Lakto-N-biozidaza

Lakto-N-biozidaza
Identifikatori
EC broj 3.2.1.140
CAS broj 146359-52-6
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB RCSB PDB PDBe PDBj PDBsum

Lakto-N-biozidaza (EC 3.2.1.140, Lacto-N-biosidase) je enzim sa sistematskim imenom oligosaharid lakto-N-biozilhidrolaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) beta-D-GaL-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-GaL-(1->4)-D-Glc + H2O beta-D-GaL-(1->3)-D-GlcNAc + beta-D-GaL-(1->4)-D-Glc
(2) lakto-N-tetraoza + H2O lakto-N-bioza + laktoza

Enzim iz Streptomyces specifično hidrolizuje terminalne lakto-N-biozilne ostatake (beta-D-Gal-(1->3)-D-GlcNAc) iz neredukujućeg kraja oligosaharida sa strukturom beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R).

Reference

  1. ↑ Sano, M., Hayakawa, K., Kato, I. (1992). „An enzyme releasing lacto-N-biose from oligosaccharides”. Proc. Natl. Acad. Sci. USA 89: 8512-8516. PMID 1528855. 
  2. ↑ Sano, M., Hayakawa, K., Kato, I. (1993). „Purification and characterization of an enzyme releasing lacto-N-biose from oligosaccharides with type 1 chain”. J. Biol. Chem. 268: 18560-18566. PMID 7689556. 

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