Trowbridge was born in Horseheads, New York to Stephen Van Rensselaer Trowbridge (1794–1859) and Elizabeth Conkling (1797–1873) In the first year of his life, he moved with his parents and siblings in 1821 to Oakland County, Michigan, where his family settled a farm in present-day Troy.[1] He had eleven siblings, including mechanical engineer and general William Petit Trowbridge (1828–1892), and fellow general Luther Stephen Trowbridge (1836–1912).
Returning to farming after graduation, he settled in Thorndale, Michigan, in 1848 and began a political career as town supervisor.[2] In 1851 he returned to his parents' county as a farmer and became a member of the Michigan Senate, serving from 1856 to 1860 from Bloomfield, Michigan. He married Mary Ann Satterlee in 1851, with whom he had four children: Susan Elisabeth (1852), Stephen Van Rensselaer (1855), Tillman Conklin (1857), and Samuel Satterlee (1860).[2] They moved to Mary Ann's hometown of Birmingham, Michigan, in 1860, trading the Bloomfield farm for a mill there.[2]
Trowbridge's political career briefly resumed in the last few years of his life, when his college friend Rutherford Hayes was elected President of the United States. He served as commissioner of Indian Affairs under Hayes in 1880 and 1881.[2]