Asparaginska sintaza (glutamin-hidrolizujuća)

Asparaginska sintaza (glutamin-hidrolizujuća)
Asparaginska sintetaza B, dimer, E.Coli
Identifikatori
EC broj6.3.5.4
CAS broj37318-72-2
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Asparaginska sintaza (glutamin-hidrolizujuća) (EC 6.3.5.4, asparaginska sintetaza (glutaminska hidroliza), glutamin-zavisna asparaginska sintetaza, asparaginska sintetaza B, AS, AS-B) je enzim sa sistematskim imenom L-aspartat:L-glutamin amido-ligaza (formira AMP).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

ATP + L-aspartat + L-glutamin + H2O AMP + difosfat + L-asparagin + L-glutamat (sveukupna reakcija)
(1a) L-glutamin + H2O L-glutamat + NH3
(1b) ATP + L-aspartat + NH3 AMP + difosfat + L-asparagin

Enzim iz Escherichia coli ima dva aktivna mesta.

Reference

  1. ^ Patterson, M.K., Jr. & Orr, G.R. (1968). „Asparagine biosynthesis by the Novikoff hepatoma. Isolation, purification, property, and mechanism studies of the enzyme system”. J. Biol. Chem. 243: 376—380. PMID 4295091. 
  2. ^ Boehlein, S.K., Richards, N.G. and Schuster, S.M. (1994). „Glutamine-dependent nitrogen transfer in Escherichia coli asparagine synthetase B. Searching for the catalytic triad”. J. Biol. Chem. 269: 7450—7457. PMID 7907328. 
  3. ^ Richards, N.G. & Schuster, S.M. (1998). „Mechanistic issues in asparagine synthetase catalysis”. Adv. Enzymol. Relat. Areas Mol. Biol. 72: 145—198. PMID 9559053. 
  4. ^ Larsen, T.M., Boehlein, S.K., Schuster, S.M., Richards, N.G., Thoden, J.B., Holden, H.M. and Rayment, I. (1999). „Three-dimensional structure of Escherichia coli asparagine synthetase B: a short journey from substrate to product”. Biochemistry. 38: 16146—16157. PMID 10587437. 
  5. ^ Huang, X., Holden, H.M. and Raushel, F.M. (2001). „Channeling of substrates and intermediates in enzyme-catalyzed reactions”. Annu. Rev. Biochem. 70: 149—180. PMID 11395405. 
  6. ^ Tesson, A.R., Soper, T.S., Ciustea, M. and Richards, N.G. (2003). „Revisiting the steady state kinetic mechanism of glutamine-dependent asparagine synthetase from Escherichia coli”. Arch. Biochem. Biophys. 413: 23—31. PMID 12706338. 

Literatura

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