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Tropinon reduktaza
Identifikatori
EC broj	1.1.1.236
CAS broj	136111-61-0
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

Tropinon reduktaza (EC 1.1.1.236, tropinon reduktaza (formira psi-tropin), tropinon reduktaza (formira pseudotropin), tropinon reduktaza (ambiguous), TR-II) je enzim sa sistematskim imenom pseudotropin:NADP⁺ 3-oksidoreduktaza.^[1]^[2]^[3]^[4] Ovaj enzim katalizuje sledeću hemijsku reakciju

pseudotropin + NADP⁺

\rightleftharpoons

tropinon + NADPH + H⁺

Ovaj enzim zajedno sa EC 1.1.1.206, tropinskom dehidrogenazom, predstavlja tačku granaja u metabolizmu tropanskih alkaloida. Tropin (produkt enzima EC 1.1.1.206) se inkorporira u hioscijamin i skopolamin, dok je pseudotropin (product enzima EC 1.1.1.236) prvi specifični metabolit na putu do kalistegina. Oba enzima su uvek prisutna u vrstama koje proizvode tropanske alkaloide. Oni imaju zajednički supstrat, tropinon, i strogo su stereospecifični.

Reference

↑ Dräger, B., Hashimoto, T. and Yamada, Y. (1988). „Purification and characterization of pseudotropine forming tropinone reductase from Hyoscyamus niger root cultures”. Agric. Biol. Chem. 52: 2663-2667.
↑ Couladis, M.M, Friesen, J.B., Landgrebe, M.E. and Leete, E. (1991). „Enzymes catalysing the reduction of tropinone to tropine and ψ-tropine isolated from the roots of Datura innoxia”. Pytochemistry 30: 801-805.
↑ Nakajima, K., Hashimoto, T. and Yamada, Y. (1993). „Two tropinone reductases with different stereospecificities are short-chain dehydrogenases evolved from a common ancestor”. Proc. Natl. Acad. Sci. USA 90: 9591-9595. PMID 8415746.
↑ Dräger, B. (2006). „Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism”. Phytochemistry 67: 327-337. PMID 16426652.

Literatura

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Vanjske veze

MeSH Tropinone+reductase+II

[1] Dräger, B., Hashimoto, T. and Yamada, Y. (1988). „Purification and characterization of pseudotropine forming tropinone reductase from Hyoscyamus niger root cultures”. Agric. Biol. Chem. 52: 2663-2667.

[2] Couladis, M.M, Friesen, J.B., Landgrebe, M.E. and Leete, E. (1991). „Enzymes catalysing the reduction of tropinone to tropine and ψ-tropine isolated from the roots of Datura innoxia”. Pytochemistry 30: 801-805.

[3] Nakajima, K., Hashimoto, T. and Yamada, Y. (1993). „Two tropinone reductases with different stereospecificities are short-chain dehydrogenases evolved from a common ancestor”. Proc. Natl. Acad. Sci. USA 90: 9591-9595. PMID 8415746.

[4] Dräger, B. (2006). „Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism”. Phytochemistry 67: 327-337. PMID 16426652.

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