2,3-Dihidroksibenzoat-serinska ligaza

2,3-Dihidroksibenzoat-serinska ligaza
Identifikatori
EC broj6.3.2.14
CAS broj37318-63-1
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

2,3-Dihidroksibenzoat-serinska ligaza (EC 6.3.2.14, N-(2,3-dihidroksibenzoil)-serin sintetaza, 2,3-dihidroksibenzoilserin sintetaza, 2,3-dihidroksibenzoat—serin ligaza) je enzim sa sistematskim imenom 2,3-dihidroksibenzoat:L-serin ligaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

6 ATP + 3 2,3-dihidroksibenzoat + 3 L-serin enterobactin + 6 AMP + 6 difosfat

Ovaj enzimski kompleks katalizuje konverziju molekula koji se formiraju siderofori enterobaktina.

Reference

  1. ^ Brot, N. & Goodwin, J. (1968). „Regulation of 2,3-dihydroxybenzoylserine synthetase by iron”. J. Biol. Chem. 243: 510—513. PMID 4966114. 
  2. ^ Rusnak, F., Faraci, W.S. and Walsh, C.T. (1989). „Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product”. Biochemistry. 28: 6827—6835. PMID 2531000. 
  3. ^ Rusnak, F., Liu, J., Quinn, N., Berchtold, G.A. and Walsh, C.T. (1990). „Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase”. Biochemistry. 29: 1425—1435. PMID 2139796. 
  4. ^ Rusnak, F., Sakaitani, M., Drueckhammer, D., Reichert, J. and Walsh, C.T. (1991). „Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine”. Biochemistry. 30: 2916—2927. PMID 1826089. 
  5. ^ Gehring, A.M., Mori, I. and Walsh, C.T. (1998). „Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF”. Biochemistry. 37: 2648—2659. PMID 9485415. 
  6. ^ Shaw-Reid, C.A., Kelleher, N.L., Losey, H.C., Gehring, A.M., Berg, C. and Walsh, C.T. (1999). „Assembly line enzymology by multimodular nonribosomal peptide synthetases: the thioesterase domain of E. coli EntF catalyzes both elongation and cyclolactonization”. Chem. Biol. 6: 385—400. PMID 10375542. 

Literatura

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