23S rRNA (uridine2479-2'-O)-methyltransferase

23S rRNA (uridine2479-2'-O)-methyltransferase
Identifiers
EC no.2.1.1.208
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23S rRNA (uridine2479-2'-O)-methyltransferase (EC 2.1.1.208, AviRb) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (uridine2479-2'-O)-methyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + uridine2479 in 23S rRNA S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA

Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis.[4]

References

  1. ^ Mosbacher TG, Bechthold A, Schulz GE (January 2005). "Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes". Journal of Molecular Biology. 345 (3): 535–45. doi:10.1016/j.jmb.2004.10.051. PMID 15581897.
  2. ^ Treede I, Jakobsen L, Kirpekar F, Vester B, Weitnauer G, Bechthold A, Douthwaite S (July 2003). "The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose". Molecular Microbiology. 49 (2): 309–18. doi:10.1046/j.1365-2958.2003.03558.x. PMID 12828631.
  3. ^ Weitnauer G, Gaisser S, Trefzer A, Stockert S, Westrich L, Quiros LM, Mendez C, Salas JA, Bechthold A (March 2001). "An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tü57". Antimicrobial Agents and Chemotherapy. 45 (3): 690–5. doi:10.1128/aac.45.3.690-695.2001. PMC 90357. PMID 11181344.
  4. ^ Krupkin M, Wekselman I, Matzov D, Eyal Z, Diskin Posner Y, Rozenberg H, Zimmerman E, Bashan A, Yonath A (November 2016). "Avilamycin and evernimicin induce structural changes in rProteins uL16 and CTC that enhance the inhibition of A-site tRNA binding". Proceedings of the National Academy of Sciences of the United States of America. 113 (44): E6796 – E6805. doi:10.1073/pnas.1614297113. PMC 5098648. PMID 27791159.