SB buffer

SB buffer is a buffer solution used in agarose and polyacrylamide gel electrophoresis for the separation of nucleic acids such as DNA and RNA. "SB" is a commercial trademark of Faster Better Media LLC for their sodium boric acid-based conductive medium (US Patent # 7811437), which is based on the publications of Brody and Kern.

It is made up of sodium borate, usually 1–10 mM at pH 8.0. It has a lower conductivity, produces sharper bands, and can be run at higher speeds than can gels made from TBE buffer or TAE buffer (5–35 V/cm as compared to 5–10 V/cm). At a given voltage, heat will be generated and thus the gel will be heated. However, SB buffer has lower conductivity than TBE and TAE, and thus the gel temperature is much lower than with TBE or TAE buffers. Therefore, the voltage can be increased to speed up electrophoresis so that a gel run takes only a fraction of the usual time. Downstream applications, such as isolation of DNA from a gel slice or southern blot analysis, work as expected with sodium borate gels.

LB buffer containing lithium borate is similar to sodium borate and has all of its advantages, but permits use of even higher voltages due to the lower conductivity of lithium ions as compared to sodium ions.[1] However, lithium borate is somewhat more expensive.

See also

References

  1. ^ Brody JR, Kern SE (2004). "History and principles of conductive media for standard DNA electrophoresis". Anal. Biochem. 333 (1): 1–13. doi:10.1016/j.ab.2004.05.054. PMID 15351274.


Stub icon

This biochemistry article is a stub. You can help Wikipedia by expanding it.

Read other articles:

Vehicular combat game

Video game genre Part of a series onSimulation video games Subgenres Construction and management simulation Business simulation game City-building game Government simulation Life simulation game Digital pet God game Social simulation game Dating sim Eroge Bishōjo Otome Farm life sim Immersive sim Sports game Racing game Sim racing Kart racing Sports management game Fishing video game Vehicle simulations Flight simulator Amateur flight simulation Combat flight simulator Space flight simulator...

 

 

Bunuh diri Tyler Clementi

Artikel ini sebatang kara, artinya tidak ada artikel lain yang memiliki pranala balik ke halaman ini.Bantulah menambah pranala ke artikel ini dari artikel yang berhubungan atau coba peralatan pencari pranala.Tag ini diberikan pada April 2012. Tyler ClementiLahirRidgewood, New Jersey, Amerika SerikatMeninggal22 September 2010 (umur 18)Kota New York, New York, Amerika SerikatPekerjaanMahasiswa Tyler Clementi (19 Desember 1991 – 22 September 2010) adalah mahasiswa berumur delapan...

 

 

South Carolina House of Representatives

Lower house of the General Assembly of the U.S. state of South Carolina South CarolinaHouse of RepresentativesSouth Carolina General AssemblySeal of the South Carolina House of RepresentativesTypeTypeLower House Term limitsNoneHistoryNew session startedJanuary 12, 2021LeadershipSpeakerMurrell Smith (R) since May 12, 2022 Speaker pro temporeTommy Pope (R) since December 2, 2014 Majority LeaderDavey Hiott (R) since May 12, 2022 Minority LeaderJ. Todd Rutherford (D) since January 8, 2013 Structu...

Vannes Olympique Club

Questa voce sull'argomento società calcistiche francesi è solo un abbozzo. Contribuisci a migliorarla secondo le convenzioni di Wikipedia. Vannes Olympique ClubCalcio Segni distintivi Uniformi di gara Casa Trasferta Colori sociali Bianco, nero Simboli Ermellino Dati societari Città Vannes Nazione  Francia Confederazione UEFA Federazione FFF Campionato National 2 Fondazione 1892 Rifondazione1998 Presidente Michel Jestin Allenatore Pierre Talmont Stadio Stade de la Rabin(6500 post...

 

 

Yorvit Torrealba

Venezuelan baseball player (born 1978) Baseball player Yorvit TorrealbaTorrealba with the Colorado RockiesCatcherBorn: (1978-07-19) July 19, 1978 (age 45)Caracas, VenezuelaBatted: RightThrew: RightMLB debutSeptember 5, 2001, for the San Francisco GiantsLast MLB appearanceSeptember 25, 2013, for the Colorado RockiesMLB statisticsBatting average.256Home runs56Runs batted in339 Teams San Francisco Giants (2001–2005) Seattle Mariners (2005) Colorado Rockies (...

 

 

Fairytale (Alexander Rybak song)

2009 song by Alexander Rybak FairytaleSingle by Alexander Rybakfrom the album Fairytales Released12 January 2009Recorded2008Genre Pop folk Length3:03LabelEMISongwriter(s)Alexander RybakProducer(s)Kim BergsethAlexander Rybak singles chronology Fairytale (2009) Funny Little World (2009) Eurovision Song Contest 2009 entryCountryNorwayArtist(s)Alexander RybakLanguageEnglishComposer(s)Li JianLyricist(s)Alexander RybakFinals performanceSemi-final result1stSemi-final points201Final result1stFinal po...

Nicolò Tron

Disambiguazione – Se stai cercando Nicolò Tron (1685-1771), ambasciatore, politico e imprenditore veneto, vedi Nicolò Tron (politico e imprenditore). Questa voce o sezione sull'argomento sovrani italiani non cita le fonti necessarie o quelle presenti sono insufficienti. Puoi migliorare questa voce aggiungendo citazioni da fonti attendibili secondo le linee guida sull'uso delle fonti. Nicolò TronDoge di VeneziaStemma In carica1471 –1473 PredecessoreCristoforo Moro EredeFilipp...

 

 

Nicolas Walter

Nicolas WalterBornNicolas Hardy Walter(1934-11-22)22 November 1934South London, EnglandDied7 March 2000(2000-03-07) (aged 65)Milton Keynes, EnglandEducation Rendcomb College Exeter College, Oxford Occupations Writer Journalist Movement Anarchism Anti-war Humanism Spouses Ruth Oppenheim ​ ​(m. 1962; div. 1982)​ Christine Morris ​(m. 1987)​ Children2, including Natasha WalterParentWilliam Grey Walter (father)Rela...

 

 

Pete Buttigieg

American politician (born 1982) Mayor Pete redirects here. For the documentary film, see Mayor Pete (film). Pete ButtigiegOfficial portrait, 202219th United States Secretary of TransportationIncumbentAssumed office February 3, 2021PresidentJoe BidenDeputyPolly TrottenbergPreceded byElaine Chao32nd Mayor of South BendIn officeJanuary 1, 2012 – January 1, 2020Preceded bySteve LueckeSucceeded byJames Mueller Personal detailsBornPeter Paul Montgomery Buttigieg (1982-01-19) January ...

Ahu Vinapu

Cet article est une ébauche concernant l’île de Pâques. Vous pouvez partager vos connaissances en l’améliorant (comment ?) selon les recommandations des projets correspondants. Ahu Vinapu Vue générale de l'édifice Présentation Type Ahu Caractéristiques Matériaux Pierres Géographie Coordonnées 27° 10′ 35″ sud, 109° 24′ 23″ ouest Pays Chili Géolocalisation sur la carte : île de Pâques Ahu Vinapu modifier  Ahu Vinapu est ...

 

 

Beautiful Love (film)

1951 film Beautiful LoveDirected byFrançois CampauxWritten byFrançois CampauxProduced byFrançois CampauxStarringGiselle PascalAntónio VilarOdile VersoisCinematographyRené GaveauEdited byMichelle DavidMusic byAlfred Desenclos André TheurerProductioncompanyProdexDistributed byCocinorRelease date 1 June 1951 (1951-06-01) Running time97 minutesCountryFranceLanguageFrench Beautiful Love (French: Bel amour) is a 1951 French drama film directed by François Campaux and starring ...

 

 

Txakoli

TxakoliJenisSpanish wine, dry wine dan white wine AsalSpanyol dan Chili Diperkenalkan1989 [sunting di Wikidata]lbs Tiga daerah penghasil txakoli di Basque Country.   Txakoli Álava   Txakoli Vizcaya   Txakoli Getaria Sulur txakoli di wilayah Getaria Menyajikan txakoli di San Sebastian Txakoli (diucapkan pengucapan bahasa Basque: [tʃakoˈli]) atau Chacolí (diucapkan pengucapan bahasa Spanyol: [tʃakoˈli]) adalah sebuah jenis anggur dengan keasaman...

Новотитаровское сельское поселение

Сельское поселение России (МО 2-го уровня)Новотитаровское сельское поселение Флаг[d] Герб 45°14′09″ с. ш. 38°58′16″ в. д.HGЯO Страна  Россия Субъект РФ Краснодарский край Район Динской Включает 4 населённых пункта Адм. центр Новотитаровская Глава сельского пос�...

 

 

نصب الجندي المجهول (الكرامة)

  لمعانٍ أخرى، طالع نصب الجندي المجهول (توضيح). نصب الجندي المجهول (الكرامة)معلومات عامةنوع المبنى تمثال المنطقة الإدارية محافظة البلقاء البلد  الأردن معلومات أخرىالإحداثيات 31°54′12″N 35°37′49″E / 31.90339°N 35.63033°E / 31.90339; 35.63033 تعديل - تعديل مصدري - تعديل ويكي بي...

 

 

On My Mind (Diplo and Sidepiece song)

2019 single by Diplo and SidepieceOn My MindSingle by Diplo and Sidepiecefrom the album Diplo ReleasedDecember 13, 2019 (2019-12-13)Genre House[1] disco[2] Length3:09LabelHigher GroundSongwriter(s)Missy ElliottChad James ElliottDylan RaglandGeorge PearsonMelissa ElliotRichard Cook Mears IVStingThomas Wesley PentzProducer(s)DiploSidepieceDiplo singles chronology Samba Sujo (2019) On My Mind (2019) Bubble Up (2019) Music videoOn My Mind on YouTube On My Mind i...

Memoria visual de una nación

Memoria visual de una nación Memoria visual de una nación, estación Universidad de Chile.Autor Mario ToralCreación 1993 - 1999[1]​Ubicación Estación Universidad de Chile Santiago, Chile ChileEstilo MuralismoTécnica Acrílico y óleo sobre telaDimensiones 1.200 m²[1]​Coordenadas 33°26′38″S 70°39′01″O / -33.44375, -70.65034444[editar datos en Wikidata] Memoria visual de una nación es un mural realizado por el pintor chileno Mario Tor...

 

 

Национальное аэрокосмическое агентство Азербайджана

Национальное аэрокосмическое агентство Азербайджана Штаб-квартира Баку, ул. С. Ахундова, AZ 1115 Локация  Азербайджан Тип организации Космическое агентство Руководители Директор: Натиг Джавадов Первый заместитель генерального директора Тофик Сулейманов Основание Осн�...

 

 

Museo Norton Simon

Exterior del museo Vestíbulo del museo, presidido por una estatua asiática; al fondo, una de las salas. El Museo Norton Simon (en inglés, Norton Simon Museum) es uno de los museos de patrocinio privado más destacados de los Estados Unidos. Se halla en la localidad de Pasadena, en el estado de California, y debe su nombre a su más importante benefactor: el magnate Norton Simon (Portland, 1907 - Beverly Hills, 1993), quien fue propietario de diversas empresas multinacionales, como Max Fact...

MP3

Dieser Artikel erläutert das Audio-Dateiformat. Zu anderen Bedeutungen siehe MP3 (Begriffsklärung). MPEG Audio Layer III Dateiendung: .mp3 MIME-Type: audio/mpeg  audio/MPA  audio/mpa-robust[1] Magische Zahl: FFFB hex \xFF\xFB (ASCII-C-Notation) Erstveröffentlichung: 1991 Art: Audio Standard(s): ISO/IEC 11172-3, ISO/IEC 13818-3 MP3, Eigenschreibweise mp3 (Bezeichnung nach der Dateinamenserweiterung;[2] eigentlich MPEG-1 Audio Layer III oder MPEG-2 Audio Layer III)...

 

 

AM-087

Chemical compound AM-087Identifiers IUPAC name (6aR,10aR)-3-(2-Methyl-6-bromohex-2-yl)-6,6,9-trimethyl-6a,7,10,10a-tetrahydrobenzo[c]chromen-1-ol CAS Number152674-96-9 YPubChem CID10717065ChemSpider8892405 YUNIIB4C266FTR4ChEMBLChEMBL199561 YCompTox Dashboard (EPA)DTXSID301027466 Chemical and physical dataFormulaC23H33BrO2Molar mass421.419 g·mol−13D model (JSmol)Interactive image SMILES CC3(C)C1CC=C(C)CC1c2c(O)cc(C(C)(C)CCCCBr)cc2O3 InChI InChI=1S/C23H33BrO2/c1-15-8-9-18...