In enzymology, a N-hydroxy-2-acetamidofluorene reductase (EC 1.7.1.12) is an enzyme that catalyzes the chemical reaction

2-acetamidofluorene + NAD(P)+ + H₂O ${\displaystyle \rightleftharpoons }$ N-hydroxy-2-acetamidofluorene + NAD(P)H + H⁺

The 4 substrates of this enzyme are 2-acetamidofluorene, NAD⁺, NADP⁺, and H₂O, whereas its 4 products are N-hydroxy-2-acetamidofluorene, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-acetamidofluorene:NAD(P)+ oxidoreductase. Other names in common use include N-hydroxy-2-acetylaminofluorene reductase, and NAD(P)H:N-hydroxy-2-acetamidofluorene N-oxidoreductase.

• Gutmann HR, Erickson RR (1969). "The conversion of the carcinogen N-hydroxy-2-fluorenylacetamide to o-amidophenols by rat liver in vitro. An inducible enzymatic reaction". J. Biol. Chem. 244 (7): 1729–40. PMID 5780838.
• Kitamura S, Tatsumi K (1985). "Purification of N-hydroxy-2-acetylaminofluorene reductase from rabbit liver cytosol". Biochem. Biophys. Res. Commun. 133 (1): 67–74. doi:10.1016/0006-291X(85)91842-X. PMID 4074379.