N-karbamoil-L-aminokiselinska hidrolaza

N-karbamoil-L-aminokiselinska hidrolaza
Identifikatori
EC broj3.5.1.87
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BRENDABRENDA pristup
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Strukture PBPRCSB PDB PDBe PDBj PDBsum

N-karbamoil-L-aminokiselinska hidrolaza (EC 3.5.1.87, N-karbamil L-aminokiselinska amidohidrolaza, N-karbamoil-L-aminokiselinska amidohidrolaza, L-N-karbamoilaza, N-karbamoilaza) je enzim sa sistematskim imenom N-karbamoil-L-aminokiselina amidohidrolaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju

N-karbamoil-L-2-aminokiselina (2-ureido karboksilat) + H2O L-2-aminokiselina + NH3 + CO2

Ovaj enzim zajedno sa EC 3.5.1.77 (N-karbamoil-D-aminokiselinskom hidrolazom), EC 5.1.99.5 (hidantoinskom racemazom) i hidantoinazom, formira deo reakcione kaskade poznate kao hidantoinazni proces.

Reference

  1. ^ Ogawa, J., Miyake, H. and Shimizu, S. (1995). „Purification and characterization of N-carbamoyl-L-amino acid amidohydrolase with broad substrate specificity from Alcaligenes xylosoxidans”. Appl. Microbiol. Biotechnol. 43: 1039—1043. PMID 8590654. 
  2. ^ Martínez-Rodríguez, S., Clemente-Jiménez, J.M., Rodríguez-Vico, F. and Las Heras-Vázquez, F.J. (2005). „Molecular cloning and biochemical characterization of L-N-carbamoylase from Sinorhizobium meliloti CECT4114”. J. Mol. Microbiol. Biotechnol. 9: 16—25. PMID 16254442. 
  3. ^ Altenbuchner, J., Siemann-Herzberg, M. and Syldatk, C. (2001). „Hydantoinases and related enzymes as biocatalysts for the synthesis of unnatural chiral amino acids”. Curr. Opin. Biotechnol. 12: 559—563. PMID 11849938. 

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