D-Man-alpha-(1-3)-D-Glc-beta-(1-4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase

D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
Identifiers
EC no.2.4.1.264
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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NCBIproteins

D-Man-alpha-(1->3) -D-Glc-beta-(1->4) -D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase (EC 2.4.1.264, GumK) is an enzyme with the systematic name UDP-glucuronate: D-Man-alpha-(1->3) -D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol beta-1,2-glucuronyltransferase.[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction:

UDP-glucuronate + D-Man-alpha-(1->3) -D-Glc-beta-(1->4) -D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol UDP + D-GlcA-beta-(1->2) -D-Man-alpha-(1->3) -D-Glc-beta-(1->4) -D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol

The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).

References

  1. ^ Katzen F, Ferreiro DU, Oddo CG, Ielmini MV, Becker A, Pühler A, Ielpi L (April 1998). "Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence". Journal of Bacteriology. 180 (7): 1607–17. doi:10.1128/JB.180.7.1607-1617.1998. PMC 107069. PMID 9537354.
  2. ^ Ielpi L, Couso RO, Dankert MA (May 1993). "Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris". Journal of Bacteriology. 175 (9): 2490–500. doi:10.1128/jb.175.9.2490-2500.1993. PMC 204549. PMID 7683019.
  3. ^ Kim SY, Kim JG, Lee BM, Cho JY (February 2009). "Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae". Biotechnology Letters. 31 (2): 265–70. doi:10.1007/s10529-008-9858-3. PMID 18854951.
  4. ^ Barreras M, Bianchet MA, Ielpi L (September 2006). "Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. 62 (Pt 9): 880–3. doi:10.1107/S1744309106028764. PMC 2242869. PMID 16946469.
  5. ^ Barreras M, Salinas SR, Abdian PL, Kampel MA, Ielpi L (September 2008). "Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase". The Journal of Biological Chemistry. 283 (36): 25027–35. doi:10.1074/jbc.M801227200. PMC 3259810. PMID 18596046.
  6. ^ Vojnov AA, Bassi DE, Daniels MJ, Dankert MA (February 2002). "Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris". Carbohydrate Research. 337 (4): 315–26. doi:10.1016/S0008-6215(01)00322-6. PMID 11841812.
  7. ^ Barreras M, Abdian PL, Ielpi L (March 2004). "Functional characterization of GumK, a membrane-associated beta-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis". Glycobiology. 14 (3): 233–41. doi:10.1093/glycob/cwh056. PMID 14736729.