I Pseudomonas sp. N4 5' GGNNCC
3' CCNNGG 		5' ---GGN   NCC--- 3'
3' ---CCN   NGG--- 5'
AspNI, BscBI,   BmiI,  BspLI,
NlaIV
PspOMI Pseudomonas sp. OM2164 5' GGGCCC
3' CCCGGG 		5' ---G   GGCCC--- 3'
3' ---CCCGG   G--- 5' 		ApaI, Bsp120I, PpeI
PspPI Psychrobacter immobilis TA137 5' GGNCC
3' CCNGG 		5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5' 		AsuI, Bal228I, BavBII, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96I
PspPPI Pseudomonas sp. PP 5' RGGWCCY
3' YCCWGGR 		5' ---RG   GWCCY--- 3'
3' ---YCCWG   GR--- 5'
PspXI Pseudomonas sp. A1-1 5' VCTCGAGB
3' BGAGCTCV 		5' ---VC   TCGAGB--- 3'
3' ---BGAGCT   CV--- 5'
PsrI Pseudomonas stutzeri N2 5' GAACN6TAC
3' CTTGN6ATG 		5' ---GAACN6TACN6NNNNNN   --- 3'
3' ---CTTGN6ATGN6N   NNNNN--- 5'
PssI Pseudomonas sp. 5' RGGNCCY
3' YCCNGGR 		5' ---RGGNC   CY--- 3'
3' ---YC   CNGGR--- 5'
PstI[7] Providencia stuartii 164 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AliAJI, BspBI, CfuII, Ecl2zI, HalII, PstI, Sag16I, Sag23I, Sst12I, XcpI
PstNHI Pseudomonas stutzeri NH 5' GCTAGC
3' CGATCG 		5' ---G   CTAGC--- 3'
3' ---CGATC   G--- 5'
AceII, AsuNHI, BmtI,  BspOI, 
NheI, LlaG2I
PsuI Pseudomonas stutzeri Mck 28-pH52 5' RGATCY
3' YCTAGR 		5' ---R   GATCY--- 3'
3' ---YCTAG   R--- 5'
Psu161I Pseudomonas stutzeri 161 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa16RI, BspCI, EagBI, MvrI, Ple19I, PvuI, RshI, XorII
PsuAI Phormidium subfuscum 5' YACGTR
3' RTGCAY 		5' ---YAC   GTR--- 3'
3' ---RTG   CAY--- 5'
PsyI Pseudomonas syringae Lki 1-pH124 5' GACNNNGTC
3' CTGNNNCAG 		5' ---GACN   NNGTC--- 3'
3' ---CTGNN   NCAG--- 5' 		AspI, AtsI, PflFI, TelI, Tth111I
PtaI Phormidium tadzschicicum 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Kpn2I, PinBII, PtaI
Pun14627I Phormidium uncinatum 1462/7 5' TGCGCA
3' ACGCGT 		5' ---TGC   GCA--- 3'
3' ---ACG   CGT--- 5' 		Acc16I, AosI, AviII, FdiII, FspI, MstI, NsbI, PamI
Pun14627II Phormidium uncinatum 1462/7 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
PunAI Phormidium uncinatum 5' CYCGRG
3' GRGCYC 		5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5' 		AquI, BcoI, BsiHKCI, BspLU4I, Eco27kI, Nli3877I, PlaAI
PunAII Phormidium uncinatum 5' RCATGY
3' YGTACR 		5' ---RCATG   Y--- 3'
3' ---Y   GTACR--- 5'
PvuI Proteus vulgaris 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa22MI, BspCI, ErhB9I, NblI, Ple19I, Psu161I, RshI, XorII
PvuII 1PVU Proteus vulgaris 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'
Pvu84II Proteus vulgaris 84 5' CAGCTG
3' GTCGAC 		5' ---CAG   CTG--- 3'
3' ---GTC   GAC--- 5'

R

Enzyme PDB code Source Recognition sequence Cut Isoschizomers
RalF40I Ruminococcus albus F-40 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		BscFI, BspAI, BstENII, ChaI, HacI, MkrAI, Sau3AI, SsiAI
RcaI Rhodococcus capsulatum 5' TCATGA
3' AGTACT 		5' ---T   CATGA--- 3'
3' ---AGTAC   T--- 5'
RflFI Ruminococcus flavefaciens FD-1 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
RflFII Ruminococcus flavefaciens FD-1 5' AGTACT
3' TCATGA 		5' ---AGT   ACT--- 3'
3' ---TCA   TGA--- 5'
Acc113I, AssI,  BmcAI, Bpa34I, 
DpaI, Eco255I, ScaI, ZrmI
RleAI Rhizobium leguminosarum 5' CCCACA
3' GGGTGT 		5' ---CCCACAN8NNNN   --- 3'
3' ---GGGTGTN8N   NNN--- 5'
RmaI Rhodothermus marinus 5' CTAG
3' GATC 		5' ---C   TAG--- 3'
3' ---GAT   C--- 5'
Rme21I Rhizobium meliloti 5' ATCGAT
3' TAGCTA 		5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5' 		BanIII, BbvAII, BliRI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I
RsaI Rhodopseudomonas sphaeroides 5' GTAC
3' CATG 		5' ---GT   AC--- 3'
3' ---CA   TG--- 5'
AfaI, Csp6I, CviQI, CviRII,
HpyBI, PabI, PlaAII,  RsaNI, 
RshI Rhodopseudomonas sphaeroides 2.4.1 5' CGATCG
3' GCTAGC 		5' ---CGAT   CG--- 3'
3' ---GC   TAGC--- 5' 		Afa16RI, BspCI, EagBI, MvrI, Ple19I, Psu161I, PvuI, XorII
RspLKI Rhodococcus sp. LK2 5' GCATGC
3' CGTACG 		5' ---GCATG   C--- 3'
3' ---C   GTACG--- 5'
RspLKII Rhodococcus sp. LK2 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5' 		AliI, AsiI, BamHI, Bce751I, Nsp29132II, OkrAI, RspLKII, SolI
RspXI Rhodococcus sp. 5' TCATGA
3' AGTACT 		5' ---T   CATGA--- 3'
3' ---AGTAC   T--- 5'
RsrI Rhodopseudomonas sphaeroides 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
RsrII Rhodopseudomonas sphaeroides 5' CGGWCCG
3' GCCWGGC 		5' ---CG   GWCCG--- 3'
3' ---GCCWG   GC--- 5'
Rsr2I Rhodobacter sphaeroides SE-I2 5' CGGWCCG
3' GCCWGGC 		5' ---CG   GWCCG--- 3'
3' ---GCCWG   GC--- 5'
RtrI Rhizobium trifolii 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
Rtr63I Rhizobium trifolii 63 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'

Notes

  1. ^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
  2. ^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
  3. ^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
  4. ^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
  5. ^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
  6. ^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
  7. ^ Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.