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SLC26A2
Identifiers
Aliases	SLC26A2, D5S1708, DTD, DTDST, EDM4, MST153, MSTP157, solute carrier family 26 member 2
External IDs	OMIM: 606718; MGI: 892977; HomoloGene: 73876; GeneCards: SLC26A2; OMA:SLC26A2 - orthologs
Gene location (Human)
Chr.	Chromosome 5 (human)
End	149,993,455 bp
Gene location (Mouse)
Chr.	Chromosome 18 (mouse)
End	61,344,684 bp
RNA expression pattern
	Top expressed in
	mucosa of colon; ; mucosa of sigmoid colon; ; mucosa of transverse colon; ; rectum; ; seminal vesicula; ; mucosa of paranasal sinus; ; trigeminal ganglion; ; mucosa of ileum; ; skin of thigh; ; corpus epididymis;
	Top expressed in
	left colon; ; lacrimal gland; ; ileum; ; epithelium of stomach; ; salivary gland; ; parotid gland; ; transitional epithelium of urinary bladder; ; jejunum; ; rib; ; submandibular gland;
	More reference expression data
	n/a
Gene ontology
Molecular function	secondary active sulfate transmembrane transporter activity; bicarbonate transmembrane transporter activity; oxalate transmembrane transporter activity; chloride channel activity; sulfate transmembrane transporter activity; chloride transmembrane transporter activity;
Cellular component	integral component of membrane; membrane; extracellular exosome; integral component of plasma membrane; plasma membrane; apical plasma membrane; microvillus membrane;
Biological process	ossification; regulation of membrane potential; regulation of intracellular pH; chloride transmembrane transport; ion transport; bicarbonate transport; 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process; oxalate transport; transmembrane transport; sulfate transport; sulfate transmembrane transport; transport;
	Sources:Amigo / QuickGO
Orthologs
	1836
	13521
	ENSG00000155850
	ENSMUSG00000034320
	P50443
	Q62273
	NM_000112
	NM_007885
	NP_000103
	NP_031911
	Wikidata
View/Edit Human	View/Edit Mouse

The sulfate transporter is a solute carrier family protein that in humans is encoded by the SLC26A2 gene.^[5] SLC26A2 is also called the diastrophic dysplasia sulfate transporter (DTDST), and was first described by Hästbacka et al. in 1994.^[5] A defect in sulfate activation described by Superti-Furga in achondrogenesis type 1B^[6] was subsequently also found to be caused by genetic variants in the sulfate transporter gene.^[7] This sulfate (SO₄²⁻) transporter also accepts chloride, hydroxyl ions (OH⁻), and oxalate as substrates.^[8]^[9] SLC26A2 is expressed at high levels in developing and mature cartilage, as well as being expressed in lung, placenta, colon, kidney, pancreas and testis.^[10]^[11]

Function

The diastrophic dysplasia sulfate transporter is a transmembrane glycoprotein implicated in the pathogenesis of several human chondrodysplasias. In chondrocytes, SLC26A2 functions to transport most of the cellular sulfate, which is critical for the sulfation of proteoglycans and normal cartilage formation.^[12] In addition, studies have demonstrated that SLC26A2 influences chondrocyte proliferation, differentiation, and growth, suggesting that in the chondrocyte, SLC26A2 provides sulfate for both structural and regulatory proteins.^[13]

Clinical significance

Deficiencies are associated with many forms of osteochondrodysplasia.^[14]^[11] These include:

Correlation between genotype and phenotype

Since its first description, over 30 mutations in the SLC26A2 gene have been described in the four recessively inherited chondrodysplasias listed above. Achondrogenesis 1B (ACG-1B) is the most severe form of these chondrodysplasias, resulting in skeletal underdevelopment and death preceding or shortly after birth.^[15] Atelosteogenesis type II (AO-II) can be lethal in the neonatal period,^[16] whereas diastrophic dysplasia (DTD) and autosomal recessive multiple epiphyseal dysplasia (EDM4/rMED) are considered to be the least severe forms.

When ten previously described SLC26A2 mutation were expressed in mammalian cells, a strong correlation was found between the amount of sulfate transport activity of the mutated protein and the severity of the phenotype in patients where these mutations have been identified.^[17] For example, a mutation that results in a non-functional protein on both alleles was always found with the severe ACG-IB phenotype, but non-functional mutations on both alleles were never found with the less severe phenotypes, DTD and rMED. Mutations found in the moderately severe AO-II phenotype were always the result of a non-functioning mutation on one allele and a partial-functioning mutation on the opposite allele. In contrast, mutations described in the mildest form of the chondrodysplasia, rMED, result in proteins that retain at least some partial sulfate transport function on both alleles. This suggests that even a small amount of SLC26A2-mediated sulfate transport in chondrocytes can mitigate the clinical severity of the chondrodysplasia. However, a less predictable genotype/phenotype correlation has been found with a mutation described predominately in the Finnish population. This Finnish mutation is located in the splice site of the gene and results in low SLC26A2 mRNA levels.^[18] Different levels of expression of the SLC26A2 protein is probably the cause of the variable phenotypes described with this mutation.

Functional significance of SLC26A2 in the colon and the kidney

Immunohistochemical analysis has localized SLC26A2 to the apical membrane of colon epithelial cells and kidney proximal tubule cells.^[8]^[19]

Colon

Abundant SLC26A2 mRNA levels have been identified in the small and large intestine of mice, rats and humans. In the human colon, SLC26A2 is present in the upper third of the crypts, where it is directed toward the apical membrane.^[20] The physiological role of SLC26A2 in the human colon remains to be determined, but it likely represent the sulfate/oxalate exchanger that has been characterized in colonic apical membrane vesicle preparations and possibly plays an important role in sulfate transport in this tissue.^[21] In fact, impaired sulfation has been suggested to occur during the course of malignant transformation of colonic epithelial cells, and studies have shown that the growth rate of cancer cells was markedly enhanced when the transcription of SLC26A2 was suppressed.^[22]

Kidney

The SLC26A2 protein has been localized to the brush border membrane of the rat kidney proximal tubule.^[19] In that location, oxalate/SO₄²⁻ exchange, or chloride/SO₄²⁻ exchange by SLC26A2 might contribute to the critical process of sodium chloride reabsorption across the proximal tubular epithelium. Under one proposed model, an anion transporter exchanges intracellular oxalate for luminal chloride in parallel with the Na–SO₄ cotransporter, resulting in net sodium chloride readsorption.^[23] Under this model, a third transport process is required that functions as a method of recycling oxalate back into the cell, and recycling sulfate from the cell to the lumen. Previously, SLC26A6, another member of the same family of anion transporters as DTDST, was thought to provide the mechanism of oxalate- or formate-mediated chloride transport in this nephron segment; however, recent studies in Slc26a6-knockout mice have raised questions regarding its role in this transport process.^[24] In contrast, the apical membrane location, and electrochemical properties of SLC26A2 would fit the requirement of an anion exchanger located on the apical membrane of the proximal tubule that would serve as a mechanism of transporting chloride in exchange for oxalate, and/or recycling oxalate in exchange for sulfate.

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000155850 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000034320 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b Hästbacka J, de la Chapelle A, Mahtani MM, Clines G, Reeve-Daly MP, Daly M, Hamilton BA, Kusumi K, Trivedi B, Weaver A (September 1994). "The diastrophic dysplasia gene encodes a novel sulfate transporter: positional cloning by fine-structure linkage disequilibrium mapping". Cell. 78 (6): 1073–87. doi:10.1016/0092-8674(94)90281-X. PMID 7923357. S2CID 36181375.
^ Superti-Furga A (December 1994). "A defect in the metabolic activation of sulfate in a patient with achondrogenesis type IB". American Journal of Human Genetics. 55 (6): 1137–45. PMC 1918434. PMID 7977372.
^ Superti-Furga A, Hästbacka J, Wilcox WR, Cohn DH, van der Harten HJ, Rossi A, Blau N, Rimoin DL, Steinmann B, Lander ES, Gitzelmann R (January 1996). "Achondrogenesis type IB is caused by mutations in the diastrophic dysplasia sulphate transporter gene". Nature Genetics. 12 (1): 100–2. doi:10.1038/ng0196-100. PMID 8528239. S2CID 31143438.
^ ^a ^b Heneghan JF, Akhavein A, Salas MJ, Shmukler BE, Karniski LP, Vandorpe DH, Alper SL (June 2010). "Regulated transport of sulfate and oxalate by SLC26A2/DTDST". American Journal of Physiology. Cell Physiology. 298 (6): C1363-75. doi:10.1152/ajpcell.00004.2010. PMC 2889644. PMID 20219950.
^ Ohana E, Shcheynikov N, Park M, Muallem S (February 2012). "Solute carrier family 26 member a2 (Slc26a2) protein functions as an electroneutral SOFormula/OH-/Cl- exchanger regulated by extracellular Cl-". The Journal of Biological Chemistry. 287 (7): 5122–32. doi:10.1074/jbc.M111.297192. PMC 3281620. PMID 22190686.
^ Haila S, Hästbacka J, Böhling T, Karjalainen-Lindsberg ML, Kere J, Saarialho-Kere U (August 2001). "SLC26A2 (diastrophic dysplasia sulfate transporter) is expressed in developing and mature cartilage but also in other tissues and cell types". The Journal of Histochemistry and Cytochemistry. 49 (8): 973–82. doi:10.1177/002215540104900805. PMID 11457925.
^ ^a ^b Forlino A, Piazza R, Tiveron C, Della Torre S, Tatangelo L, Bonafè L, Gualeni B, Romano A, Pecora F, Superti-Furga A, Cetta G, Rossi A (March 2005). "A diastrophic dysplasia sulfate transporter (SLC26A2) mutant mouse: morphological and biochemical characterization of the resulting chondrodysplasia phenotype". Human Molecular Genetics. 14 (6): 859–71. doi:10.1093/hmg/ddi079. PMID 15703192.
^ "Entrez Gene: SLC26A2".
^ Park M, Ohana E, Choi SY, Lee MS, Park JH, Muallem S (January 2014). "Multiple roles of the SO4(2-)/Cl-/OH- exchanger protein Slc26a2 in chondrocyte functions". The Journal of Biological Chemistry. 289 (4): 1993–2001. doi:10.1074/jbc.M113.503466. PMC 3900949. PMID 24302720.
^ Superti-Furga A, Hästbacka J, Rossi A, van der Harten JJ, Wilcox WR, Cohn DH, Rimoin DL, Steinmann B, Lander ES, Gitzelmann R. A family of chondrodysplasias caused by mutations in the diastrophic dysplasia sulfate transporter gene and associated with impaired sulfation of proteoglycans. Ann N Y Acad Sci. 1996 Jun 8;785:195-201. doi: 10.1111/j.1749-6632.1996.tb56259.x. PMID: 8702127.
^ Superti-Furga A, Hästbacka J, Wilcox WR, Cohn DH, van der Harten HJ, Rossi A, Blau N, Rimoin DL, Steinmann B, Lander ES, Gitzelmann R (January 1996). "Achondrogenesis type IB is caused by mutations in the diastrophic dysplasia sulphate transporter gene". Nature Genetics. 12 (1): 100–2. doi:10.1038/ng0196-100. PMID 8528239. S2CID 31143438.
^ Hästbacka J, Superti-Furga A, Wilcox WR, Rimoin DL, Cohn DH, Lander ES (February 1996). "Atelosteogenesis type II is caused by mutations in the diastrophic dysplasia sulfate-transporter gene (DTDST): evidence for a phenotypic series involving three chondrodysplasias". American Journal of Human Genetics. 58 (2): 255–62. PMC 1914552. PMID 8571951.
^ Karniski LP (October 2004). "Functional expression and cellular distribution of diastrophic dysplasia sulfate transporter (DTDST) gene mutations in HEK cells". Human Molecular Genetics. 13 (19): 2165–71. doi:10.1093/hmg/ddh242. PMID 15294877.
^ Hästbacka J, Kerrebrock A, Mokkala K, Clines G, Lovett M, Kaitila I, de la Chapelle A, Lander ES (September 1999). "Identification of the Finnish founder mutation for diastrophic dysplasia (DTD)". European Journal of Human Genetics. 7 (6): 664–70. doi:10.1038/sj.ejhg.5200361. PMID 10482955.
^ ^a ^b Chapman JM, Karniski LP (May 2010). "Protein localization of SLC26A2 (DTDST) in rat kidney". Histochemistry and Cell Biology. 133 (5): 541–7. doi:10.1007/s00418-010-0694-x. PMID 20369363. S2CID 13591652.
^ Haila S, Saarialho-Kere U, Karjalainen-Lindsberg ML, Lohi H, Airola K, Holmberg C, Hästbacka J, Kere J, Höglund P (April 2000). "The congenital chloride diarrhea gene is expressed in seminal vesicle, sweat gland, inflammatory colon epithelium, and in some dysplastic colon cells". Histochemistry and Cell Biology. 113 (4): 279–86. doi:10.1007/s004180000131. PMID 10857479. S2CID 10999468.
^ Tyagi S, Kavilaveettil RJ, Alrefai WA, Alsafwah S, Ramaswamy K, Dudeja PK (November 2001). "Evidence for the existence of a distinct SO(4)(--)-OH(-) exchange mechanism in the human proximal colonic apical membrane vesicles and its possible role in chloride transport". Experimental Biology and Medicine. 226 (10): 912–8. doi:10.1177/153537020122601006. PMID 11682697. S2CID 24469074.
^ Yusa A, Miyazaki K, Kimura N, Izawa M, Kannagi R (May 2010). "Epigenetic silencing of the sulfate transporter gene DTDST induces sialyl Lewisx expression and accelerates proliferation of colon cancer cells". Cancer Research. 70 (10): 4064–73. doi:10.1158/0008-5472.CAN-09-2383. PMID 20460514.
^ Kuo SM, Aronson PS (June 1996). "Pathways for oxalate transport in rabbit renal microvillus membrane vesicles". The Journal of Biological Chemistry. 271 (26): 15491–7. doi:10.1074/jbc.271.26.15491. PMID 8663096.
^ Knauf F, Velazquez H, Pfann V, Jiang Z, Aronson PS (January 2019). "Characterization of renal NaCl and oxalate transport in Slc26a6 -/- mice". American Journal of Physiology. Renal Physiology. 316 (1): F128 – F133. doi:10.1152/ajprenal.00309.2018. PMC 6383200. PMID 30427220.

External links

SLC26A2+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000155850 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000034320 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid7923357-5] Hästbacka J, de la Chapelle A, Mahtani MM, Clines G, Reeve-Daly MP, Daly M, Hamilton BA, Kusumi K, Trivedi B, Weaver A (September 1994). "The diastrophic dysplasia gene encodes a novel sulfate transporter: positional cloning by fine-structure linkage disequilibrium mapping". Cell. 78 (6): 1073–87. doi:10.1016/0092-8674(94)90281-X. PMID 7923357. S2CID 36181375.

[pmid7977372-6] Superti-Furga A (December 1994). "A defect in the metabolic activation of sulfate in a patient with achondrogenesis type IB". American Journal of Human Genetics. 55 (6): 1137–45. PMC 1918434. PMID 7977372.

[pmid8528239-7] Superti-Furga A, Hästbacka J, Wilcox WR, Cohn DH, van der Harten HJ, Rossi A, Blau N, Rimoin DL, Steinmann B, Lander ES, Gitzelmann R (January 1996). "Achondrogenesis type IB is caused by mutations in the diastrophic dysplasia sulphate transporter gene". Nature Genetics. 12 (1): 100–2. doi:10.1038/ng0196-100. PMID 8528239. S2CID 31143438.

[:0-8] Heneghan JF, Akhavein A, Salas MJ, Shmukler BE, Karniski LP, Vandorpe DH, Alper SL (June 2010). "Regulated transport of sulfate and oxalate by SLC26A2/DTDST". American Journal of Physiology. Cell Physiology. 298 (6): C1363-75. doi:10.1152/ajpcell.00004.2010. PMC 2889644. PMID 20219950.

[9] Ohana E, Shcheynikov N, Park M, Muallem S (February 2012). "Solute carrier family 26 member a2 (Slc26a2) protein functions as an electroneutral SOFormula/OH-/Cl- exchanger regulated by extracellular Cl-". The Journal of Biological Chemistry. 287 (7): 5122–32. doi:10.1074/jbc.M111.297192. PMC 3281620. PMID 22190686.

[10] Haila S, Hästbacka J, Böhling T, Karjalainen-Lindsberg ML, Kere J, Saarialho-Kere U (August 2001). "SLC26A2 (diastrophic dysplasia sulfate transporter) is expressed in developing and mature cartilage but also in other tissues and cell types". The Journal of Histochemistry and Cytochemistry. 49 (8): 973–82. doi:10.1177/002215540104900805. PMID 11457925.

[pmid15703192-11] Forlino A, Piazza R, Tiveron C, Della Torre S, Tatangelo L, Bonafè L, Gualeni B, Romano A, Pecora F, Superti-Furga A, Cetta G, Rossi A (March 2005). "A diastrophic dysplasia sulfate transporter (SLC26A2) mutant mouse: morphological and biochemical characterization of the resulting chondrodysplasia phenotype". Human Molecular Genetics. 14 (6): 859–71. doi:10.1093/hmg/ddi079. PMID 15703192.

[entrez-12] "Entrez Gene: SLC26A2".

[13] Park M, Ohana E, Choi SY, Lee MS, Park JH, Muallem S (January 2014). "Multiple roles of the SO4(2-)/Cl-/OH- exchanger protein Slc26a2 in chondrocyte functions". The Journal of Biological Chemistry. 289 (4): 1993–2001. doi:10.1074/jbc.M113.503466. PMC 3900949. PMID 24302720.

[14] Superti-Furga A, Hästbacka J, Rossi A, van der Harten JJ, Wilcox WR, Cohn DH, Rimoin DL, Steinmann B, Lander ES, Gitzelmann R. A family of chondrodysplasias caused by mutations in the diastrophic dysplasia sulfate transporter gene and associated with impaired sulfation of proteoglycans. Ann N Y Acad Sci. 1996 Jun 8;785:195-201. doi: 10.1111/j.1749-6632.1996.tb56259.x. PMID: 8702127.

[15] Superti-Furga A, Hästbacka J, Wilcox WR, Cohn DH, van der Harten HJ, Rossi A, Blau N, Rimoin DL, Steinmann B, Lander ES, Gitzelmann R (January 1996). "Achondrogenesis type IB is caused by mutations in the diastrophic dysplasia sulphate transporter gene". Nature Genetics. 12 (1): 100–2. doi:10.1038/ng0196-100. PMID 8528239. S2CID 31143438.

[16] Hästbacka J, Superti-Furga A, Wilcox WR, Rimoin DL, Cohn DH, Lander ES (February 1996). "Atelosteogenesis type II is caused by mutations in the diastrophic dysplasia sulfate-transporter gene (DTDST): evidence for a phenotypic series involving three chondrodysplasias". American Journal of Human Genetics. 58 (2): 255–62. PMC 1914552. PMID 8571951.

[17] Karniski LP (October 2004). "Functional expression and cellular distribution of diastrophic dysplasia sulfate transporter (DTDST) gene mutations in HEK cells". Human Molecular Genetics. 13 (19): 2165–71. doi:10.1093/hmg/ddh242. PMID 15294877.

[18] Hästbacka J, Kerrebrock A, Mokkala K, Clines G, Lovett M, Kaitila I, de la Chapelle A, Lander ES (September 1999). "Identification of the Finnish founder mutation for diastrophic dysplasia (DTD)". European Journal of Human Genetics. 7 (6): 664–70. doi:10.1038/sj.ejhg.5200361. PMID 10482955.

[:1-19] Chapman JM, Karniski LP (May 2010). "Protein localization of SLC26A2 (DTDST) in rat kidney". Histochemistry and Cell Biology. 133 (5): 541–7. doi:10.1007/s00418-010-0694-x. PMID 20369363. S2CID 13591652.

[20] Haila S, Saarialho-Kere U, Karjalainen-Lindsberg ML, Lohi H, Airola K, Holmberg C, Hästbacka J, Kere J, Höglund P (April 2000). "The congenital chloride diarrhea gene is expressed in seminal vesicle, sweat gland, inflammatory colon epithelium, and in some dysplastic colon cells". Histochemistry and Cell Biology. 113 (4): 279–86. doi:10.1007/s004180000131. PMID 10857479. S2CID 10999468.

[21] Tyagi S, Kavilaveettil RJ, Alrefai WA, Alsafwah S, Ramaswamy K, Dudeja PK (November 2001). "Evidence for the existence of a distinct SO(4)(--)-OH(-) exchange mechanism in the human proximal colonic apical membrane vesicles and its possible role in chloride transport". Experimental Biology and Medicine. 226 (10): 912–8. doi:10.1177/153537020122601006. PMID 11682697. S2CID 24469074.

[22] Yusa A, Miyazaki K, Kimura N, Izawa M, Kannagi R (May 2010). "Epigenetic silencing of the sulfate transporter gene DTDST induces sialyl Lewisx expression and accelerates proliferation of colon cancer cells". Cancer Research. 70 (10): 4064–73. doi:10.1158/0008-5472.CAN-09-2383. PMID 20460514.

[23] Kuo SM, Aronson PS (June 1996). "Pathways for oxalate transport in rabbit renal microvillus membrane vesicles". The Journal of Biological Chemistry. 271 (26): 15491–7. doi:10.1074/jbc.271.26.15491. PMID 8663096.

[24] Knauf F, Velazquez H, Pfann V, Jiang Z, Aronson PS (January 2019). "Characterization of renal NaCl and oxalate transport in Slc26a6 -/- mice". American Journal of Physiology. Renal Physiology. 316 (1): F128 – F133. doi:10.1152/ajprenal.00309.2018. PMC 6383200. PMID 30427220.

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PROFILPELAJAR.COM

Sulfate transporter

Function

Clinical significance

Correlation between genotype and phenotype

Functional significance of SLC26A2 in the colon and the kidney

Colon

Kidney

References

Further reading

External links

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