MLS Best XI on jalkapalloliiga Major League Soccerin kausittain valittava tähdistöjoukkue eli liigan 11 parasta pelaajaa. Joukkueen valitsevat pelaajat, valmentajat, urheilutoimenjohtajat ja tiedotusvälineiden edustajat.^[1]

Kausi	Maalivahti	Puolustajat	Keskikenttäpelaajat	Hyökkääjät
1996[2]	Mark Dodd (Dallas)	Leonel Álvarez (Dallas) John Doyle (San Jose) Robin Fraser (Los Angeles)	Mauricio Cienfuegos (Los Angeles) Roberto Donadoni (MetroStars) Marco Etcheverry (D.C. United) Preki (Kansas City) Carlos Valderrama (Tampa Bay)	Eduardo Hurtado (Los Angeles) Roy Lassiter (Tampa Bay)
1997[2]	Brad Friedel (Columbus)	Jeff Agoos (D.C. United) Thomas Dooley (Columbus) Richard Gough (Kansas City) Eddie Pope (D.C. United)	Mark Chung (Kansas City) Marco Etcheverry (D.C. United) Preki (Kansas City) Carlos Valderrama (Tampa Bay)	Ronald Cerritos (San Jose) Jaime Moreno (D.C. United)
1998[2]	Zach Thornton (Chicago)	Thomas Dooley (Columbus) Robin Fraser (Los Angeles) Luboš Kubík (Chicago) Eddie Pope (D.C. United)	Chris Armas (Chicago) Mauricio Cienfuegos (Los Angeles) Marco Etcheverry (D.C. United) Piotr Nowak (Chicago)	Stern John (Columbus) Cobi Jones (Los Angeles)
1999[2]	Kevin Hartman (Los Angeles)	Jeff Agoos (D.C. United) Robin Fraser (Los Angeles) Luboš Kubík (Chicago)	Chris Armas (Chicago) Mauricio Cienfuegos (Los Angeles) Marco Etcheverry (D.C. United) Eddie Lewis (San Jose) Steve Ralston (Tampa Bay)	Jason Kreis (Dallas) Jaime Moreno (D.C. United)
2000[2]	Tony Meola (Kansas City)	Robin Fraser (Los Angeles) Greg Vanney (Los Angeles) Peter Vermes (Kansas City)	Chris Armas (Chicago) Piotr Nowak (Chicago) Steve Ralston (Tampa Bay) Hristo Stoitškov (Chicago) Carlos Valderrama (Tampa Bay)	Mamadou Diallo (Tampa Bay) Clint Mathis (MetroStars)
2001[2]	Tim Howard (MetroStars)	Jeff Agoos (San Jose) Carlos Llamosa (Miami) Pablo Mastroeni (Miami) Greg Vanney (Los Angeles)	Chris Armas (Chicago) Piotr Nowak (Chicago) Preki (Miami) John Spencer (Colorado)	Alex Pineda Chacón (Miami) Diego Serna (Miami)
2002[2]	Tim Howard (MetroStars)	Wade Barrett (San Jose) Carlos Bocanegra (Chicago) Alexi Lalas (Los Angeles)	Mark Chung (Colorado) Jeff Cunningham (Columbus) Ronnie Ekelund (San Jose) Óscar Pareja (Dallas) Steve Ralston (New England)	Carlos Ruiz (Los Angeles) Taylor Twellman (New England)
2003[2]	Pat Onstad (San Jose)	Carlos Bocanegra (Chicago) Ryan Nelsen (D.C. United) Eddie Pope (MetroStars)	Chris Armas (Chicago) DaMarcus Beasley (Chicago) Mark Chung (Colorado) Landon Donovan (San Jose) Preki (Kansas City)	John Spencer (Colorado) Ante Razov (Chicago)
2004[2]	Joe Cannon (Colorado)	Jimmy Conrad (Kansas City) Robin Fraser (Columbus) Ryan Nelsen (D.C. United) Eddie Pope (MetroStars)	Eddie Gaven (MetroStars) Amado Guevara (MetroStars) Ronnie O'Brien (Dallas) Kerry Zavagnin (Kansas City)	Brian Ching (San Jose) Jaime Moreno (D.C. United)
2005[2]	Pat Onstad (San Jose)	Chris Albright (Los Angeles) Danny Califf (San Jose) Jimmy Conrad (Kansas City)	Dwayne De Rosario (San Jose) Clint Dempsey (New England) Christian Gómez (D.C. United) Shalrie Joseph (New England) Ronnie O'Brien (Dallas)	Jaime Moreno (D.C. United) Taylor Twellman (New England)
2006[2]	Troy Perkins (D.C. United)	Bobby Boswell (D.C. United) José Burciaga Jr. (Kansas City) Jimmy Conrad (Kansas City)	Ricardo Clark (Houston) Dwayne De Rosario (Houston) Clint Dempsey (New England) Christian Gómez (D.C. United) Justin Mapp (Chicago)	Jeff Cunningham (Salt Lake) Jaime Moreno (D.C. United)
2007[2]	Brad Guzan (Chivas USA)	Jonathan Bornstein (Chivas USA) Michael Parkhurst (New England) Eddie Robinson (Houston)	Guillermo Barros Schelotto (Columbus) Dwayne De Rosario (Houston) Christian Gómez (D.C. United) Shalrie Joseph (New England) Ben Olsen (D.C. United)	Juan Pablo Ángel (New York) Luciano Emílio (D.C. United)
2008[2]	Jon Busch (Chicago)	Jimmy Conrad (Kansas City) Chad Marshall (Columbus) Bakary Soumare (Chicago)	Guillermo Barros Schelotto (Columbus) Cuauhtémoc Blanco (Chicago) Shalrie Joseph (New England) Sacha Kljestan (Chivas USA) Robbie Rogers (Columbus)	Kenny Cooper (Dallas) Landon Donovan (Los Angeles)
2009[1]	Zach Thornton (Chivas USA)	Geoff Cameron (Houston) Wilman Conde (Chicago) Chad Marshall (Columbus)	Dwayne De Rosario (Toronto) Landon Donovan (Los Angeles) Stuart Holden (Houston) Shalrie Joseph (New England) Fredrik Ljungberg (Seattle)	Conor Casey (Colorado) Jeff Cunningham (Dallas)
2010[3]	Donovan Ricketts (Los Angeles)	Nat Borchers (Salt Lake) Omar Gonzalez (Los Angeles) Jámison Olave (Salt Lake)	Dwayne De Rosario (Toronto) Landon Donovan (Los Angeles) David Ferreira (Dallas) Sébastien Le Toux (Philadelphia) Javier Morales (Salt Lake)	Edson Buddle (Los Angeles) Chris Wondolowski (San Jose)

• 5: Chris Armas, Dwayne De Rosario, Robin Fraser, Jaime Moreno
• 4: Jimmy Conrad, Landon Donovan, Marco Etcheverry, Shalrie Joseph, Eddie Pope, Preki
• 3: Jeff Agoos, Mauricio Cienfuegos, Jeff Cunningham, Christian Gómez, Piotr Nowak, Steve Ralston, Carlos Valderrama

Liigan kymmenennen kauden kunniaksi vuonna 2005 valittiin myös liigan koko siihenastisen historian tähdistöjoukkue, MLS All-Time Best XI. Ehdolla olivat aikaisemmin MLS Best XI -joukkueeseen valitut pelaajat ja vähintään kolmesti MLS All-Star Game -otteluun valitut pelaajat, yhteensä 78 pelaajaa. Joukkue valittiin liigan verkkosivustolla olleen avoimen äänestyksen (50 prosentin painoarvo) sekä tiedotusvälineiden edustajien (25 prosenttia) ja MLS-joukkueiden valmentajien ja urheilutoimenjohtajien (25 prosenttia) äänten perusteella.^[4]

Maalivahti	Puolustajat	Keskikenttäpelaajat	Hyökkääjät
Tony Meola (MetroStars, Kansas City)	Jeff Agoos (D.C. United, San Jose, MetroStars) Marcelo Balboa (Colorado, MetroStars) Eddie Pope (D.C. United, MetroStars, Salt Lake)	Landon Donovan (San Jose, Los Angeles) Marco Etcheverry (D.C. United) Piotr Nowak (Chicago) Preki (Kansas City, Miami) Carlos Valderrama (Tampa Bay, Miami, Colorado)	Brian McBride (Columbus) Jaime Moreno (D.C. United, MetroStars)

• MLS Most Valuable Player Award