Following is a list of Fly Club members. Fly Club is a final club for male students at Harvard University. Member Initiated into the D.U. Club, which merged with the Fly Club in 1996, is indicated with a *.

• William Gardner Choate – founder of boarding school Choate Rosemary Hall^[1]
• James Bryant Conant* – 26th President of Harvard University^[2]
• Archibald Cary Coolidge – historian, Harvard professor, first director of the Harvard University Library^[1]
• Charles William Eliot – 24th President of Harvard University^[1]
• Samuel Eliot – historian; president of Trinity College, overseer of Harvard University, Boston Public Schools superintendent^[1]
• Abbott Lawrence Lowell – historian, 25th President of Harvard University^[3]
• Charles Stearns Wheeler – transcendentalist, noted as inspiration for Henry David Thoreau’s Walden^[1][4]

• Herbert Dudley Hale – Boston and New York City architect who designed the Fly Club's house at Two Holyoke Place.^[5][6][7]
• William Robert Ware – architect, first professor of architecture at MIT, founder of the School of Architecture at Columbia University^[1][8]

• Charles Francis Adams Jr. – president of the Union Pacific Railroad, president of the American Historical Association, and colonel in the Union Army^[1]
• Charlie Cheever – co-founder of Quora
• Albert Hamilton Gordon* – Wall Street entrepreneur, Chairman of Kidder Peabody
• George H. Mifflin – president of Houghton Mifflin publishing company
• Louis Kane – owner of Au Bon Pain bakery and café^[9][10]
• Spencer Rascoff – co-founder and former CEO of Zillow
• David Rockefeller* – American banker ^[11]

• Robert Carlock – screenwriter and producer^[12]
• Fred Gwynne – stage, film, and television actor
• Whit Stillman – writer-director and actor known for Metropolitan, which was nominated for the Academy Award for Best Original Screenplay
• Dustin Thomason — writer-producer known for “The Rule of Four”, “Castle Rock”, “Presumed Innocent”

• James Barr Ames – dean of Harvard Law School (1895–1910), known for popularizing the case-study method of teaching law^[1]
• James C. Carter – co-founder of law firm Carter Ledyard & Milburn^[1]
• Oliver Wendell Holmes Jr. – Supreme Court Justice^[1][5]
• John Codman Ropes – co-founder of law firm Ropes & Grey^[1][5][8]

• Robert Charles Benchley* – humorist
• James Russell Lowell – poet, critic, editor, and US ambassador to the Kingdom of Spain and the Court of St. James's^[5]
• Ernest Thayer – poet, author of "Casey at the Bat"^[13]
• Evan Thomas – journalist and author^[14]
• Owen Wister – writer, "father" of western fiction^[1][5]

• Henry L. Eustis – General in the Union Army during Civil War; dean of Lawrence Scientific School (now the Harvard John A. Paulson School of Engineering and Applied Sciences)^[1][5]
• Lionel de Jersey Harvard* – first [collateral] descendant of John Harvard to attend Harvard College, a casualty of World War I. Harvard College's Harvard-Cambridge Fellowship (to Emmanuel College) is named in his honor.^[15]

• Charles Francis Adams III – Secretary of the Navy, 1929–1932; skipper of America's Cup defender Resolute, 1920; inductee, America's Cup Hall of Fame^[1]
• Edward Bell – U.S. diplomatic official involved in the decoding of the Zimmerman Telegram in World War I^[5][16][17]
• Joseph Hodges Choate – lawyer and diplomat; U.S. Ambassador to the United Kingdom, 1899–1905^[1][5][8]
• Dwight F. Davis – U.S. Secretary of War, 1925–1929; Governor General of the Philippines, 1929–1932; tennis champion^[1][5][8]
• Grenville T. Emmet – U.S. Ambassador to Netherlands 1934–1937 and Austria 1937–1937^[1][5]
• Charles Fairchild – United States Secretary of the Treasury 1887–1889; Attorney General of New York 1876-1877^[1][5][8]
• Joseph Clark Grew – career diplomat, U.S. Ambassador to Japan 1932–1941, oversaw the development of U.S. Foreign Service^[18][19]
• Wickham Hoffman – U.S. Minister to Denmark 1883–1885; Colonel in the Union Army^[1][5]
• Jared Kushner – son-in-law of Donald Trump; Senior White House Adviser^[20] and head of the White House Office of American Innovation^[21][22]
• Tony Lake – President Bill Clinton's National Security Advisor^[2]
• James Russell Lowell – U.S. Ambassador to the Kingdom of Spain and the Court of St. James's, poet, critic, and editor^[5]
• Deval Patrick – 71st Governor of Massachusetts; quit the club in 1983^[23]
• Roger Putnam – Mayor of Springfield, Massachusetts and director of the U.S. Economic Stabilization Administration
• Jay Rockefeller – U.S. Senator from West Virginia^[24]
• Franklin Delano Roosevelt – 32nd President of the United States^[5]
• James Roosevelt – U.S. Congressman (CA), 1955–1965^[25]
• Theodore Roosevelt – 26th President of the United States^[1][5]
• William Weld – 68th Governor of Massachusetts^[26]

• Phillips Brooks – clergyman, author, lyricist^[5]
• Edward Everett Hale – author, historian, Unitarian minister, Chaplain to the U.S. Senate^[1]
• William Appleton Lawrence – clergyman, 3rd bishop of the Episcopal Diocese of Western Massachusetts^[27]

• Francis Cabot – gardener, horticulturist, chairman of the New York Botanical Garden, and founder of the Garden Conservancy^[28]
• Michael Clark Rockefeller – amateur anthropologist, disappeared in 1961 during an expedition in Netherlands New Guinea.

• Charles Francis Adams III – skipper of America's Cup defender Resolute, 1920; inductee, America's Cup Hall of Fame; Secretary of the Navy, 1929–1932^[1]
• Charles Dudley Daly – college football player and coach who was inducted into the College Football Hall of Fame
• Dwight F. Davis – Olympic tennis player; three-time U.S. Open doubles champion; founder of the Davis Cup; International Tennis Hall of Fame inductee^[1][5][8]
• W. Palmer Dixon – two-time winner of national squash championship (1925, 1926)^[29]
• Matt Freese – professional soccer player with New York City FC
• Henry Thrun – professional ice hockey player for the San Jose Sharks, winner of a gold medal at 2021 World Junior Championship.^[30]