The enzyme rhamnulose-1-phosphate aldolase (EC 4.1.2.19) catalyzes the chemical reaction

L-rhamnulose 1-phosphate ${\displaystyle \rightleftharpoons }$ glycerone phosphate^[1] + (S)-lactaldehyde

This enzyme belongs to the family of lyases, specifically the aldehyde-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is L-rhamnulose-1-phosphate (S)-lactaldehyde-lyase (glycerone-phosphate-forming). Other names in common use include rhamnulose phosphate aldolase, L-rhamnulose 1-phosphate aldolase, L-rhamnulose-phosphate aldolase, and L-rhamnulose-1-phosphate lactaldehyde-lyase. This enzyme participates in pentose and glucuronate interconversions and fructose and mannose metabolism.

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1GT7 and 1OJR.

• Chiu TH, Feingold DS (1969). "L-rhamnulose 1-phosphate aldolase from Escherichia coli Crystallization and properties". Biochemistry. 8 (1): 98–108. doi:10.1021/bi00829a015. PMID 4975916.
• Sawada H; Takagi Y (1964). "The metabolism of L-rhamnose in Escherichia coli. 3 L-Rhamulose-phosphate aldolase". Biochim. Biophys. Acta. 92: 26–32. doi:10.1016/0926-6569(64)90265-2. PMID 14243785.