In enzymology, a maleylacetate reductase (EC 1.3.1.32) is an enzyme that catalyzes the chemical reaction

3-oxoadipate + NAD(P)+ ${\displaystyle \rightleftharpoons }$ 2-maleylacetate + NAD(P)H + H⁺

The 3 substrates of this enzyme are 3-oxoadipate, NAD⁺, and NADP⁺, whereas its 4 products are 2-maleylacetate, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3-oxoadipate:NAD(P)+ oxidoreductase. This enzyme is also called maleolylacetate reductase. This enzyme participates in 3 metabolic pathways: gamma-hexachlorocyclohexane degradation, benzoate degradation via hydroxylation, and 1,4-dichlorobenzene degradation.

• Gaal AB, Neujahr HY (1980). "Maleylacetate reductase from Trichosporon cutaneum". Biochem. J. 185 (3): 783–6. PMC 1161460. PMID 7387635.
• Gaal AB, Neujahr HY (1981). "Induction of phenol-metabolizing enzymes in Trichosporon cutaneum". Arch. Microbiol. 130 (1): 54–58. doi:10.1007/BF00527072. PMID 7305599.