In enzymology, a phytanate—CoA ligase (EC 6.2.1.24) is an enzyme that catalyzes the chemical reaction

ATP + phytanate + CoA ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + phytanoyl-CoA

The 3 substrates of this enzyme are ATP, phytanate, and CoA, whereas its 3 products are AMP, diphosphate, and phytanoyl-CoA.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is phytanate:CoA ligase (AMP-forming). This enzyme is also called phytanoyl-CoA ligase.

• Muralidharan FN, Muralidharan VB (1986). "Phytanoyl-CoA ligase activity in rat liver". Biochem. Int. 13 (1): 123–30. PMID 3753503.