In enzymology, a dicarboxylate—CoA ligase (EC 6.2.1.23) is an enzyme that catalyzes the chemical reaction

ATP + an alphaomega-dicarboxylic acid + CoA ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + an omega-carboxyacyl-CoA

The 3 substrates of this enzyme are ATP, alphaomega-dicarboxylic acid, and CoA, whereas its 3 products are AMP, diphosphate, and omega-carboxyacyl-CoA.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is omega-dicarboxylate:CoA ligase (AMP-forming). Other names in common use include carboxylyl-CoA synthetase, and dicarboxylyl-CoA synthetase.

• Vamecq J, de Hoffmann E, Van Hoof F (1985). "The microsomal dicarboxylyl-CoA synthetase". Biochem. J. 230 (3): 683–93. PMC 1152672. PMID 4062873.