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Mikhail Tsvet
Mikhail Tsvet
	Mikhail Tsvet
Born	14 May 1872; Asti, Italy
Died	26 June 1919 (aged 47); Voronezh, Russian SFSR
Nationality	Russian
Known for	Adsorption chromatography
	Scientific career
Fields	Botany

Mikhail Semyonovich Tsvet, also spelt Tsvett, Tswett, Tswet, Zwet, and Cvet (Russian: Михаил Семёнович Цвет; 14 May 1872 – 26 June 1919) was a Russian-Italian botanist who invented chromatography. His last name is Russian for "colour" and is also the root word of "flower."

Biography

Mikhail Tsvet was born on 14 May 1872 in Asti, Italy. His mother was Italian, and his father was a Russian official. His mother died soon after his birth, and he was raised in Geneva, Switzerland. He received his BS degree from the Department of Physics and Mathematics at the University of Geneva in 1893. However, he decided to dedicate himself to botany and received his PhD degree in 1896 for his work on cell physiology. He moved to Saint Petersburg, Russia, in 1896 because his father was recalled from the foreign service. There he started to work at the Biological Laboratory of the Russian Academy of Sciences. His Geneva degrees were not recognized in Russia, and he had to earn Russian degrees. In 1897 he became a teacher of botany courses for women. In 1902 he became a laboratory assistant at the Institute of Plant Physiology of the Warsaw University (now in Poland). In 1903 he became an assistant professor and taught also at other Warsaw universities. After the beginning of World War I, the Warsaw University of Technology was evacuated to Moscow, Russia, and in 1916 again to Gorki near Moscow. In 1917 he became a Professor of Botany and the director of the botanical gardens at the University of Tartu (Yuryev) (now in Estonia). In 1918 when German troops occupied the city, the university was evacuated to Voronezh, a large city in the south of Central Russia. Tsvet died of a chronic inflammation of the throat on 26 June 1919 at the age of 47.

Chromatography

Mikhail Tsvet invented chromatography in 1900 during his research on plant pigments. He used liquid-adsorption column chromatography with calcium carbonate as adsorbent and petrol ether/ethanol mixtures as eluent to separate chlorophylls and carotenoids. The method was described on 30 December 1901 at the XI Congress of Naturalists and Physicians (XI съезд естествоиспытателей и врачей) in St. Petersburg. The first printed description was in 1905, in the Proceedings of the Warsaw Society of Naturalists, biology section.^[1] He first used the term "chromatography" in print in 1906 in his two papers about chlorophyll in the German botanical journal, Berichte der Deutschen botanischen Gesellschaft.^[2]^[3] In 1907 he demonstrated his chromatograph for the German Botanical Society.

For several reasons, Tsvet's work was long ignored:^[4] the violent political upheaval in Russia at the beginning of the 20th century, the fact that Tsvet originally published only in Russian (making his results largely inaccessible to western scientists), and an article denying Tsvet's findings. Richard Willstätter and Arthur Stoll tried to repeat Tsvet's experiments, but because they used an overly aggressive adsorbent (destroying the chlorophyll), were not able to do so.^[5] They published their results and Tsvet's chromatography method fell into obscurity. It was revived 10 years after his death thanks to Austrian biochemist Richard Kuhn and his student, German scientist Edgar Lederer^[6]^[7]^[8]^[9] as well as the work of A. J. Martin and R. L. Synge.^[10]

Botanical author abbreviation

The standard botanical author abbreviation Tswett is applied to plants that he described.

References

^ Tswett, M. S. (1905) "О новой категории адсорбционных явлений и о применении их к биохимическому анализу" (O novoy kategorii adsorbtsionnykh yavleny i o primenenii ikh k biokkhimicheskomu analizu (On a new category of adsorption phenomena and on its application to biochemical analysis)), Труды Варшавского общества естествоиспытателей, отделении биологии (Trudy Varshavskago Obshchestva Estestvoispytatelei, Otdelenie Biologii (Proceedings of the Warsaw Society of Naturalists [i.e., natural scientists], Biology Section)), vol. 14, no. 6, pp. 20–39. (Note: Tsvet submitted his manuscript in 1903; however, it was not published until 1905.)
^ "Berichte der Deutschen Botanischen Gesellschaft v.24 1906". HathiTrust. Retrieved 17 October 2024.
^ Tswett, M. (1906). "Adsorptionsanalyse und chromatographische Methode. Anwendung auf die Chemie des Chlorophylls" [Adsorption analysis and chromatographic method. Application to the chemistry of chlorophyll]. Berichte der Deutschen Botanischen Gesellschaft. 24: 384–393. doi:10.1111/j.1438-8677.1906.tb06534.x. S2CID 250472633.
^ Livengood, Jonathan (2009). "Why was M. S. Tswett's chromatographic adsorption analysis rejected?". Studies in History and Philosophy of Science Part A. 40 (1): 57–69. Bibcode:2009SHPSA..40...57L. doi:10.1016/j.shpsa.2008.12.003.
^ "Justus Liebig's Annalen der Chemie v.387-389 1912". HathiTrust. Retrieved 17 October 2024.
^ Kuhn, R. and Lederer, E. (17 February 1931). "Fraktionierung und Isomerisierung des Carotins" [Fractionation and isomerization of carotene]. Die Naturwissenschaften. 19 (14): 306. Bibcode:1931NW.....19Q.306K. doi:10.1007/BF01520417. S2CID 39309723.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^ Kuhn, Richard; Winterstein, Alfred; Lederer, Edgar (1931). "Zur Kenntnis der Xanthophylle" [On our knowledge of xanthophyll]. Zeitschrift für Physiologische Chemie. 197 (1–4): 141–160. doi:10.1515/bchm2.1931.197.1-4.141.
^ Kuhn, R. and Lederer, E. (10 June 1931). "Zerlegung des Carotins in seine Komponenten. (Über das Vitamin des Wachstums, I. Mitteil)" [Separation of carotene into its components. (On the growth vitamin, 1st communication)]. Berichte der Deutschen Chemischen Gesellschaft. 64 (6): 1349–1357. doi:10.1002/cber.19310640624.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^ Kuhn, Richard and Lederer, Edgar (1931). "Über α- und β-carotin" [On α- and β-carotene]. Zeitschrift für Physiologische Chemie. 200 (4–6): 246–254. doi:10.1515/bchm2.1931.200.4-6.246.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^ Martin, A. J. and Synge, R.L. (1941). "A new form of chromatography employing two liquid phases". Biochemical Journal. 35 (12): 1358–1368. doi:10.1042/bj0351358. PMC 1265645. PMID 16747422.{{cite journal}}: CS1 maint: multiple names: authors list (link)

External links

Ostrowski, W (1968). "Michael S. Tswett—inventor of column chromatography (On the occasion of 65th anniversary of his lecture on the column chromatography technique)". Folia Biol. (Krakow). Vol. 16, no. 4. pp. 429–48. PMID 4885242.
R. Willstätter, A. Stoll, Untersuchungen über Chlorophyll, Springer, Berlin (1913)
Biography of Mikhail S. Tsvet (pdf, in German)
Mikhail S. Tsvet: Physical chemical studies on chlorophyll adsorptions Berichte der Deutschen botanischen Gesellschaft 24, 316–323 (1906)