In enzymology, a malate—CoA ligase (EC 6.2.1.9) is an enzyme that catalyzes the chemical reaction

ATP + malate + CoA ${\displaystyle \rightleftharpoons }$ ADP + phosphate + malyl-CoA

The 3 substrates of this enzyme are ATP, malate, and CoA, whereas its 3 products are ADP, phosphate, and malyl-CoA.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is malate:CoA ligase (ADP-forming). Other names in common use include malyl-CoA synthetase, malyl coenzyme A synthetase, and malate thiokinase. This enzyme participates in glyoxylate and dicarboxylate metabolism.

• Mue S, Tuboi S, Kikuchi G (December 1964). "On malyl-coenzyme A synthetase". J. Biochem. 56: 545–551. PMID 14244056.