In enzymology, a glutathione—CoA-glutathione transhydrogenase (EC 1.8.4.3) is an enzyme that catalyzes the chemical reaction

CoA + glutathione disulfide ${\displaystyle \rightleftharpoons }$ CoA-glutathione + glutathione

Thus, the two substrates of this enzyme are CoA and glutathione disulfide, whereas its two products are CoA-glutathione and glutathione.

This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with a disulfide as acceptor. The systematic name of this enzyme class is CoA:glutathione-disulfide oxidoreductase. Other names in common use include glutathione-coenzyme A glutathione disulfide transhydrogenase, glutathione-coenzyme A glutathione disulfide transhydrogenase, glutathione coenzyme A-glutathione transhydrogenase, glutathione:coenzyme A-glutathione transhydrogenase, coenzyme A:oxidized-glutathione oxidoreductase, and coenzyme A:glutathione-disulfide oxidoreductase. This enzyme participates in cysteine metabolism and glutathione metabolism.

• Chang SH, Wilken DR (1966). "Participation of the unsymmetrical disulfide of coenzyme A and glutathione in an enzymatic sulfhydryl-disulfide interchange. I Partial purification and properties of the bovine kidney enzyme". J. Biol. Chem. 241 (18): 4251–60. PMID 5924646.