The enzyme cyclohexyl-isocyanide hydratase (EC 4.2.1.103) catalyzes the chemical reaction

N-cyclohexylformamide ${\displaystyle \rightleftharpoons }$ cyclohexyl isocyanide + H₂O

This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is N-cyclohexylformamide hydro-lyase (cyclohexyl-isocyanide-forming). Other names in common use include isonitrile hydratase, and N-cyclohexylformamide hydro-lyase. This enzyme participates in caprolactam degradation.

• Goda M, Hashimoto Y, Shimizu S, Kobayashi M (2001). "Discovery of a novel enzyme, isonitrile hydratase, involved in nitrogen-carbon triple bond cleavage". J. Biol. Chem. 276 (26): 23480–5. doi:10.1074/jbc.M007856200. PMID 11306561.