In music, 41 equal temperament, abbreviated 41-TET, 41-EDO, or 41-ET, is the tempered scale derived by dividing the octave into 41 equally sized steps (equal frequency ratios). Play^ⓘ Each step represents a frequency ratio of 2^1/41, or 29.27 cents (Play^ⓘ), an interval close in size to the septimal comma. 41-ET can be seen as a tuning of the schismatic,^[1] magic and miracle^[2] temperaments. It is the second smallest equal temperament, after 29-ET, whose perfect fifth is closer to just intonation than that of 12-ET. In other words, ${\displaystyle 2^{24/41}\approx 1.50042}$ is a better approximation to the ratio ${\displaystyle 3/2=1.5}$ than either ${\displaystyle 2^{17/29}\approx 1.50129}$ or ${\displaystyle 2^{7/12}\approx 1.49831}$.

Although 41-ET has not seen as wide use as other temperaments such as 19-ET or 31-ET ^{[citation needed]}, pianist and engineer Paul von Janko built a piano using this tuning, which is on display at the Gemeentemuseum in The Hague.^[3] 41-ET can also be seen as an octave-based approximation of the Bohlen–Pierce scale.

41-ET guitars have been built, notably by Yossi Tamim. The frets on such guitars are very tightly spaced. To make a more playable 41-ET guitar, an approach called "The Kite Tuning" omits every-other fret (in other words, 41 frets per two octaves or 20.5 frets per octave) while tuning adjacent strings to an odd number of steps of 41. ^[4] Thus, any two adjacent strings together contain all the pitch classes of the full 41-ET system. The Kite Guitar's main tuning uses 13 steps of 41-ET (which approximates a 5/4 ratio) between strings. With that tuning, all simple ratios of odd limit 9 or less are available at spans at most only 4 frets.

41-ET is also a subset of 205-ET, for which the keyboard layout of the Tonal Plexus is designed.

Here are the sizes of some common intervals (shaded rows mark relatively poor matches):

As the table above shows, the 41-ET both distinguishes between and closely matches all intervals involving the ratios in the harmonic series up to and including the 10th overtone. This includes the distinction between the major tone and minor tone (thus 41-ET is not a meantone tuning). These close fits make 41-ET a good approximation for 5-, 7- and 9-limit music.

41-ET also closely matches a number of other intervals involving higher harmonics. It distinguishes between and closely matches all intervals involving up through the 12th overtones, with the exception of the greater undecimal neutral second (11:10). Although not as accurate, it can be considered a full 15-limit tuning as well.

Intervals not tempered out by 41-ET include the lesser diesis (128:125), septimal diesis (49:48), septimal sixth-tone (50:49), septimal comma (64:63), and the syntonic comma (81:80).

41-ET tempers out 100:99, which is the difference between the greater undecimal neutral second and the minor tone, as well as the septimal kleisma (225:224), 1029:1024 (the difference between three intervals of 8:7 the interval 3:2), and the small diesis (3125:3072).

Using extended pythagorean notation results in double and even triple sharps and flats. Furthermore, the notes run out of order. The chromatic scale is C, B♯, A/E, D♭, C♯, B, E, D... These issues can be avoided by using ups and downs notation.^[5] The up and down arrows are written as a caret or a lower-case "v", usually in a sans-serif font. One arrow equals one step of 41-TET. In note names, the arrows come first, to facilitate chord naming. The many enharmonic equivalences allow great freedom of spelling.

• C, ^C, ^^C/vvC♯/vD♭, vC♯/D♭, C♯/^D♭, ^C♯/^^D♭/vvD, vD,
• D, ^D, ^^D/vvD♯/vE♭, vD♯/E♭, D♯/^E♭, ^D♯/^^E♭/vvE, vE,
• E, ^E/vvF, ^^E/vF,
• F, ^F, ^^F/vvF♯/vG♭, vF♯/G♭, F♯/^G♭, ^F♯/^^G♭/vvG, vG,
• G, ^G, ^^G/vvG♯/vA♭, vG♯/A♭, G♯/^A♭, ^G♯/^^A♭/vvA, vA,
• A, ^A, ^^A/vvA♯/vB♭, vA♯/B♭, A♯/^B♭, ^A♯/^^B♭/vvB, vB,
• B, ^B/vvC, ^^B/vC, C

Because ups and downs notation names the intervals of 41-TET,^[6] it can provide precise chord names. The pythagorean minor chord with 32/27 on C is still named Cm and still spelled C–E♭–G. But the 5-limit upminor chord uses the upminor 3rd 6/5 and is spelled C–^E♭–G. This chord is named C^m. Compare with ^Cm (^C–^E♭–^G).