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PubMed	članci
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Fitoen desaturaza (formira neurosporen)
Fitoen desaturaza (formira neurosporen)
Identifikatori
EC broj	1.3.99.28
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Fitoen desaturaza (formira neurosporen) (EC 1.3.99.28, trostepna fitoenska desaturaza, fitoenska desaturaza (nespecifična), CrtI (nespecifična)) je enzim sa sistematskim imenom 15-cis-fitoen:akceptor oksidoreduktaza (formira neurosporen).^[1]^[2] Ovaj enzim katalizuje sledeću hemijsku reakciju

15-cis-fitoen + 3 akceptor

\rightleftharpoons

sve-trans-neurosporen + 3 redukovani akceptor (sveukupna reakcija)

(1a) 15-cis-fitoen + akceptor

\rightleftharpoons

sve-trans-fitofluen + redukovani akceptor

(1b) sve-trans-fitofluen + akceptor

\rightleftharpoons

sve-trans-zeta-karoten + redukovani akceptor

(1c) sve-trans-zeta-karoten + akceptor

\rightleftharpoons

sve-trans-neurosporen + redukovani akceptor

Ovaj enzim učestvuje u karotenoidnoj biosintezi. On katalizuje od jednog do tri koraka desaturacije, cf. EC 1.3.99.29, fitoenska desaturaza (formira zeta-karoten), EC 1.3.99.30, fitoenska desaturaza (formira 3,4-didehidrolikopen), EC 1.3.99.31, fitoenska desaturaza (formira likopen). Ovaj enzim aktivfira FAD.

Reference

^ Raisig, A., Bartley, G., Scolnik, P. and Sandmann, G. (1996). „Purification in an active state and properties of the 3-step phytoene desaturase from Rhodobacter capsulatus overexpressed in Escherichia coli”. J. Biochem. 119: 559—564. PMID 8830054.
^ Wang, C.W. & Liao, J.C. (2001). „Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution”. J. Biol. Chem. 276: 41161—41164. PMID 11526111.

Literatura

Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.

Spoljašnje veze

Phytoene+desaturase+(neurosporene-forming) на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Raisig, A., Bartley, G., Scolnik, P. and Sandmann, G. (1996). „Purification in an active state and properties of the 3-step phytoene desaturase from Rhodobacter capsulatus overexpressed in Escherichia coli”. J. Biochem. 119: 559—564. PMID 8830054.

[2] Wang, C.W. & Liao, J.C. (2001). „Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution”. J. Biol. Chem. 276: 41161—41164. PMID 11526111.

[1]

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