Gellan tetrasaccharide unsaturated glucuronyl hydrolase

Gellan tetrasaccharide unsaturated glucuronyl hydrolase
Identifiers
EC no.3.2.1.179
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins

Gellan tetrasaccharide unsaturated glucuronyl hydrolase (EC 3.2.1.179, UGL, unsaturated glucuronyl hydrolase) is an enzyme with systematic name beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp beta-D-4-deoxy-Delta4-GlcAp hydrolase.[1][2][3] This enzyme catalyses the following chemical reaction

beta-D-4-deoxy-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp + H2O 5-dehydro-4-deoxy-D-glucuronate + beta-D-Glcp-(1->4)-alpha-L-Rhap-(1->3)-beta-D-Glcp

The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases.

References

  1. ^ Itoh T, Akao S, Hashimoto W, Mikami B, Murata K (July 2004). "Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A resolution". The Journal of Biological Chemistry. 279 (30): 31804–12. doi:10.1074/jbc.m403288200. PMID 15148314.
  2. ^ Hashimoto W, Kobayashi E, Nankai H, Sato N, Miya T, Kawai S, Murata K (August 1999). "Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases". Archives of Biochemistry and Biophysics. 368 (2): 367–74. doi:10.1006/abbi.1999.1305. PMID 10441389.
  3. ^ Itoh T, Hashimoto W, Mikami B, Murata K (May 2006). "Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1". Biochemical and Biophysical Research Communications. 344 (1): 253–62. doi:10.1016/j.bbrc.2006.03.141. PMID 16630576.