Following law school graduation, Hopkins was in private practice in Birmingham, Alabama, from 1977 to 1978. She was in private practice in Washington, D.C. from 1978 to 1991. She was in private practice in Anniston, Alabama from 1991 to 2004.
Federal judicial service
On the recommendation of Senators Richard Shelby and Jeff Sessions, Hopkins was nominated to the United States District Court for the Northern District of Alabama by President George W. Bush on October 14, 2003, to a seat vacated due to the death of Edwin L. Nelson. Hopkins was confirmed by the Senate on June 15, 2004, and received her commission on June 17, 2004. She assumed senior status on June 22, 2018. She took inactive senior status on September 1, 2019.