GlcA-beta-(1-2)-D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 4-beta-mannosyltransferase

GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 4-beta-mannosyltransferase
Identifiers
EC no.2.4.1.251
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GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 4-beta-mannosyltransferase (EC 2.4.1.251, GumI) is an enzyme with systematic name GDP-mannose:GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 4-beta-mannosyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol GDP + D-Man-beta-(1->4)- GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol

The enzyme is involved in the biosynthesis of the exopolysaccharide xanthan.

References

  1. ^ Katzen F, Ferreiro DU, Oddo CG, Ielmini MV, Becker A, Pühler A, Ielpi L (April 1998). "Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence". Journal of Bacteriology. 180 (7): 1607–17. PMC 107069. PMID 9537354.
  2. ^ Ielpi L, Couso RO, Dankert MA (May 1993). "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris". Journal of Bacteriology. 175 (9): 2490–500. PMC 204549. PMID 7683019.
  3. ^ Kim SY, Kim JG, Lee BM, Cho JY (February 2009). "Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae". Biotechnology Letters. 31 (2): 265–70. doi:10.1007/s10529-008-9858-3. PMID 18854951.