In enzymology, a retinol O-fatty-acyltransferase (EC 2.3.1.76) is an enzyme that catalyzes the chemical reaction

acyl-CoA + retinol ${\displaystyle \rightleftharpoons }$ CoA + retinyl ester

Thus, the two substrates of this enzyme are acyl-CoA and retinol, whereas its two products are CoA and retinyl ester.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:retinol O-acyltransferase. Other names in common use include retinol acyltransferase, and retinol fatty-acyltransferase. This enzyme participates in retinol metabolism.

• Helgerud P, Petersen LB, Norum KR (1983). "Retinol esterification by microsomes from the mucosa of human small intestine. Evidence for acyl-Coenzyme A retinol acyltransferase activity". J. Clin. Invest. 71 (3): 747–53. doi:10.1172/JCI110822. PMC 436925. PMID 6826734.
• Ross AC (1982). "Retinol esterification by rat liver microsomes. Evidence for a fatty acyl coenzyme A: retinol acyltransferase". J. Biol. Chem. 257 (5): 2453–9. PMID 7061433.