Lactase

Not to be confused with Lactose.
Lactase
E. coli lactase tetramer. PDB: 1JYN​
Identifiers
EC no.3.2.1.108
CAS no.9031-11-2
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins
Lactase
Identifiers
SymbolLCT
Alt. symbolsLAC; LPH; LPH1
NCBI gene3938
HGNC6530
OMIM603202
RefSeqNM_002299
UniProtP09848
Other data
EC number3.2.1.108
LocusChr. 2 q21
Search for
StructuresSwiss-model
DomainsInterPro

Lactase (EC 3.2.1.108) is an enzyme produced by many organisms and is essential to the complete digestion of whole milk. It breaks down the sugar lactose into its component parts, galactose and glucose. Lactase is found in the brush border of the small intestine of humans and other mammals. People deficient in lactase or lacking functional lactase may experience the symptoms of lactose intolerance after consuming milk products.[1] Microbial β-galactosidase (often loosely referred to as lactase) can be purchased as a food supplement and is added to milk to produce "lactose-free" milk products.

Uses

Food use

Lactase is an enzyme that some people are unable to produce in their small intestine.[2] Technology to produce lactose-free milk, ice cream, and yogurt was developed by the USDA Agricultural Research Service in 1985.[3] This technology is used to add lactase to milk, thereby hydrolyzing the lactose naturally found in milk, leaving it slightly sweet but digestible by everyone.[4] Without lactase, lactose-intolerant people pass the lactose undigested to the colon[5] where bacteria break it down, creating carbon dioxide which leads to bloating and flatulence.

Medical use

Lactase supplements can be used to treat lactose intolerance.[6]

Industrial use

Lactase produced commercially can be extracted both from yeasts such as Kluyveromyces fragilis and Kluyveromyces lactis and from molds, such as Aspergillus niger and Aspergillus oryzae.[7] Its primary commercial use in supplements is to break down lactose in milk to make it suitable for people with lactose intolerance.[8][9] The U.S. Food and Drug Administration has not independently evaluated these products.[10]

Lactase (or a similar form of β-galactosidase) is also used to screen for blue white colonies in the multiple cloning sites of various plasmid vectors in Escherichia coli or other bacteria.[11][12]

Mechanism

The temperature optimum for human lactase is about 37 °C[13] and the pH optimum is 6.[14]

In metabolism, the β-glycosidic bond in D-lactose is hydrolyzed to form D-galactose and D-glucose, which can be absorbed through the intestinal walls and into the bloodstream. The overall reaction that lactase catalyzes is as follows:

C12H22O11 + H2O → C6H12O6 + C6H12O6 + heat.
lactose + H2O → β-D-galactose + D-glucose

The catalytic mechanism of D-lactose hydrolysis retains the substrate anomeric configuration in the products.[15] While the details of the mechanism are uncertain, the stereochemical retention is achieved off a double displacement reaction. Studies of E. coli lactase have proposed that hydrolysis is initiated when a glutamate nucleophile on the enzyme attacks from the axial side of the galactosyl carbon in the β-glycosidic bond.[16] The removal of the D-glucose leaving group may be facilitated by Mg-dependent acid catalysis.[16] The enzyme is liberated from the α-galactosyl moiety upon equatorial nucleophilic attack by water, which produces D-galactose.[15]

Substrate modification studies have demonstrated that the 3′-OH and 2′-OH moieties on the galactopyranose ring are essential for enzymatic recognition and hydrolysis.[17] The 3′-hydroxy group is involved in initial binding to the substrate while the 2′- group is not necessary for recognition but needed in subsequent steps. This is demonstrated by the fact that a 2-deoxy analog is an effective competitive inhibitor (Ki = 10mM).[17] Elimination of specific hydroxyl groups on the glucopyranose moiety does not eliminate catalysis.[17]

Proposed mechanism of lactose hydrolysis by Lactase enzyme

Lactase also catalyzes the conversion of phlorizin to phloretin and glucose.

Lactase (Lactaid commercially) is used as a medication for lactose intolerance. Since it is an enzyme, its function can be inhibited by the acidity of the stomach. However, it is packaged in an acid-proof tablet, allowing the enzyme to pass through the stomach intact and remain in the small intestine. In the small intestine it can act on ingested lactose molecules, allowing the body to absorb the digested sugar which would otherwise cause cramping and diarrhea. Since the enzyme is not absorbed, it is excreted during the next bowel movement.[citation needed]

Structure and biosynthesis

Preprolactase, the primary translation product, has a single polypeptide primary structure consisting of 1927 amino acids.[18] It can be divided into five domains: (i) a 19-amino-acid cleaved signal sequence; (ii) a large prosequence domain that is not present in mature lactase; (iii) the mature lactase segment; (iv) a membrane-spanning hydrophobic anchor; and (v) a short hydrophilic carboxyl terminus.[18] The signal sequence is cleaved in the endoplasmic reticulum, and the resulting 215-kDa pro-LPH is sent to the Golgi apparatus, where it is heavily glycosylated and proteolytically processed to its mature form.[19] The prodomain has been shown to act as an intramolecular chaperone in the ER, preventing trypsin cleavage and allowing LPH to adopt the necessary 3-D structure to be transported to the Golgi apparatus.[20]

Schematic of processing and localization of human lactase translational product

Mature human lactase consists of a single 160-kDa polypeptide chain that localizes to the brush border membrane of intestinal epithelial cells. It is oriented with the N-terminus outside the cell and the C-terminus in the cytosol.[18] LPH contains two catalytic glutamic acid sites. In the human enzyme, the lactase activity has been connected to Glu-1749, while Glu-1273 is the site of phlorizin hydrolase function.[21]

Genetic expression and regulation

In humans, lactase is encoded by a single genetic locus on chromosome 2.[22] It is expressed exclusively by mammalian small intestine enterocytes and in very low levels in the colon during fetal development.[22] Humans are born with high levels of lactase expression. In most of the world's population, lactase transcription is down-regulated after weaning, resulting in diminished lactase expression in the small intestine,[22] which causes the common symptoms of adult-type hypolactasia, or lactose intolerance.[23] The LCT gene provides the instructions for making lactase. Lactose intolerance in infants (congenital lactase deficiency) is caused by mutations in the LCT gene. Mutations are believed to interfere with the function of lactase, causing affected infants to have a severely impaired ability to digest lactose in breast milk or formula.[24]

Some population segments exhibit lactase persistence resulting from a mutation that is postulated to have occurred 5,000–10,000 years ago, coinciding with the rise of cattle domestication.[25] This mutation has allowed almost half of the world's population to metabolize lactose without symptoms. Studies have linked the occurrence of lactase persistence to two different single-nucleotide polymorphisms about 14 and 22 kilobases upstream of the 5'-end of the LPH gene.[26] Both mutations, C→T at position -13910 and G→ A at position -22018, have been independently linked to lactase persistence.[27]

The lactase promoter is 150 base pairs long and is located upstream of the site of transcription initiation.[27] The sequence is highly conserved in mammals, suggesting that critical cis-transcriptional regulators are located nearby.[27] Cdx-2, HNF-1α, and GATA have been identified as transcription factors.[27] Studies of hypolactasia onset have demonstrated that despite polymorphisms, little difference exists in lactase expression in infants, showing that the mutations become increasingly relevant during development.[28] Developmentally regulated DNA-binding proteins may down-regulate transcription or destabilize mRNA transcripts, causing decreased LPH expression after weaning.[28]

See also

References

  1. ^ Järvelä I, Torniainen S, Kolho KL (2009). "Molecular genetics of human lactase deficiencies". Annals of Medicine. 41 (8): 568–75. doi:10.1080/07853890903121033. PMID 19639477. S2CID 205586720.
  2. ^ "Lactose Intolerance". Mayo Clinic. Retrieved 13 March 2018.
  3. ^ Porch K (2018-04-12). "Lactose-Free Milk, Low-Fat Cheese, and More Dairy Breakthroughs". www.federallabs.org. Retrieved 2018-10-26.
  4. ^ "Asked: How do dairies make lactose-free milk?". USA Today. 3 September 2014. Retrieved 13 March 2018.
  5. ^ "Lactose intolerance - Symptoms and causes". Mayo Clinic. Retrieved 2020-11-08.
  6. ^ "Lactose Intolerance". NIDDK. June 2014. Retrieved 25 October 2016.
  7. ^ Seyis I, Aksoz N (2004). "Production of Lactase by Trichoderma sp". Food Technology and Biotechnology. 42 (2): 121–124.
  8. ^ DSM Food Specialties (3 April 2014). "GRAS Notification for Acid Lactase from Aspergillus oryzae Expressed in Aspergillus niger" (PDF). p. 1. Archived from the original on 31 October 2017 – via U.S. Food and Drug Administration.
  9. ^ Holsinger VH (1992). "Innovative Products for Food Industries: The Lactaid Story". New Crops, New Uses, New Markets: 1992 Yearbook of Agriculture. U.S. Department of Agriculture. pp. 256–258. Archived from the original on 2022-07-17. Retrieved 2022-01-11.
  10. ^ Tarantino, LM (12 December 2003). "Agency Response Letter GRAS Notice No. GRN 000132". U.S. Food and Drug Administration. Archived from the original on 26 March 2011.
  11. ^ Lau HM, Lee LS, Soh WC, Tue SW (March 2013). "Introduction". Lactase. Universiti Teknologi Malaysia. Retrieved 16 November 2018.
  12. ^ "pBluescript II KS(+/−), pBluescript II SK(+/−): description & restriction map". Fermentas. Archived from the original on 19 October 2008.
  13. ^ Hermida C, Corrales G, Cañada FJ, Aragón JJ, Fernández-Mayoralas A (Jul 2007). "Optimizing the enzymatic synthesis of β-D-galactopyranosyl-D-xyloses for their use in the evaluation of lactase activity in vivo". Bioorganic & Medicinal Chemistry. 15 (14): 4836–40. doi:10.1016/j.bmc.2007.04.067. hdl:10261/81580. PMID 17512743.
  14. ^ Skovbjerg H, Sjöström H, Norén O (Mar 1981). "Purification and characterisation of amphiphilic lactase/phlorizin hydrolase from human small intestine". European Journal of Biochemistry. 114 (3): 653–61. doi:10.1111/j.1432-1033.1981.tb05193.x. PMID 6786877.
  15. ^ a b Sinnott M (November 1990). "Catalytic mechanisms of enzymic glycosyl transfer". Chem. Rev. 90 (7): 1171–1202. doi:10.1021/cr00105a006.
  16. ^ a b Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW (Dec 2001). "A structural view of the action of Escherichia coli (lacZ) β-galactosidase". Biochemistry. 40 (49): 14781–94. doi:10.1021/bi011727i. PMID 11732897.
  17. ^ a b c Fernandez P, Cañada FJ, Jiménez-Barbero J, Martín-Lomas M (Jul 1995). "Substrate specificity of small-intestinal lactase: study of the steric effects and hydrogen bonds involved in enzyme-substrate interaction". Carbohydrate Research. 271 (1): 31–42. doi:10.1016/0008-6215(95)00034-Q. PMID 7648581.
  18. ^ a b c Mantei N, Villa M, Enzler T, Wacker H, Boll W, James P, Hunziker W, Semenza G (Sep 1988). "Complete primary structure of human and rabbit lactase-phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme". The EMBO Journal. 7 (9): 2705–13. doi:10.1002/j.1460-2075.1988.tb03124.x. PMC 457059. PMID 2460343.
  19. ^ Naim HY, Sterchi EE, Lentze MJ (Jan 1987). "Biosynthesis and maturation of lactase-phlorizin hydrolase in the human small intestinal epithelial cells". The Biochemical Journal. 241 (2): 427–34. doi:10.1042/bj2410427. PMC 1147578. PMID 3109375.
  20. ^ Naim HY, Jacob R, Naim H, Sambrook JF, Gething MJ (Oct 1994). "The pro region of human intestinal lactase-phlorizin hydrolase". The Journal of Biological Chemistry. 269 (43): 26933–43. doi:10.1016/S0021-9258(18)47109-8. PMID 7523415.
  21. ^ Zecca L, Mesonero JE, Stutz A, Poirée JC, Giudicelli J, Cursio R, Gloor SM, Semenza G (Sep 1998). "Intestinal lactase-phlorizin hydrolase (LPH): the two catalytic sites; the role of the pancreas in pro-LPH maturation". FEBS Letters. 435 (2–3): 225–8. Bibcode:1998FEBSL.435..225Z. doi:10.1016/S0014-5793(98)01076-X. PMID 9762914. S2CID 33421778.
  22. ^ a b c Troelsen JT, Mitchelmore C, Spodsberg N, Jensen AM, Norén O, Sjöström H (Mar 1997). "Regulation of lactase-phlorizin hydrolase gene expression by the caudal-related homoeodomain protein Cdx-2". The Biochemical Journal. 322 ( Pt 3) (Pt. 3): 833–8. doi:10.1042/bj3220833. PMC 1218263. PMID 9148757.
  23. ^ Reference GH. "LCT gene". Genetics Home Reference. Retrieved 3 April 2018.
  24. ^ "Lactose intolerance: MedlinePlus Genetics". medlineplus.gov. Retrieved 2022-03-22.
  25. ^ Bersaglieri T, Sabeti PC, Patterson N, Vanderploeg T, Schaffner SF, Drake JA, Rhodes M, Reich DE, Hirschhorn JN (Jun 2004). "Genetic signatures of strong recent positive selection at the lactase gene". American Journal of Human Genetics. 74 (6): 1111–20. doi:10.1086/421051. PMC 1182075. PMID 15114531.
  26. ^ Kuokkanen M, Enattah NS, Oksanen A, Savilahti E, Orpana A, Järvelä I (May 2003). "Transcriptional regulation of the lactase-phlorizin hydrolase gene by polymorphisms associated with adult-type hypolactasia". Gut. 52 (5): 647–52. doi:10.1136/gut.52.5.647. PMC 1773659. PMID 12692047.
  27. ^ a b c d Troelsen JT (May 2005). "Adult-type hypolactasia and regulation of lactase expression". Biochimica et Biophysica Acta (BBA) - General Subjects. 1723 (1–3): 19–32. doi:10.1016/j.bbagen.2005.02.003. PMID 15777735.
  28. ^ a b Wang Y, Harvey CB, Hollox EJ, Phillips AD, Poulter M, Clay P, Walker-Smith JA, Swallow DM (Jun 1998). "The genetically programmed down-regulation of lactase in children". Gastroenterology. 114 (6): 1230–6. doi:10.1016/S0016-5085(98)70429-9. PMID 9609760.
Wikimedia Commons has media related to Lactase.
  1. ^ Beebe JA, Frey PA (1998-10-01). "Galactose Mutarotase: Purification, Characterization, and Investigations of Two Important Histidine Residues". Biochemistry. 37 (42): 14989–14997. doi:10.1021/bi9816047. ISSN 0006-2960.

Read other articles:

Eugenika di Amerika Serikat

Keluarga pemenang kontes Keluarga Bugar berdiri di luar Gedung Eugenics[1] (di mana kontestan yang telah mendaftar) di Kansas Free Fair, di Topeka, KS. Eugenika merupakan seperangkat keyakinan dan praktik yang bertujuan untuk meningkatkan kualitas genetika suatu populasi penduduk dunia,[2][3] yang memainkan peran penting dalam sejarah dan budaya Amerika Serikat sebelum keterlibatannya dalam Perang Dunia II.[4] Eugenika telah dipraktikkan di Amerika Serikat sela...

 

 

Molló

Municipality in Catalonia, SpainMollóMunicipalitySt. Cecilia's church, Molló (12th century) FlagCoat of armsMollóLocation in CataloniaShow map of Province of GironaMollóMolló (Spain)Show map of SpainCoordinates: 42°21′N 2°24′E / 42.350°N 2.400°E / 42.350; 2.400Country SpainCommunity CataloniaProvince GironaComarcaRipollèsGovernment • MayorJosep Coma Guitart (2015)[1]Area[2] • Total43.1 km2 (16.6&...

 

 

Hakeem Jeffries

Hakeem Jeffries Hakeem Sekou Jeffries (/ˌhɑːˈkiːm/; lahir 4 Agustus 1970)[1] adalah seorang politikus dan jaksa Amerika Serikat yang menjabat sebagai anggota DPR sejak 2013. Ia adalah anggota Partai Demokrat. Referensi ^ Hakeem Sekou Jeffries – New York – Bio, News, Photos. Washington Times. October 12, 2012. Diarsipkan dari versi asli tanggal September 27, 2013. Diakses tanggal September 28, 2013.  Parameter |url-status= yang tidak diketahui akan diabaikan (bantuan...

Kohila

Borough in Estonia Borough in Rapla County, EstoniaKohilaBoroughKohila manorKohilaLocation in EstoniaCoordinates: 59°10′4″N 24°45′1″E / 59.16778°N 24.75028°E / 59.16778; 24.75028Country EstoniaCounty Rapla CountyMunicipality Kohila ParishPopulation (01.01.2006) • Total3,505 Drone video of Kohila bridges, watermill and town (June 2022) Kohila (formerly also Kapa-Kohila, German: Koil, Kappakoil) is a borough (Estonian: alev) in Rapla County, ...

 

 

Oliver Twist

Oliver Twist Halaman depan edisi pertama tahun 1838Ilustrasi dan desain oleh George CruikshankPengarangCharles DickensJudul asliOliver Twist; atau, The Parish Boy's ProgressIlustratorGeorge CruikshankNegaraInggrisBahasaInggrisSeriBulanan:Februari 1837 – April 1839GenreFiksiKritik sosialPenerbitSerial:Bentley's Miscellany Buku:Richard BentleyTanggal terbit1837 (dalam tiga volume)Jenis mediaCetakHalaman123 settingISBNISBN 91-1-937201-9OCLC185812519 Oliver Twist adalah judul seb...

 

 

Sitoskeleton

Biologi selSel hewanKomponen sel hewan pada umumnya: Nukleolus Inti sel Ribosom (titik-titik kecil sebagai bagian dari no. 5) Vesikel Retikulum endoplasma kasar Badan Golgi Sitoskeleton Retikulum endoplasma halus Mitokondria Vakuola Sitosol (cairan yang berisi organel, yang terdiri dari sitoplasma) Lisosom Sentrosom Membran sel Sitoskeleton eukariota. Aktin digambarkan dengan warna merah dan mikrotubulus dengan warna hijau. Struktur berwarna biru ialah inti sel. Sitoskeleton, rangka sel, atau...

Человек прямоходящий

† Человек прямоходящий Научная классификация Домен:ЭукариотыЦарство:ЖивотныеПодцарство:ЭуметазоиБез ранга:Двусторонне-симметричныеБез ранга:ВторичноротыеТип:ХордовыеПодтип:ПозвоночныеИнфратип:ЧелюстноротыеНадкласс:ЧетвероногиеКлада:АмниотыКлада:Синапсиды�...

 

 

Cittadinanza israeliana

La copertina di un passaporto biometrico israeliano contemporaneo rilasciato dal 2013. La cittadinanza israeliana (חוק האזרחות) è un sistema di relazioni politiche e legali tra una persona e lo stato di Israele, espresse nei loro diritti e doveri reciproci. Le condizioni per l'acquisizione e la cessazione della cittadinanza israeliana sono previste dalla legge israeliana, in primo luogo la Legge sulla cittadinanza, adottata il 1º aprile 1952. Cittadinanza La cittadinanza può ess...

 

 

قهوة إيرلندية

كاس من القهوة الأيرلندية القهوة الأيرلندية (بالإيرلندية Caife Gaelach) عبارة عن كوكتيل مكون من خليط القهوة الساخنة، الويسكي الإيرلندي والسكر مع الكريمة.[1][2][3] بحسب الوصفة الأصلية الكريمة غير مخفوقة مع أن البعض يستعمل الكريمة المخفوقة. ينسب اختراع هذا المشروب إلى جو�...

Национальное аэрокосмическое агентство Азербайджана

Национальное аэрокосмическое агентство Азербайджана Штаб-квартира Баку, ул. С. Ахундова, AZ 1115 Локация  Азербайджан Тип организации Космическое агентство Руководители Директор: Натиг Джавадов Первый заместитель генерального директора Тофик Сулейманов Основание Осн�...

 

 

The Gospel of the Flying Spaghetti Monster

Book by Bobby Henderson The Gospel of theFlying Spaghetti Monster First edition cover – designed to look like a hardbackAuthorBobby HendersonCountryUnited StatesLanguageEnglishGenreSatirical religious textPublisherVillard BooksPublication dateMarch 28, 2006Media typePrint (Paperback)Pages192 ppISBN0-8129-7656-8OCLC65065501Dewey Decimal818/.607 22LC ClassPN6231.R4 H46 2006 The Gospel of the Flying Spaghetti Monster is a satirical book written by Bobby Henderson that embodies t...

 

 

العلاقات الألبانية الكورية الشمالية

العلاقات الألبانية الكورية الشمالية ألبانيا كوريا الشمالية   ألبانيا   كوريا الشمالية تعديل مصدري - تعديل   العلاقات الألبانية الكورية الشمالية هي العلاقات الثنائية التي تجمع بين ألبانيا وكوريا الشمالية.[1][2][3][4][5] مقارنة بين البلدين هذه �...

نظام لبنان

تحتاج هذه المقالة إلى الاستشهاد بمصادر إضافية لتحسين وثوقيتها. فضلاً ساهم في تطوير هذه المقالة بإضافة استشهادات من مصادر موثوق بها. من الممكن التشكيك بالمعلومات غير المنسوبة إلى مصدر وإزالتها. (نوفمبر 2010) تحتوي هذه المقالة أبحاثًا أصيلةً، وهذا مُخَالفٌ لسياسات الموسوعة. ف...

 

 

2017 Orkney Islands Council election

2017 Scottish local government election 2017 Orkney Islands Council election ← 2012 4 May 2017 (2017-05-04) 2022 → All 21 seats to Orkney Islands Council11 seats needed for a majorityTurnout7,470 (49.7%)   First party Second party Third party   Leader Steven Heddle Rachael King[1] Steve Sankey Party Independent Orkney Manifesto Group Scottish Green Leader's seat Kirkwall East West Mainland East Mainland, South Ronaldsay and Burray La...

 

 

WCWF

CW affiliate in Suring, Wisconsin For the station in Saranac Lake, New York, previously known by this call sign, see WYCI. WCWFATSC 3.0 stationSuring–Green Bay, WisconsinUnited StatesCitySuring, WisconsinChannelsDigital: 15 (UHF)Virtual: 14BrandingCW14ProgrammingAffiliations14.1: The CW / Fox (alternate) / ABC (secondary)for others, see § SubchannelsOwnershipOwnerSinclair Broadcast Group(WCWF Licensee, LLC)Sister stationsWLUK-TVHistoryFoundedJuly 27, 1981[1]First air dateFebru...

City of Preston, Lancashire

City and non-metropolitan district in Lancashire, England This article is about the local government district of Lancashire. For the settlement after which it is named, see Preston, Lancashire. For other uses, see City of Preston and Preston. City & Non-metropolitan district in EnglandCity of PrestonCity & Non-metropolitan districtPrestonMiller Park in Preston Coat of Arms of the City CouncilMotto: Prince of Peace (Princeps Pacis)Shown within Lancashire and EnglandCity of Preston...

 

 

Berg-op-Zoom

Cet article est une ébauche concernant une localité néerlandaise. Vous pouvez partager vos connaissances en l’améliorant (comment ?) selon les recommandations des projets correspondants. Consultez la liste des tâches à accomplir en page de discussion. Pour les articles homonymes, voir Bergen, Berg et Zoom (homonymie). Berg-op-Zoom Armoiries. Drapeau. La grand-place de Berg-op-Zoom Noms Nom néerlandais Bergen op Zoom Administration Pays Pays-Bas Province Brabant-Septentrional Bo...

 

 

1943 in baseball

See also: 1943 Major League Baseball season and 1943 All-American Girls Professional Baseball League season The following are the baseball events of the year 1943 throughout the world. Overview of the events of 1943 in baseball Years in baseball ← 1940 1941 1942 1943 1944 1945 1946 → 1943 in sports Air sports American football Aquatic sports Association football Athletics Australian rules football Badminton Baseball Basketball Canadian football Chess Climbing Combat sports Sumo Cricket 19...

1820 en arts plastiques

Cet article est une ébauche concernant l’art et une chronologie ou une date. Vous pouvez partager vos connaissances en l’améliorant (comment ?) selon les recommandations des projets correspondants. Chronologies Données clés 1817 1818 1819  1820  1821 1822 1823Décennies :1790 1800 1810  1820  1830 1840 1850Siècles :XVIIe XVIIIe  XIXe  XXe XXIeMillénaires :-Ier Ier  IIe  IIIe Chronologies géographiques Afrique Afrique du S...

 

 

Il massaggio

Il massaggioAutoreÉdouard Debat-Ponsan Data1883 Tecnicaolio su tela Dimensioni127×210 cm UbicazioneMusée des Augustins, Tolosa Il massaggio all'hammam[1] o Il massaggio[2] (Le Massage, scène de Hammam) è un dipinto a olio su tela realizzato dall'artista francese Édouard Debat-Ponsan nel 1883 ed esposto al musée des Augustins di Tolosa.[3] Indice 1 Storia 2 Descrizione 3 Analisi 4 Note 5 Altri progetti Storia Dipinta nel 1883, di ritorno da un viaggio nella ...