Barnes started took up refereeing aged 15 with Gloucester & District Referees. At university he transferred to the Norfolk Referees Society, part of Eastern Counties (ECRURF) followed by a transfer to London Society of RFU Referees.[4] In 2001, at the age of 21, Barnes became the youngest referee ever appointed to the Panel of National Referees.[5] He became a professional referee in April 2005.[4]
He was appointed in 2008 to take charge of his first Heineken Cup knockout match, between Stade Toulousain and Cardiff Blues at Le Stadium on 6 April 2008. In 2010, Barnes officiated his first Heineken Cup Final between Toulouse and Biarritz at the Stade de France, Saint-Denis, on 22 May.
After officiating at his second Rugby World Cup in New Zealand in 2011 and refereeing the third/fourth place game between Wales and Australia, Barnes refereed a Heineken Cup semi-final on Sunday 29 April 2012; Clermont Auvergne v Leinster.[10]
Barnes was one of the officials at the Pacific Nations' Cup in Japan in 2013.[11]
On 25 May 2013, Barnes refereed the English Premiership final between Leicester Tigers and Northampton Saints, his fifth English Premiership final, during which he sent off Northampton captain Dylan Hartley for calling Barnes "a fucking cheat”.[12] This was the first time a player had been sent off in a Premiership final.[13]
On 22 December 2017, Barnes broke the all time Premiership appearances record (191) for a referee while officiating Worcester Warriors 23–8 victory over London Irish.[15]
In January 2022, Barnes refereed his 250th Premiership Rugby match.[17] On 5 November 2022, Barnes refereed the match between Wales and New Zealand in the 2022 Autumn Nations Series. It was his 100th international match as a referee, equalling the record set by Wales' Nigel Owens.[18]
On 2 November 2023, Barnes announced his retirement from refereeing after officiating in 111 test matches, including five Rugby World Cups and 26 Six Nations matches.[20]
^"Wayne Barnes". Squire Patton Boggs. Retrieved 16 September 2023.
^"Wayne Barnes". Squire Patton Boggs. Retrieved 16 September 2023.
^ ab"Wayne Barnes Joins Elite Referees". RFU.com. 6 April 2005. Archived from the original on 29 November 2014. Retrieved 29 April 2007.{{cite web}}: CS1 maint: bot: original URL status unknown (link)