In enzymology, a leucine N-acetyltransferase (EC 2.3.1.66) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + L-leucine ${\displaystyle \rightleftharpoons }$ CoA + N-acetyl-L-leucine

Thus, the two substrates of this enzyme are acetyl-CoA and L-leucine, whereas its two products are CoA and N-acetyl-L-leucine.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:L-leucine N-acetyltransferase. This enzyme is also called leucine acetyltransferase.

• Suzukake S, Hayashi H, Hori M, Umezawa H (1982). "Biosnthesis of leupeptin III. Isolation and properties of an enzyme synthesizing acetyl-L-leucine". J. Antibiot. 33 (8): 857–862. doi:10.7164/antibiotics.33.857. PMID 7429989.